(Linear) (Six-base) MAPSORT of: KIBB7216.seq  Check: 5690  from: 1  to: 447



KIBB7216.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AfeI AGC'GCT

Cuts at:      0      11     447 
   Size:         11     436
 
AhdI GACnn_n'nnGTC

Cuts at:      0      73     447 
   Size:         73     374
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76     447 
   Size:         76     371
 
ApaI G_GGCC'C

Cuts at:      0     206     447 
   Size:        206     241
 
AvaI C'yCGr_G

Cuts at:      0     178     447 
   Size:        178     269
 
AvrII C'CTAG_G

Cuts at:      0     111     447 
   Size:        111     336
 
BanI G'GyrC_C

Cuts at:      0     115     165     447 
   Size:        115      50     282

  Fragments arranged by size: 

                 282     115      50
 
BanII G_rGCy'C

Cuts at:      0     206     263     394     447 
   Size:        206      57     131      53

  Fragments arranged by size: 

                 206     131      57      53
 
Bme1580I G_kGCm'C

Cuts at:      0     206     289     447 
   Size:        206      83     158

  Fragments arranged by size: 

                 206     158      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     265     447 
   Size:         70     195     182

  Fragments arranged by size: 

                 195     182      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     262     447 
   Size:        262     185
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     164     447 
   Size:        164     283
 
BsaWI w'CCGG_w

Cuts at:      0     358     447 
   Size:        358      89
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     447 
   Size:        263     184
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      81     447 
   Size:         81     366
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     263     289     394     447 
   Size:        206      57      26     105      53

  Fragments arranged by size: 

                 206     105      57      53      26
 
BssSI C'ACGA_G

Cuts at:      0      54     447 
   Size:         54     393
 
BtsI GCAGTG_nn'

Cuts at:      0      42     447 
   Size:         42     405
 
DraIII CAC_nnn'GTG

Cuts at:      0     295     447 
   Size:        295     152
 
EcoICRI GAG'CTC

Cuts at:      0     261     447 
   Size:        261     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     265     447 
   Size:         70     195     182

  Fragments arranged by size: 

                 195     182      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     124     447 
   Size:        124     323
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     203     447 
   Size:         91     112     244

  Fragments arranged by size: 

                 244     112      91
 
HaeII r_GCGC'y

Cuts at:      0      13     447 
   Size:         13     434
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      59      91     263     295     447 
   Size:         59      32     172      32     152

  Fragments arranged by size: 

                 172     152      59      32      32
 
HindIII A'AGCT_T

Cuts at:      0     139     447 
   Size:        139     308
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     447 
   Size:        115     332
 
MspA1I CmG'CkG

Cuts at:      0      32     369     447 
   Size:         32     337      78

  Fragments arranged by size: 

                 337      78      32
 
PasI CC'CwG_GG

Cuts at:      0     120     447 
   Size:        120     327
 
PpuMI rG'GwC_Cy

Cuts at:      0      91     447 
   Size:         91     356
 
PspOMI G'GGCC_C

Cuts at:      0     202     447 
   Size:        202     245
 
PstI C_TGCA'G

Cuts at:      0      37     447 
   Size:         37     410
 
PvuII CAG'CTG

Cuts at:      0     369     447 
   Size:        369      78
 
SacI G_AGCT'C

Cuts at:      0     263     447 
   Size:        263     184
 
SanDI GG'GwC_CC

Cuts at:      0      91     447 
   Size:         91     356
 
SfcI C'TryA_G

Cuts at:      0      33     447 
   Size:         33     414
 
StyI C'CwwG_G

Cuts at:      0     111     447 
   Size:        111     336
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     230     447 
   Size:        208      22     217

  Fragments arranged by size: 

                 217     208      22
 

 Enzymes that do cut:

     AfeI     AhdI     AlfI     ApaI     AvaI    AvrII     BanI    BanII
 Bme1580I     BpmI   Bpu10I     BsaI    BsaWI  BsiHKAI    BsmBI Bsp1286I
    BssSI     BtsI   DraIII  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I  HindIII     MmeI   MspA1I     PasI    PpuMI   PspOMI     PstI
    PvuII     SacI    SanDI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI