Restriction map for
pF1KB7206
(Linear) (Six-base) MAPSORT of: KIBB7206.seq Check: 1571 from: 1 to: 1725
KIBB7206.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AatII G_ACGT'C
Cuts at: 0 860 1725
Size: 860 865
AccI GT'mk_AC
Cuts at: 0 623 1725
Size: 623 1102
AfeI AGC'GCT
Cuts at: 0 1370 1725
Size: 1370 355
AflIII A'CryG_T
Cuts at: 0 1694 1725
Size: 1694 31
AleI CACnn'nnGTG
Cuts at: 0 643 1725
Size: 643 1082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 1725
Size: 32 1693
AlwNI CAG_nnn'CTG
Cuts at: 0 310 1332 1725
Size: 310 1022 393
Fragments arranged by size:
1022 393 310
ApaI G_GGCC'C
Cuts at: 0 385 1725
Size: 385 1340
ApoI r'AATT_y
Cuts at: 0 1003 1459 1725
Size: 1003 456 266
Fragments arranged by size:
1003 456 266
AvaI C'yCGr_G
Cuts at: 0 289 587 1297 1725
Size: 289 298 710 428
Fragments arranged by size:
710 428 298 289
AvrII C'CTAG_G
Cuts at: 0 777 1725
Size: 777 948
BamHI G'GATC_C
Cuts at: 0 1322 1725
Size: 1322 403
BanI G'GyrC_C
Cuts at: 0 42 1725
Size: 42 1683
BanII G_rGCy'C
Cuts at: 0 385 1168 1269 1725
Size: 385 783 101 456
Fragments arranged by size:
783 456 385 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 1525 1725
Size: 1525 200
BbvCI CC'TCA_GC
Cuts at: 0 535 1725
Size: 535 1190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 151 185 1725
Size: 151 34 1540
Fragments arranged by size:
1540 151 34
BfrBI ATG'CAT
Cuts at: 0 268 1725
Size: 268 1457
BglI GCCn_nnn'nGGC
Cuts at: 0 284 373 446 455 1725
Size: 284 89 73 9 1270
Fragments arranged by size:
1270 284 89 73 9
BlpI GC'TnA_GC
Cuts at: 0 173 1717 1725
Size: 173 1544 8
Fragments arranged by size:
1544 173 8
Bme1580I G_kGCm'C
Cuts at: 0 47 385 403 1725
Size: 47 338 18 1322
Fragments arranged by size:
1322 338 47 18
BmgBI CAC'GTC
Cuts at: 0 1449 1695 1725
Size: 1449 246 30
Fragments arranged by size:
1449 246 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 380 1270 1725
Size: 94 286 890 455
Fragments arranged by size:
890 455 286 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 484 829 1069 1510 1725
Size: 484 345 240 441 215
Fragments arranged by size:
484 441 345 240 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 380 425 963 1030 1064 1444 1534
Size: 365 15 45 538 67 34 380 90
Cuts at: 1534 1725
Size: 191
Fragments arranged by size:
538 380 365 191 90 67 45 34
15
Bpu10I CC'TnA_GC
Cuts at: 0 535 628 1725
Size: 535 93 1097
Fragments arranged by size:
1097 535 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1725
Size: 229 1496
BsaI GGTCTCn'nnnn_
Cuts at: 0 148 472 1410 1725
Size: 148 324 938 315
Fragments arranged by size:
938 324 315 148
BsaHI Gr'CG_yC
Cuts at: 0 857 1643 1725
Size: 857 786 82
Fragments arranged by size:
857 786 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 488 518 961 991 1725
Size: 488 30 443 30 734
Fragments arranged by size:
734 488 443 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 509 1061 1725
Size: 509 552 664
Fragments arranged by size:
664 552 509
BseYI C'CCAG_C
Cuts at: 0 177 396 897 918 1136 1409 1632 1725
Size: 177 219 501 21 218 273 223 93
Fragments arranged by size:
501 273 223 219 218 177 93 21
BsiHKAI G_wGCw'C
Cuts at: 0 518 1725
Size: 518 1207
BsmI GAATG_Cn'
Cuts at: 0 270 1606 1725
Size: 270 1336 119
Fragments arranged by size:
1336 270 119
Bsp1286I G_dGCh'C
Cuts at: 0 47 385 403 518 1168 1269 1725
Size: 47 338 18 115 650 101 456
Fragments arranged by size:
650 456 338 115 101 47 18
BspHI T'CATG_A
Cuts at: 0 1496 1725
Size: 1496 229
BsrDI GCAATG_nn'
Cuts at: 0 1625 1686 1725
Size: 1625 61 39
Fragments arranged by size:
1625 61 39
BsrFI r'CCGG_y
Cuts at: 0 326 1725
Size: 326 1399
BsrGI T'GTAC_A
Cuts at: 0 635 1725
Size: 635 1090
BssSI C'ACGA_G
Cuts at: 0 823 925 1725
Size: 823 102 800
Fragments arranged by size:
823 800 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1073 1161 1422 1725
Size: 825 248 88 261 303
Fragments arranged by size:
825 303 261 248 88
BstYI r'GATC_y
Cuts at: 0 203 1322 1725
Size: 203 1119 403
Fragments arranged by size:
1119 403 203
BtgI C'CryG_G
Cuts at: 0 725 1115 1725
Size: 725 390 610
Fragments arranged by size:
725 610 390
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 669 777 1725
Size: 669 108 948
Fragments arranged by size:
948 669 108
BtsI GCAGTG_nn'
Cuts at: 0 167 261 1388 1725
Size: 167 94 1127 337
