(Linear) (Six-base) MAPSORT of: KIBB7189.seq  Check: 1806  from: 1  to: 393



KIBB7189.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     393 
   Size:         27     366
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     393 
   Size:        105     288
 
ApaI G_GGCC'C

Cuts at:      0     218     393 
   Size:        218     175
 
AvaI C'yCGr_G

Cuts at:      0     219     331     393 
   Size:        219     112      62

  Fragments arranged by size: 

                 219     112      62
 
BanI G'GyrC_C

Cuts at:      0     335     393 
   Size:        335      58
 
BanII G_rGCy'C

Cuts at:      0     218     393 
   Size:        218     175
 
BbeI G_GCGC'C

Cuts at:      0     339     393 
   Size:        339      54
 
BglI GCCn_nnn'nGGC

Cuts at:      0     302     393 
   Size:        302      91
 
Bme1580I G_kGCm'C

Cuts at:      0     218     393 
   Size:        218     175
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     393 
   Size:        211     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      27     393 
   Size:         27     366
 
BsaHI Gr'CG_yC

Cuts at:      0     336     393 
   Size:        336      57
 
BseYI C'CCAG_C

Cuts at:      0     195     393 
   Size:        195     198
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     142     393 
   Size:         80      62     251

  Fragments arranged by size: 

                 251      80      62
 
BsiEI CG_ry'CG

Cuts at:      0     182     356     393 
   Size:        182     174      37

  Fragments arranged by size: 

                 182     174      37
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     393 
   Size:        218     175
 
BsrFI r'CCGG_y

Cuts at:      0     181     393 
   Size:        181     212
 
BtgI C'CryG_G

Cuts at:      0     272     287     393 
   Size:        272      15     106

  Fragments arranged by size: 

                 272     106      15
 
EaeI y'GGCC_r

Cuts at:      0     179     353     393 
   Size:        179     174      40

  Fragments arranged by size: 

                 179     174      40
 
EagI C'GGCC_G

Cuts at:      0     179     353     393 
   Size:        179     174      40

  Fragments arranged by size: 

                 179     174      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     336     393 
   Size:        336      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     393 
   Size:        211     182
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     250     393 
   Size:        250     143
 
EcoO109I rG'GnC_Cy

Cuts at:      0     215     393 
   Size:        215     178
 
HaeII r_GCGC'y

Cuts at:      0     246     319     339     393 
   Size:        246      73      20      54

  Fragments arranged by size: 

                 246      73      54      20
 
KasI G'GCGC_C

Cuts at:      0     335     393 
   Size:        335      58
 
MspA1I CmG'CkG

Cuts at:      0     260     274     341     393 
   Size:        260      14      67      52

  Fragments arranged by size: 

                 260      67      52      14
 
NaeI GCC'GGC

Cuts at:      0     183     393 
   Size:        183     210
 
NarI GG'CG_CC

Cuts at:      0     336     393 
   Size:        336      57
 
NcoI C'CATG_G

Cuts at:      0     287     393 
   Size:        287     106
 
NgoMIV G'CCGG_C

Cuts at:      0     181     393 
   Size:        181     212
 
PfoI T'CCnGG_A

Cuts at:      0     385     393 
   Size:        385       8
 
PshAI GACnn'nnGTC

Cuts at:      0     116     393 
   Size:        116     277
 
PspOMI G'GGCC_C

Cuts at:      0     214     393 
   Size:        214     179
 
PstI C_TGCA'G

Cuts at:      0     242     254     393 
   Size:        242      12     139

  Fragments arranged by size: 

                 242     139      12
 
SacII CC_GC'GG

Cuts at:      0     275     393 
   Size:        275     118
 
SbfI CC_TGCA'GG

Cuts at:      0     254     393 
   Size:        254     139
 
SfcI C'TryA_G

Cuts at:      0     238     250     393 
   Size:        238      12     143

  Fragments arranged by size: 

                 238     143      12
 
SfoI GGC'GCC

Cuts at:      0     337     393 
   Size:        337      56
 
SmaI CCC'GGG

Cuts at:      0     221     393 
   Size:        221     172
 
StyI C'CwwG_G

Cuts at:      0     287     393 
   Size:        287     106
 
XmaI C'CCGG_G

Cuts at:      0     219     393 
   Size:        219     174
 

 Enzymes that do cut:

     AloI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BglI
 Bme1580I     BpmI     BsaI    BsaHI    BseYI     BsgI    BsiEI Bsp1286I
    BsrFI     BtgI     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PfoI
    PshAI   PspOMI     PstI    SacII     SbfI     SfcI     SfoI     SmaI
     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI