Restriction map for
pF1KB7220
(Linear) (Six-base) MAPSORT of: KIBB7220.seq Check: 1210 from: 1 to: 957
KIBB7220.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 957
Size: 574 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 339 957
Size: 339 618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 957
Size: 416 32 509
Fragments arranged by size:
509 416 32
ApoI r'AATT_y
Cuts at: 0 518 957
Size: 518 439
BclI T'GATC_A
Cuts at: 0 272 957
Size: 272 685
BlpI GC'TnA_GC
Cuts at: 0 947 957
Size: 947 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 907 957
Size: 907 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 957
Size: 493 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 957
Size: 469 488
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 67 657 898 901 957
Size: 28 39 590 241 3 56
Fragments arranged by size:
590 241 56 39 28 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 489 957
Size: 373 116 468
Fragments arranged by size:
468 373 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 929 957
Size: 929 28
BspHI T'CATG_A
Cuts at: 0 404 957
Size: 404 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 957
Size: 134 823
BsrDI GCAATG_nn'
Cuts at: 0 311 941 957
Size: 311 630 16
Fragments arranged by size:
630 311 16
BsrGI T'GTAC_A
Cuts at: 0 827 957
Size: 827 130
BstEII G'GTnAC_C
Cuts at: 0 108 837 957
Size: 108 729 120
Fragments arranged by size:
729 120 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 615 957
Size: 105 510 342
Fragments arranged by size:
510 342 105
Bsu36I CC'TnA_GG
Cuts at: 0 871 913 957
Size: 871 42 44
Fragments arranged by size:
871 44 42
BtgI C'CryG_G
Cuts at: 0 81 833 957
Size: 81 752 124
Fragments arranged by size:
752 124 81
EaeI y'GGCC_r
Cuts at: 0 371 957
Size: 371 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 957
Size: 574 383
EcoO109I rG'GnC_Cy
Cuts at: 0 440 957
Size: 440 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 957
Size: 896 32 29
Fragments arranged by size:
896 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 215 957
Size: 6 209 742
Fragments arranged by size:
742 209 6
MscI TGG'CCA
Cuts at: 0 373 957
Size: 373 584
MslI CAynn'nnrTG
Cuts at: 0 172 321 511 592 844 957
Size: 172 149 190 81 252 113
Fragments arranged by size:
252 190 172 149 113 81
NcoI C'CATG_G
Cuts at: 0 81 833 957
Size: 81 752 124
Fragments arranged by size:
752 124 81
NspI r_CATG'y
Cuts at: 0 69 957
Size: 69 888
PpuMI rG'GwC_Cy
Cuts at: 0 440 957
Size: 440 517
PshAI GACnn'nnGTC
Cuts at: 0 610 957
Size: 610 347
PstI C_TGCA'G
Cuts at: 0 786 957
Size: 786 171
SexAI A'CCwGG_T
Cuts at: 0 104 957
Size: 104 853
SfcI C'TryA_G
Cuts at: 0 782 864 957
Size: 782 82 93
Fragments arranged by size:
782 93 82
SmlI C'TyrA_G
Cuts at: 0 472 957
Size: 472 485
StyI C'CwwG_G
Cuts at: 0 81 762 833 957
Size: 81 681 71 124
Fragments arranged by size:
681 124 81 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 957
Size: 607 350
TatI w'GTAC_w
Cuts at: 0 176 827 957
Size: 176 651 130
Fragments arranged by size:
651 176 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 22 957
Size: 22 935
XmnI GAAnn'nnTTC
Cuts at: 0 856 957
Size: 856 101
Enzymes that do cut:
AcuI AlfI AloI ApoI BclI BlpI BmrI BpuEI
BsaI BseRI BsgI BsmBI BspHI BspMI BsrDI BsrGI
BstEII BstXI Bsu36I BtgI EaeI Eco57MI EcoO109I Hin4I
MmeI MscI MslI NcoI NspI PpuMI PshAI PstI
SexAI SfcI SmlI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI