(Linear) (Six-base) MAPSORT of: KIBB7213.seq  Check: 2089  from: 1  to: 426



KIBB7213.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     307     426 
   Size:        307     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0     309     426 
   Size:        309     117
 
ApaI G_GGCC'C

Cuts at:      0     413     426 
   Size:        413      13
 
ApaLI G'TGCA_C

Cuts at:      0     402     426 
   Size:        402      24
 
BanI G'GyrC_C

Cuts at:      0     361     426 
   Size:        361      65
 
BanII G_rGCy'C

Cuts at:      0     236     413     426 
   Size:        236     177      13

  Fragments arranged by size: 

                 236     177      13
 
Bme1580I G_kGCm'C

Cuts at:      0     364     406     413     426 
   Size:        364      42       7      13

  Fragments arranged by size: 

                 364      42      13       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     426 
   Size:        235     191
 
BsaAI yAC'GTr

Cuts at:      0      15     426 
   Size:         15     411
 
BsaHI Gr'CG_yC

Cuts at:      0     210     426 
   Size:        210     216
 
BseYI C'CCAG_C

Cuts at:      0     222     426 
   Size:        222     204
 
BsiHKAI G_wGCw'C

Cuts at:      0     406     426 
   Size:        406      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     364     406     413     426 
   Size:        236     128      42       7      13

  Fragments arranged by size: 

                 236     128      42      13       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     307     322     426 
   Size:        307      15     104

  Fragments arranged by size: 

                 307     104      15
 
BsrFI r'CCGG_y

Cuts at:      0     159     286     426 
   Size:        159     127     140

  Fragments arranged by size: 

                 159     140     127
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     281     426 
   Size:        281     145
 
BstEII G'GTnAC_C

Cuts at:      0      48     426 
   Size:         48     378
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      99     426 
   Size:         99     327
 
EaeI y'GGCC_r

Cuts at:      0     157     426 
   Size:        157     269
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      93     426 
   Size:         93     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     426 
   Size:        235     191
 
MspA1I CmG'CkG

Cuts at:      0     306     426 
   Size:        306     120
 
NaeI GCC'GGC

Cuts at:      0     288     426 
   Size:        288     138
 
NgoMIV G'CCGG_C

Cuts at:      0     286     426 
   Size:        286     140
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      18     426 
   Size:         18     408
 
PmlI CAC'GTG

Cuts at:      0      15     426 
   Size:         15     411
 
PspOMI G'GGCC_C

Cuts at:      0     409     426 
   Size:        409      17
 
PstI C_TGCA'G

Cuts at:      0     113     426 
   Size:        113     313
 
SexAI A'CCwGG_T

Cuts at:      0      59     426 
   Size:         59     367
 
SfcI C'TryA_G

Cuts at:      0     109     426 
   Size:        109     317
 
TatI w'GTAC_w

Cuts at:      0     113     426 
   Size:        113     313
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI    ApaLI     BanI    BanII Bme1580I     BpmI
    BsaAI    BsaHI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI
   BstEII     DrdI     EaeI     EciI  Eco57MI   MspA1I     NaeI   NgoMIV
    PflMI     PmlI   PspOMI     PstI    SexAI     SfcI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI