(Linear) (Six-base) MAPSORT of: KIBB7215.seq  Check: 6192  from: 1  to: 333



KIBB7215.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     114     333 
   Size:        114     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     135     333 
   Size:        135     198
 
Bpu10I CC'TnA_GC

Cuts at:      0     275     333 
   Size:        275      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     280     310     333 
   Size:        280      30      23

  Fragments arranged by size: 

                 280      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     297     300     333 
   Size:        297       3      33

  Fragments arranged by size: 

                 297      33       3
 
BseYI C'CCAG_C

Cuts at:      0     102     333 
   Size:        102     231
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     333 
   Size:        175     158
 
BsiWI C'GTAC_G

Cuts at:      0     114     333 
   Size:        114     219
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     333 
   Size:        197     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     278     315     333 
   Size:        278      37      18

  Fragments arranged by size: 

                 278      37      18
 
MspA1I CmG'CkG

Cuts at:      0     106     333 
   Size:        106     227
 
PvuII CAG'CTG

Cuts at:      0     106     333 
   Size:        106     227
 
SexAI A'CCwGG_T

Cuts at:      0     316     333 
   Size:        316      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195     280     312     333 
   Size:        163      32      85      32      21

  Fragments arranged by size: 

                 163      85      32      32      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      15     333 
   Size:         15     318
 

 Enzymes that do cut:

     AlfI     BmrI   Bpu10I    BsaXI    BseRI    BseYI     BsgI    BsiWI
    BspMI    EcoNI   MspA1I    PvuII    SexAI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI