Restriction map for
pF1KB7219
(Linear) (Six-base) MAPSORT of: KIBB7219.seq Check: 2774 from: 1 to: 960
KIBB7219.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 727 960
Size: 170 557 233
Fragments arranged by size:
557 233 170
AccI GT'mk_AC
Cuts at: 0 25 960
Size: 25 935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 960
Size: 713 247
ApoI r'AATT_y
Cuts at: 0 31 539 960
Size: 31 508 421
Fragments arranged by size:
508 421 31
BanI G'GyrC_C
Cuts at: 0 415 960
Size: 415 545
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 960
Size: 824 136
BglI GCCn_nnn'nGGC
Cuts at: 0 322 424 960
Size: 322 102 536
Fragments arranged by size:
536 322 102
BlpI GC'TnA_GC
Cuts at: 0 723 960
Size: 723 237
BmgBI CAC'GTC
Cuts at: 0 23 960
Size: 23 937
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 960
Size: 461 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 960
Size: 52 908
BseYI C'CCAG_C
Cuts at: 0 436 960
Size: 436 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 493 960
Size: 148 345 467
Fragments arranged by size:
467 345 148
BspHI T'CATG_A
Cuts at: 0 404 960
Size: 404 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 727 960
Size: 170 557 233
Fragments arranged by size:
557 233 170
BsrDI GCAATG_nn'
Cuts at: 0 494 960
Size: 494 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 960
Size: 255 705
BtgI C'CryG_G
Cuts at: 0 317 326 428 960
Size: 317 9 102 532
Fragments arranged by size:
532 317 102 9
DraIII CAC_nnn'GTG
Cuts at: 0 332 960
Size: 332 628
EaeI y'GGCC_r
Cuts at: 0 371 425 960
Size: 371 54 535
Fragments arranged by size:
535 371 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 713 960
Size: 52 661 247
Fragments arranged by size:
661 247 52
EcoO109I rG'GnC_Cy
Cuts at: 0 440 831 960
Size: 440 391 129
Fragments arranged by size:
440 391 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 960
Size: 294 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 119 151 383 415 960
Size: 7 32 80 32 232 32 545
Fragments arranged by size:
545 232 80 32 32 32 7
HincII GTy'rAC
Cuts at: 0 26 960
Size: 26 934
MscI TGG'CCA
Cuts at: 0 373 427 960
Size: 373 54 533
Fragments arranged by size:
533 373 54
MslI CAynn'nnrTG
Cuts at: 0 172 960
Size: 172 788
MspA1I CmG'CkG
Cuts at: 0 436 960
Size: 436 524
NcoI C'CATG_G
Cuts at: 0 317 428 960
Size: 317 111 532
Fragments arranged by size:
532 317 111
PpuMI rG'GwC_Cy
Cuts at: 0 440 960
Size: 440 520
PvuII CAG'CTG
Cuts at: 0 436 960
Size: 436 524
SalI G'TCGA_C
Cuts at: 0 24 960
Size: 24 936
SfcI C'TryA_G
Cuts at: 0 885 960
Size: 885 75
StuI AGG'CCT
Cuts at: 0 709 960
Size: 709 251
StyI C'CwwG_G
Cuts at: 0 317 428 960
Size: 317 111 532
Fragments arranged by size:
532 317 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 74 275 960
Size: 74 201 685
Fragments arranged by size:
685 201 74
Tth111I GACn'n_nGTC
Cuts at: 0 600 960
Size: 600 360
Enzymes that do cut:
AarI AccI AcuI ApoI BanI BbsI BglI BlpI
BmgBI BmrI BpmI BseYI BsgI BspHI BspMI BsrDI
BstXI BtgI DraIII EaeI Eco57MI EcoO109I FalI Hin4I
HincII MscI MslI MspA1I NcoI PpuMI PvuII SalI
SfcI StuI StyI TaqII Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglII Bme1580I BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XcmI XhoI XmaI XmnI ZraI