(Linear) (Six-base) MAPSORT of: KIBB7192.seq  Check: 3873  from: 1  to: 951



KIBB7192.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     370     951 
   Size:        370     581
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     951 
   Size:        113     838
 
ApoI r'AATT_y

Cuts at:      0      60     951 
   Size:         60     891
 
BanI G'GyrC_C

Cuts at:      0     532     667     799     951 
   Size:        532     135     132     152

  Fragments arranged by size: 

                 532     152     135     132
 
BbeI G_GCGC'C

Cuts at:      0     803     951 
   Size:        803     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     299     641     682     951 
   Size:         10     289     342      41     269

  Fragments arranged by size: 

                 342     289     269      41      10
 
BlpI GC'TnA_GC

Cuts at:      0      22     951 
   Size:         22     929
 
Bme1580I G_kGCm'C

Cuts at:      0     670     951 
   Size:        670     281
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     343     951 
   Size:        343     608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     951 
   Size:        641     310
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     640     951 
   Size:        640     311
 
BsaHI Gr'CG_yC

Cuts at:      0     350     800     951 
   Size:        350     450     151

  Fragments arranged by size: 

                 450     350     151
 
BsaWI w'CCGG_w

Cuts at:      0     649     951 
   Size:        649     302
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     672     702     951 
   Size:        672      30     249

  Fragments arranged by size: 

                 672     249      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     247     259     475     596     951 
   Size:        244       3      12     216     121     355

  Fragments arranged by size: 

                 355     244     216     121      12       3
 
BseYI C'CCAG_C

Cuts at:      0     406     951 
   Size:        406     545
 
Bsp1286I G_dGCh'C

Cuts at:      0     670     951 
   Size:        670     281
 
BssSI C'ACGA_G

Cuts at:      0     854     951 
   Size:        854      97
 
BstZ17I GTA'TAC

Cuts at:      0     371     951 
   Size:        371     580
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     750     785     951 
   Size:        750      35     166

  Fragments arranged by size: 

                 750     166      35
 
DraI TTT'AAA

Cuts at:      0     164     900     951 
   Size:        164     736      51

  Fragments arranged by size: 

                 736     164      51
 
EarI CTCTTCn'nnn_

Cuts at:      0      66     221     236     452     461     951 
   Size:         66     155      15     216       9     490

  Fragments arranged by size: 

                 490     216     155      66      15       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     641     951 
   Size:        113     528     310

  Fragments arranged by size: 

                 528     310     113
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     362     951 
   Size:        362     589
 
HaeII r_GCGC'y

Cuts at:      0     803     951 
   Size:        803     148
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     812     844     951 
   Size:        280      32     500      32     107

  Fragments arranged by size: 

                 500     280     107      32      32
 
KasI G'GCGC_C

Cuts at:      0     799     951 
   Size:        799     152
 
MspA1I CmG'CkG

Cuts at:      0     578     951 
   Size:        578     373
 
NarI GG'CG_CC

Cuts at:      0     800     951 
   Size:        800     151
 
NspI r_CATG'y

Cuts at:      0     145     951 
   Size:        145     806
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     697     751     951 
   Size:        697      54     200

  Fragments arranged by size: 

                 697     200      54
 
SfcI C'TryA_G

Cuts at:      0     729     951 
   Size:        729     222
 
SfoI GGC'GCC

Cuts at:      0     801     951 
   Size:        801     150
 
SphI G_CATG'C

Cuts at:      0     145     951 
   Size:        145     806
 
TatI w'GTAC_w

Cuts at:      0     113     887     951 
   Size:        113     774      64

  Fragments arranged by size: 

                 774     113      64
 
XmnI GAAnn'nnTTC

Cuts at:      0     738     951 
   Size:        738     213
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BanI     BbeI     BbsI     BlpI Bme1580I
     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
 Bsp1286I    BssSI  BstZ17I    CspCI     DraI     EarI  Eco57MI    EcoNI
    HaeII    Hin4I     KasI   MspA1I     NarI     NspI    PflMI     SfcI
     SfoI     SphI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI