Restriction map for
pF1KB7218
(Linear) (Six-base) MAPSORT of: KIBB7218.seq Check: 7127 from: 1 to: 750
KIBB7218.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 698 750
Size: 698 52
AleI CACnn'nnGTG
Cuts at: 0 486 750
Size: 486 264
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 750
Size: 244 32 474
Fragments arranged by size:
474 244 32
ApaI G_GGCC'C
Cuts at: 0 619 750
Size: 619 131
BamHI G'GATC_C
Cuts at: 0 728 750
Size: 728 22
BanI G'GyrC_C
Cuts at: 0 686 750
Size: 686 64
BanII G_rGCy'C
Cuts at: 0 619 671 750
Size: 619 52 79
Fragments arranged by size:
619 79 52
BbeI G_GCGC'C
Cuts at: 0 690 750
Size: 690 60
BbvCI CC'TCA_GC
Cuts at: 0 307 394 750
Size: 307 87 356
Fragments arranged by size:
356 307 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 578 612 750
Size: 578 34 138
Fragments arranged by size:
578 138 34
BglI GCCn_nnn'nGGC
Cuts at: 0 73 750
Size: 73 677
BlpI GC'TnA_GC
Cuts at: 0 665 750
Size: 665 85
Bme1580I G_kGCm'C
Cuts at: 0 619 750
Size: 619 131
BmgBI CAC'GTC
Cuts at: 0 364 750
Size: 364 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 537 750
Size: 537 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 750
Size: 690 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 660 750
Size: 529 131 90
Fragments arranged by size:
529 131 90
Bpu10I CC'TnA_GC
Cuts at: 0 307 394 750
Size: 307 87 356
Fragments arranged by size:
356 307 87
BsaHI Gr'CG_yC
Cuts at: 0 687 750
Size: 687 63
BsaWI w'CCGG_w
Cuts at: 0 249 750
Size: 249 501
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 750
Size: 244 30 476
Fragments arranged by size:
476 244 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 750
Size: 4 746
BseYI C'CCAG_C
Cuts at: 0 6 50 98 440 750
Size: 6 44 48 342 310
Fragments arranged by size:
342 310 48 44 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 750
Size: 400 350
BsiHKAI G_wGCw'C
Cuts at: 0 24 227 750
Size: 24 203 523
Fragments arranged by size:
523 203 24
Bsp1286I G_dGCh'C
Cuts at: 0 24 227 619 671 750
Size: 24 203 392 52 79
Fragments arranged by size:
392 203 79 52 24
BspEI T'CCGG_A
Cuts at: 0 249 750
Size: 249 501
BsrDI GCAATG_nn'
Cuts at: 0 636 750
Size: 636 114
BsrFI r'CCGG_y
Cuts at: 0 112 750
Size: 112 638
BstYI r'GATC_y
Cuts at: 0 728 750
Size: 728 22
BtsI GCAGTG_nn'
Cuts at: 0 186 430 481 493 750
Size: 186 244 51 12 257
Fragments arranged by size:
257 244 186 51 12
EarI CTCTTCn'nnn_
Cuts at: 0 124 142 750
Size: 124 18 608
Fragments arranged by size:
608 124 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 334 750
Size: 202 132 416
Fragments arranged by size:
416 202 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 660 750
Size: 529 131 90
Fragments arranged by size:
529 131 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 750
Size: 577 173
EcoO109I rG'GnC_Cy
Cuts at: 0 303 615 616 750
Size: 303 312 1 134
Fragments arranged by size:
312 303 134 1
FseI GG_CCGG'CC
Cuts at: 0 116 750
Size: 116 634
HaeII r_GCGC'y
Cuts at: 0 314 469 690 750
Size: 314 155 221 60
Fragments arranged by size:
314 221 155 60
KasI G'GCGC_C
Cuts at: 0 686 750
Size: 686 64
MslI CAynn'nnrTG
Cuts at: 0 326 486 750
Size: 326 160 264
Fragments arranged by size:
326 264 160
MspA1I CmG'CkG
Cuts at: 0 167 181 258 750
Size: 167 14 77 492
Fragments arranged by size:
492 167 77 14
NaeI GCC'GGC
Cuts at: 0 114 750
Size: 114 636
NarI GG'CG_CC
Cuts at: 0 687 750
Size: 687 63
NgoMIV G'CCGG_C
Cuts at: 0 112 750
Size: 112 638
NspI r_CATG'y
Cuts at: 0 423 561 750
Size: 423 138 189
Fragments arranged by size:
423 189 138
PfoI T'CCnGG_A
Cuts at: 0 506 750
Size: 506 244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 750
Size: 244 32 474
Fragments arranged by size:
474 244 32
PpuMI rG'GwC_Cy
Cuts at: 0 303 750
Size: 303 447
PshAI GACnn'nnGTC
Cuts at: 0 406 750
Size: 406 344
PspOMI G'GGCC_C
Cuts at: 0 615 750
Size: 615 135
PstI C_TGCA'G
Cuts at: 0 488 750
Size: 488 262
PvuII CAG'CTG
Cuts at: 0 181 258 750
Size: 181 77 492
Fragments arranged by size:
492 181 77
SapI GCTCTTCn'nnn_
Cuts at: 0 142 750
Size: 142 608
SfcI C'TryA_G
Cuts at: 0 484 750
Size: 484 266
SfoI GGC'GCC
Cuts at: 0 688 750
Size: 688 62
SphI G_CATG'C
Cuts at: 0 423 561 750
Size: 423 138 189
Fragments arranged by size:
423 189 138
StyI C'CwwG_G
Cuts at: 0 103 216 461 638 750
Size: 103 113 245 177 112
Fragments arranged by size:
245 177 113 112 103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 607 750
Size: 607 143
Enzymes that do cut:
AccI AleI AloI ApaI BamHI BanI BanII BbeI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BsrDI BsrFI BstYI BtsI EarI
EciI Eco57MI EcoNI EcoO109I FseI HaeII KasI MslI
MspA1I NaeI NarI NgoMIV NspI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SapI SfcI SfoI SphI
StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbsI BciVI BclI BfrBI
BglII BmtI BpuEI BsaI BsaAI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI