Restriction map for
pF1KB7202
(Linear) (Six-base) MAPSORT of: KIBB7202.seq Check: 3830 from: 1 to: 723
KIBB7202.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 429 723
Size: 429 294
ApaI G_GGCC'C
Cuts at: 0 45 723
Size: 45 678
AvaI C'yCGr_G
Cuts at: 0 36 306 394 723
Size: 36 270 88 329
Fragments arranged by size:
329 270 88 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 252 285 723
Size: 252 33 438
Fragments arranged by size:
438 252 33
BanI G'GyrC_C
Cuts at: 0 138 500 723
Size: 138 362 223
Fragments arranged by size:
362 223 138
BanII G_rGCy'C
Cuts at: 0 45 287 563 590 723
Size: 45 242 276 27 133
Fragments arranged by size:
276 242 133 45 27
BbeI G_GCGC'C
Cuts at: 0 504 723
Size: 504 219
BbvCI CC'TCA_GC
Cuts at: 0 655 723
Size: 655 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 536 723
Size: 502 34 187
Fragments arranged by size:
502 187 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 280 428 723
Size: 280 148 295
Fragments arranged by size:
295 280 148
BglI GCCn_nnn'nGGC
Cuts at: 0 567 669 723
Size: 567 102 54
Fragments arranged by size:
567 102 54
Bme1580I G_kGCm'C
Cuts at: 0 45 141 313 723
Size: 45 96 172 410
Fragments arranged by size:
410 172 96 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 475 723
Size: 475 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 301 723
Size: 214 87 422
Fragments arranged by size:
422 214 87
Bpu10I CC'TnA_GC
Cuts at: 0 655 723
Size: 655 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 723
Size: 312 411
BsaAI yAC'GTr
Cuts at: 0 363 723
Size: 363 360
BsaHI Gr'CG_yC
Cuts at: 0 177 501 723
Size: 177 324 222
Fragments arranged by size:
324 222 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 207 237 723
Size: 207 30 486
Fragments arranged by size:
486 207 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 277 723
Size: 122 155 446
Fragments arranged by size:
446 155 122
BseYI C'CCAG_C
Cuts at: 0 128 142 366 422 441 448 723
Size: 128 14 224 56 19 7 275
Fragments arranged by size:
275 224 128 56 19 14 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 723
Size: 478 245
BsiEI CG_ry'CG
Cuts at: 0 75 184 246 723
Size: 75 109 62 477
Fragments arranged by size:
477 109 75 62
BsiHKAI G_wGCw'C
Cuts at: 0 287 461 723
Size: 287 174 262
Fragments arranged by size:
287 262 174
Bsp1286I G_dGCh'C
Cuts at: 0 45 141 287 313 461 563 590 723
Size: 45 96 146 26 148 102 27 133
Fragments arranged by size:
148 146 133 102 96 45 27 26
BsrBI CCG'CTC
Cuts at: 0 206 723
Size: 206 517
BsrFI r'CCGG_y
Cuts at: 0 668 723
Size: 668 55
BssSI C'ACGA_G
Cuts at: 0 334 723
Size: 334 389
BstYI r'GATC_y
Cuts at: 0 636 723
Size: 636 87
BtgI C'CryG_G
Cuts at: 0 405 723
Size: 405 318
EaeI y'GGCC_r
Cuts at: 0 72 243 723
Size: 72 171 480
Fragments arranged by size:
480 171 72
EagI C'GGCC_G
Cuts at: 0 72 243 723
Size: 72 171 480
Fragments arranged by size:
480 171 72
EarI CTCTTCn'nnn_
Cuts at: 0 204 578 723
Size: 204 374 145
Fragments arranged by size:
374 204 145
EcoICRI GAG'CTC
Cuts at: 0 285 723
Size: 285 438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 301 723
Size: 214 87 422
Fragments arranged by size:
422 214 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 723
Size: 610 113
EcoO109I rG'GnC_Cy
Cuts at: 0 41 162 209 377 390 603 662 723
Size: 41 121 47 168 13 213 59 61
Fragments arranged by size:
213 168 121 61 59 47 41 13
HaeII r_GCGC'y
Cuts at: 0 449 504 723
Size: 449 55 219
Fragments arranged by size:
449 219 55
KasI G'GCGC_C
Cuts at: 0 500 723
Size: 500 223
MspA1I CmG'CkG
Cuts at: 0 18 27 128 438 682 723
Size: 18 9 101 310 244 41
Fragments arranged by size:
310 244 101 41 18 9
NaeI GCC'GGC
Cuts at: 0 670 723
Size: 670 53
NarI GG'CG_CC
Cuts at: 0 501 723
Size: 501 222
NcoI C'CATG_G
Cuts at: 0 405 723
Size: 405 318
NgoMIV G'CCGG_C
Cuts at: 0 668 723
Size: 668 55
PasI CC'CwG_GG
Cuts at: 0 55 79 723
Size: 55 24 644
Fragments arranged by size:
644 55 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 128 723
Size: 128 595
PmlI CAC'GTG
Cuts at: 0 363 723
Size: 363 360
PpuMI rG'GwC_Cy
Cuts at: 0 209 377 603 723
Size: 209 168 226 120
Fragments arranged by size:
226 209 168 120
PspOMI G'GGCC_C
Cuts at: 0 41 723
Size: 41 682
PstI C_TGCA'G
Cuts at: 0 17 723
Size: 17 706
PvuII CAG'CTG
Cuts at: 0 18 723
Size: 18 705
SacI G_AGCT'C
Cuts at: 0 287 723
Size: 287 436
SanDI GG'GwC_CC
Cuts at: 0 209 723
Size: 209 514
SapI GCTCTTCn'nnn_
Cuts at: 0 578 723
Size: 578 145
SfcI C'TryA_G
Cuts at: 0 13 723
Size: 13 710
SfoI GGC'GCC
Cuts at: 0 502 723
Size: 502 221
SmaI CCC'GGG
Cuts at: 0 396 723
Size: 396 327
SmlI C'TyrA_G
Cuts at: 0 36 291 723
Size: 36 255 432
Fragments arranged by size:
432 255 36
StyI C'CwwG_G
Cuts at: 0 45 405 723
Size: 45 360 318
Fragments arranged by size:
360 318 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 198 723
Size: 156 42 525
Fragments arranged by size:
525 156 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 207 239 723
Size: 131 32 44 32 484
Fragments arranged by size:
484 131 44 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 52 723
Size: 52 671
XhoI C'TCGA_G
Cuts at: 0 36 723
Size: 36 687
XmaI C'CCGG_G
Cuts at: 0 394 723
Size: 394 329
Enzymes that do cut:
AlwNI ApaI AvaI BaeI BanI BanII BbeI BbvCI
BcgI BciVI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BssSI BstYI BtgI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PmlI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SfcI SfoI
SmaI SmlI StyI TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbsI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XmnI ZraI