Fragments arranged by size:
1127 337 167 94
DrdI GACnn_nn'nnGTC
Cuts at: 0 82 1725
Size: 82 1643
EaeI y'GGCC_r
Cuts at: 0 647 851 1075 1142 1385 1725
Size: 647 204 224 67 243 340
Fragments arranged by size:
647 340 243 224 204 67
EarI CTCTTCn'nnn_
Cuts at: 0 655 1226 1725
Size: 655 571 499
Fragments arranged by size:
655 571 499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 473 1725
Size: 473 1252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 380 425 963 1030 1064 1444 1534
Size: 365 15 45 538 67 34 380 90
Cuts at: 1534 1725
Size: 191
Fragments arranged by size:
538 380 365 191 90 67 45 34
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 1573 1725
Size: 37 1536 152
Fragments arranged by size:
1536 152 37
EcoO109I rG'GnC_Cy
Cuts at: 0 91 231 285 1704 1725
Size: 91 140 54 1419 21
Fragments arranged by size:
1419 140 91 54 21
EcoRI G'AATT_C
Cuts at: 0 1003 1459 1725
Size: 1003 456 266
Fragments arranged by size:
1003 456 266
EcoRV GAT'ATC
Cuts at: 0 1212 1725
Size: 1212 513
HaeII r_GCGC'y
Cuts at: 0 1372 1725
Size: 1372 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 488 520 570 602 1040 1072 1256 1288
Size: 488 32 50 32 438 32 184 32
Cuts at: 1288 1725
Size: 437
Fragments arranged by size:
488 438 437 184 50 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1679 1725
Size: 1679 46
MscI TGG'CCA
Cuts at: 0 649 1077 1144 1387 1725
Size: 649 428 67 243 338
Fragments arranged by size:
649 428 338 243 67
MslI CAynn'nnrTG
Cuts at: 0 643 1071 1447 1576 1693 1725
Size: 643 428 376 129 117 32
Fragments arranged by size:
643 428 376 129 117 32
MspA1I CmG'CkG
Cuts at: 0 177 421 918 1081 1140 1725
Size: 177 244 497 163 59 585
Fragments arranged by size:
585 497 244 177 163 59
NcoI C'CATG_G
Cuts at: 0 725 1115 1725
Size: 725 390 610
Fragments arranged by size:
725 610 390
NdeI CA'TA_TG
Cuts at: 0 270 1725
Size: 270 1455
NsiI A_TGCA'T
Cuts at: 0 270 1725
Size: 270 1455
NspI r_CATG'y
Cuts at: 0 414 1198 1227 1725
Size: 414 784 29 498
Fragments arranged by size:
784 498 414 29
PfoI T'CCnGG_A
Cuts at: 0 677 1007 1725
Size: 677 330 718
Fragments arranged by size:
718 677 330
PpuMI rG'GwC_Cy
Cuts at: 0 91 231 1704 1725
Size: 91 140 1473 21
Fragments arranged by size:
1473 140 91 21
PspOMI G'GGCC_C
Cuts at: 0 381 1725
Size: 381 1344
PstI C_TGCA'G
Cuts at: 0 8 341 1187 1358 1725
Size: 8 333 846 171 367
Fragments arranged by size:
846 367 333 171 8
PvuII CAG'CTG
Cuts at: 0 177 421 918 1081 1140 1725
Size: 177 244 497 163 59 585
Fragments arranged by size:
585 497 244 177 163 59
SapI GCTCTTCn'nnn_
Cuts at: 0 1226 1725
Size: 1226 499
SfcI C'TryA_G
Cuts at: 0 4 337 1183 1354 1725
Size: 4 333 846 171 371
Fragments arranged by size:
846 371 333 171 4
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 284 446 1725
Size: 284 162 1279
Fragments arranged by size:
1279 284 162
SgrAI Cr'CCGG_yG
Cuts at: 0 326 1725
Size: 326 1399
SmlI C'TyrA_G
Cuts at: 0 208 1725
Size: 208 1517
SphI G_CATG'C
Cuts at: 0 414 1198 1725
Size: 414 784 527
Fragments arranged by size:
784 527 414
StyI C'CwwG_G
Cuts at: 0 47 441 471 710 725 777 1115 1277
Size: 47 394 30 239 15 52 338 162
Cuts at: 1277 1725
Size: 448
Fragments arranged by size:
448 394 338 239 162 52 47 30
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 607 1108 1725
Size: 40 567 501 617
Fragments arranged by size:
617 567 501 40
TatI w'GTAC_w
Cuts at: 0 635 968 1725
Size: 635 333 757
Fragments arranged by size:
757 635 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 1725
Size: 726 32 967
Fragments arranged by size:
967 726 32
XbaI T'CTAG_A
Cuts at: 0 1200 1725
Size: 1200 525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 888 1137 1257 1725
Size: 375 513 249 120 468
Fragments arranged by size:
513 468 375 249 120
XmnI GAAnn'nnTTC
Cuts at: 0 1235 1725
Size: 1235 490
ZraI GAC'GTC
Cuts at: 0 858 1725
Size: 858 867
Enzymes that do cut:
AatII AccI AfeI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PfoI PpuMI PspOMI PstI PvuII SapI SfcI SfiI
SgrAI SmlI SphI StyI TaqII TatI TstI XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI BaeI BbeI BciVI BclI
BglII BmtI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoICRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfoI SgfI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XhoI
XmaI