Restriction map for
pF1KB7203
(Linear) (Six-base) MAPSORT of: KIBB7203.seq Check: 3382 from: 1 to: 1869
KIBB7203.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 711 1359 1639 1869
Size: 219 492 648 280 230
Fragments arranged by size:
648 492 280 230 219
AfeI AGC'GCT
Cuts at: 0 1663 1869
Size: 1663 206
AgeI A'CCGG_T
Cuts at: 0 547 1869
Size: 547 1322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 633 829 1869
Size: 633 196 1040
Fragments arranged by size:
1040 633 196
AlwNI CAG_nnn'CTG
Cuts at: 0 1506 1869
Size: 1506 363
ApaI G_GGCC'C
Cuts at: 0 660 760 848 1869
Size: 660 100 88 1021
Fragments arranged by size:
1021 660 100 88
ApaLI G'TGCA_C
Cuts at: 0 895 1869
Size: 895 974
AvaI C'yCGr_G
Cuts at: 0 56 343 847 1869
Size: 56 287 504 1022
Fragments arranged by size:
1022 504 287 56
AvrII C'CTAG_G
Cuts at: 0 585 1869
Size: 585 1284
BanI G'GyrC_C
Cuts at: 0 1347 1509 1869
Size: 1347 162 360
Fragments arranged by size:
1347 360 162
BanII G_rGCy'C
Cuts at: 0 293 521 537 660 760 767 848 1072
Size: 293 228 16 123 100 7 81 224
Cuts at: 1072 1253 1869
Size: 181 616
Fragments arranged by size:
616 293 228 224 181 123 100 81
16 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1444 1869
Size: 1444 425
BbvCI CC'TCA_GC
Cuts at: 0 283 443 1869
Size: 283 160 1426
Fragments arranged by size:
1426 283 160
BclI T'GATC_A
Cuts at: 0 1595 1869
Size: 1595 274
BglI GCCn_nnn'nGGC
Cuts at: 0 721 1869
Size: 721 1148
Bme1580I G_kGCm'C
Cuts at: 0 440 660 760 848 899 1196 1514 1869
Size: 440 220 100 88 51 297 318 355
Fragments arranged by size:
440 355 318 297 220 100 88 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 1466 1738 1869
Size: 1466 272 131
Fragments arranged by size:
1466 272 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 947 1869
Size: 947 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 217 274 471 481 562 854 879
Size: 168 49 57 197 10 81 292 25
Cuts at: 879 894 1869
Size: 15 975
Fragments arranged by size:
975 292 197 168 81 57 49 25
15 10
Bpu10I CC'TnA_GC
Cuts at: 0 283 379 443 1869
Size: 283 96 64 1426
Fragments arranged by size:
1426 283 96 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1240 1869
Size: 1240 629
BsaI GGTCTCn'nnnn_
Cuts at: 0 538 1869
Size: 538 1331
BsaAI yAC'GTr
Cuts at: 0 706 1869
Size: 706 1163
BsaHI Gr'CG_yC
Cuts at: 0 163 321 386 1869
Size: 163 158 65 1483
Fragments arranged by size:
1483 163 158 65
BsaWI w'CCGG_w
Cuts at: 0 547 967 1869
Size: 547 420 902
Fragments arranged by size:
902 547 420
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 949 979 1672 1702 1744 1774
Size: 247 30 672 30 693 30 42 30
Cuts at: 1774 1869
Size: 95
Fragments arranged by size:
693 672 247 95 42 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 876 1222 1768 1869
Size: 876 346 546 101
Fragments arranged by size:
876 546 346 101
BseYI C'CCAG_C
Cuts at: 0 653 936 1195 1869
Size: 653 283 259 674
Fragments arranged by size:
674 653 283 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1447 1519 1869
Size: 1447 72 350
Fragments arranged by size:
1447 350 72
BsiHKAI G_wGCw'C
Cuts at: 0 293 521 767 899 1080 1226 1253 1653
Size: 293 228 246 132 181 146 27 400
Cuts at: 1653 1869
Size: 216
Fragments arranged by size:
400 293 246 228 216 181 146 132
27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 531 1869
Size: 531 1338
Bsp1286I G_dGCh'C
Cuts at: 0 293 440 521 537 660 760 767 848
Size: 293 147 81 16 123 100 7 81
Cuts at: 848 899 1072 1080 1196 1226 1253 1514 1653
Size: 51 173 8 116 30 27 261 139
Cuts at: 1653 1869
Size: 216
Fragments arranged by size:
293 261 216 173 147 139 123 116
100 81 81 51 30 27 16 8
7
BspEI T'CCGG_A
Cuts at: 0 967 1869
Size: 967 902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 1869
Size: 419 1450
BsrBI CCG'CTC
Cuts at: 0 201 1869
Size: 201 1668
BsrDI GCAATG_nn'
Cuts at: 0 1613 1869
Size: 1613 256
BsrFI r'CCGG_y
Cuts at: 0 547 1869
Size: 547 1322
BssHII G'CGCG_C
Cuts at: 0 451 1869
Size: 451 1418
BssSI C'ACGA_G
Cuts at: 0 921 1869
Size: 921 948
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1410 1869
Size: 1410 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1482 1551 1869
Size: 1482 69 318
Fragments arranged by size:
1482 318 69
BtsI GCAGTG_nn'
Cuts at: 0 811 956 1531 1653 1869
Size: 811 145 575 122 216
Fragments arranged by size:
811 575 216 145 122
DraIII CAC_nnn'GTG
Cuts at: 0 1329 1869
Size: 1329 540
EaeI y'GGCC_r
