Restriction map for
pF1KB7191
(Linear) (Six-base) MAPSORT of: KIBB7191.seq Check: 8072 from: 1 to: 1623
KIBB7191.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AclI AA'CG_TT
Cuts at: 0 350 1623
Size: 350 1273
AfeI AGC'GCT
Cuts at: 0 972 1623
Size: 972 651
AflII C'TTAA_G
Cuts at: 0 1062 1623
Size: 1062 561
AhdI GACnn_n'nnGTC
Cuts at: 0 561 1623
Size: 561 1062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1012 1623
Size: 1012 611
AlwNI CAG_nnn'CTG
Cuts at: 0 298 646 1623
Size: 298 348 977
Fragments arranged by size:
977 348 298
ApoI r'AATT_y
Cuts at: 0 215 405 1623
Size: 215 190 1218
Fragments arranged by size:
1218 215 190
AseI AT'TA_AT
Cuts at: 0 694 1623
Size: 694 929
BanII G_rGCy'C
Cuts at: 0 1567 1623
Size: 1567 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 1623
Size: 516 1107
BbvCI CC'TCA_GC
Cuts at: 0 1549 1623
Size: 1549 74
BfrBI ATG'CAT
Cuts at: 0 732 1623
Size: 732 891
BglII A'GATC_T
Cuts at: 0 250 1556 1623
Size: 250 1306 67
Fragments arranged by size:
1306 250 67
BlpI GC'TnA_GC
Cuts at: 0 1566 1623
Size: 1566 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1467 1623
Size: 1467 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1581 1623
Size: 275 1306 42
Fragments arranged by size:
1306 275 42
Bpu10I CC'TnA_GC
Cuts at: 0 1549 1623
Size: 1549 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1623
Size: 499 1124
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 1392 1623
Size: 412 980 231
Fragments arranged by size:
980 412 231
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1623
Size: 538 1085
BseYI C'CCAG_C
Cuts at: 0 1342 1623
Size: 1342 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1028 1623
Size: 596 432 595
Fragments arranged by size:
596 595 432
BsiHKAI G_wGCw'C
Cuts at: 0 1567 1623
Size: 1567 56
Bsp1286I G_dGCh'C
Cuts at: 0 1567 1623
Size: 1567 56
BspHI T'CATG_A
Cuts at: 0 131 1623
Size: 131 1492
BsrGI T'GTAC_A
Cuts at: 0 150 1623
Size: 150 1473
BssSI C'ACGA_G
Cuts at: 0 994 1213 1623
Size: 994 219 410
Fragments arranged by size:
994 410 219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1308 1383 1623
Size: 1308 75 240
Fragments arranged by size:
1308 240 75
BstYI r'GATC_y
Cuts at: 0 250 1556 1623
Size: 250 1306 67
Fragments arranged by size:
1306 250 67
DraI TTT'AAA
Cuts at: 0 1140 1623
Size: 1140 483
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 1623
Size: 520 1103
EaeI y'GGCC_r
Cuts at: 0 656 1623
Size: 656 967
EcoICRI GAG'CTC
Cuts at: 0 1565 1623
Size: 1565 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1581 1623
Size: 275 1306 42
Fragments arranged by size:
1306 275 42
EcoO109I rG'GnC_Cy
Cuts at: 0 555 1485 1623
Size: 555 930 138
Fragments arranged by size:
930 555 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 260 831 1623
Size: 260 571 792
Fragments arranged by size:
792 571 260
HaeII r_GCGC'y
Cuts at: 0 974 1623
Size: 974 649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 1623
Size: 547 32 1044
Fragments arranged by size:
1044 547 32
HincII GTy'rAC
Cuts at: 0 36 1623
Size: 36 1587
MfeI C'AATT_G
Cuts at: 0 1463 1623
Size: 1463 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1490 1496 1623
Size: 404 1086 6 127
Fragments arranged by size:
1086 404 127 6
MscI TGG'CCA
Cuts at: 0 658 1623
Size: 658 965
MslI CAynn'nnrTG
Cuts at: 0 572 862 1623
Size: 572 290 761
Fragments arranged by size:
761 572 290
MspA1I CmG'CkG
Cuts at: 0 1012 1623
Size: 1012 611
NsiI A_TGCA'T
Cuts at: 0 734 1623
Size: 734 889
NspI r_CATG'y
Cuts at: 0 732 1373 1623
Size: 732 641 250
Fragments arranged by size:
732 641 250
PfoI T'CCnGG_A
Cuts at: 0 551 1623
Size: 551 1072
PpuMI rG'GwC_Cy
Cuts at: 0 555 1623
Size: 555 1068
PstI C_TGCA'G
Cuts at: 0 981 1623
Size: 981 642
PvuII CAG'CTG
Cuts at: 0 1012 1623
Size: 1012 611
SacI G_AGCT'C
Cuts at: 0 1567 1623
Size: 1567 56
SanDI GG'GwC_CC
Cuts at: 0 555 1623
Size: 555 1068
SfcI C'TryA_G
Cuts at: 0 977 1623
Size: 977 646
SmlI C'TyrA_G
Cuts at: 0 478 1062 1623
Size: 478 584 561
Fragments arranged by size:
584 561 478
SphI G_CATG'C
Cuts at: 0 1373 1623
Size: 1373 250
SspI AAT'ATT
Cuts at: 0 771 1503 1623
Size: 771 732 120
Fragments arranged by size:
771 732 120
StuI AGG'CCT
Cuts at: 0 1382 1623
Size: 1382 241
StyI C'CwwG_G
Cuts at: 0 1572 1623
Size: 1572 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 629 901 1432 1623
Size: 629 272 531 191
Fragments arranged by size:
629 531 272 191
TatI w'GTAC_w
Cuts at: 0 150 1467 1623
Size: 150 1317 156
Fragments arranged by size:
1317 156 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 1623
Size: 298 32 1293
Fragments arranged by size:
1293 298 32
Tth111I GACn'n_nGTC
Cuts at: 0 1268 1623
Size: 1268 355
XmnI GAAnn'nnTTC
Cuts at: 0 219 243 1623
Size: 219 24 1380
Fragments arranged by size:
1380 219 24
Enzymes that do cut:
AclI AfeI AflII AhdI AlfI AlwNI ApoI AseI
BanII BbsI BbvCI BfrBI BglII BlpI BplI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrGI BssSI BstXI BstYI DraI DrdI EaeI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII MfeI
MmeI MscI MslI MspA1I NsiI NspI PfoI PpuMI
PstI PvuII SacI SanDI SfcI SmlI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BglI Bme1580I
BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII EagI EarI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
XbaI XcmI XhoI XmaI ZraI