Cuts at: 0 206 356 564 713 779 1136 1175 1604
Size: 206 150 208 149 66 357 39 429
Cuts at: 1604 1869
Size: 265
Fragments arranged by size:
429 357 265 208 206 150 149 66
39
EarI CTCTTCn'nnn_
Cuts at: 0 1658 1869
Size: 1658 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1115 1869
Size: 1115 754
EcoICRI GAG'CTC
Cuts at: 0 291 519 765 1251 1869
Size: 291 228 246 486 618
Fragments arranged by size:
618 486 291 246 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 217 219 274 471 481 562 711
Size: 168 49 2 55 197 10 81 149
Cuts at: 711 854 879 894 1359 1639 1869
Size: 143 25 15 465 280 230
Fragments arranged by size:
465 280 230 197 168 149 143 81
55 49 25 15 10 2
EcoO109I rG'GnC_Cy
Cuts at: 0 239 312 582 657 756 791 878 1270
Size: 239 73 270 75 99 35 87 392
Cuts at: 1270 1503 1869
Size: 233 366
Fragments arranged by size:
392 366 270 239 233 99 87 75
73 35
HaeII r_GCGC'y
Cuts at: 0 379 1665 1869
Size: 379 1286 204
Fragments arranged by size:
1286 379 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 962 994 1436 1468 1744 1776 1869
Size: 962 32 442 32 276 32 93
Fragments arranged by size:
962 442 276 93 32 32 32
HincII GTy'rAC
Cuts at: 0 1716 1869
Size: 1716 153
MscI TGG'CCA
Cuts at: 0 781 1138 1177 1606 1869
Size: 781 357 39 429 263
Fragments arranged by size:
781 429 357 263 39
MspA1I CmG'CkG
Cuts at: 0 83 261 442 498 1168 1869
Size: 83 178 181 56 670 701
Fragments arranged by size:
701 670 181 178 83 56
NspI r_CATG'y
Cuts at: 0 21 614 1147 1413 1869
Size: 21 593 533 266 456
Fragments arranged by size:
593 533 456 266 21
PasI CC'CwG_GG
Cuts at: 0 243 368 605 841 1242 1869
Size: 243 125 237 236 401 627
Fragments arranged by size:
627 401 243 237 236 125
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 1869
Size: 99 1770
PfoI T'CCnGG_A
Cuts at: 0 458 771 1869
Size: 458 313 1098
Fragments arranged by size:
1098 458 313
PpuMI rG'GwC_Cy
Cuts at: 0 582 791 1270 1869
Size: 582 209 479 599
Fragments arranged by size:
599 582 479 209
PspOMI G'GGCC_C
Cuts at: 0 656 756 844 1869
Size: 656 100 88 1025
Fragments arranged by size:
1025 656 100 88
PstI C_TGCA'G
Cuts at: 0 88 416 951 1648 1869
Size: 88 328 535 697 221
Fragments arranged by size:
697 535 328 221 88
PvuII CAG'CTG
Cuts at: 0 83 261 498 1869
Size: 83 178 237 1371
Fragments arranged by size:
1371 237 178 83
SacI G_AGCT'C
Cuts at: 0 293 521 767 1253 1869
Size: 293 228 246 486 616
Fragments arranged by size:
616 486 293 246 228
SapI GCTCTTCn'nnn_
Cuts at: 0 1658 1869
Size: 1658 211
SbfI CC_TGCA'GG
Cuts at: 0 416 1869
Size: 416 1453
ScaI AGT'ACT
Cuts at: 0 1861 1869
Size: 1861 8
SexAI A'CCwGG_T
Cuts at: 0 632 1869
Size: 632 1237
SfcI C'TryA_G
Cuts at: 0 84 412 592 947 1644 1734 1869
Size: 84 328 180 355 697 90 135
Fragments arranged by size:
697 355 328 180 135 90 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 721 1869
Size: 721 1148
SmaI CCC'GGG
Cuts at: 0 58 345 1869
Size: 58 287 1524
Fragments arranged by size:
1524 287 58
SmlI C'TyrA_G
Cuts at: 0 1219 1869
Size: 1219 650
SphI G_CATG'C
Cuts at: 0 21 1413 1869
Size: 21 1392 456
Fragments arranged by size:
1392 456 21
StuI AGG'CCT
Cuts at: 0 232 1825 1869
Size: 232 1593 44
Fragments arranged by size:
1593 232 44
StyI C'CwwG_G
Cuts at: 0 585 1334 1869
Size: 585 749 535
Fragments arranged by size:
749 585 535
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 790 1869
Size: 91 699 1079
Fragments arranged by size:
1079 699 91
TatI w'GTAC_w
Cuts at: 0 1859 1869
Size: 1859 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 949 981 1175 1207 1315 1347
Size: 247 32 670 32 194 32 108 32
Cuts at: 1347 1869
Size: 522
Fragments arranged by size:
670 522 247 194 108 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 996 1079 1548 1551 1869
Size: 192 804 83 469 3 318
Fragments arranged by size:
804 469 318 192 83 3
XmaI C'CCGG_G
Cuts at: 0 56 343 1869
Size: 56 287 1526
Fragments arranged by size:
1526 287 56
Enzymes that do cut:
AcuI AfeI AgeI AlfI AlwNI ApaI ApaLI AvaI
AvrII BanI BanII BbsI BbvCI BclI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstXI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MscI MspA1I NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SbfI ScaI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AleI AloI ApoI AscI AseI AsiSI BaeI BamHI
BbeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
BsaBI BsiEI BsiWI BsmI BspHI BsrGI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI