Restriction map for
pF1KB7180
(Linear) (Six-base) MAPSORT of: KIBB7180.seq Check: 6318 from: 1 to: 1260
KIBB7180.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1225 1260
Size: 1225 35
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1260
Size: 659 601
AfeI AGC'GCT
Cuts at: 0 796 1260
Size: 796 464
AflIII A'CryG_T
Cuts at: 0 259 1260
Size: 259 1001
AlwNI CAG_nnn'CTG
Cuts at: 0 194 320 1260
Size: 194 126 940
Fragments arranged by size:
940 194 126
ApaI G_GGCC'C
Cuts at: 0 226 504 1038 1260
Size: 226 278 534 222
Fragments arranged by size:
534 278 226 222
AvaI C'yCGr_G
Cuts at: 0 214 225 1260
Size: 214 11 1035
Fragments arranged by size:
1035 214 11
BanI G'GyrC_C
Cuts at: 0 367 1044 1173 1260
Size: 367 677 129 87
Fragments arranged by size:
677 367 129 87
BanII G_rGCy'C
Cuts at: 0 226 280 504 1038 1079 1260
Size: 226 54 224 534 41 181
Fragments arranged by size:
534 226 224 181 54 41
BbeI G_GCGC'C
Cuts at: 0 1177 1260
Size: 1177 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 1260
Size: 583 677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1210 1244 1260
Size: 1210 34 16
Fragments arranged by size:
1210 34 16
BglI GCCn_nnn'nGGC
Cuts at: 0 1003 1022 1042 1171 1260
Size: 1003 19 20 129 89
Fragments arranged by size:
1003 129 89 20 19
BlpI GC'TnA_GC
Cuts at: 0 491 1260
Size: 491 769
Bme1580I G_kGCm'C
Cuts at: 0 226 504 988 1038 1260
Size: 226 278 484 50 222
Fragments arranged by size:
484 278 226 222 50
BmgBI CAC'GTC
Cuts at: 0 260 1260
Size: 260 1000
BmrI ACTGGGnnnn_n'
Cuts at: 0 1119 1260
Size: 1119 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1260
Size: 265 995
BsaHI Gr'CG_yC
Cuts at: 0 184 516 769 875 1174 1260
Size: 184 332 253 106 299 86
Fragments arranged by size:
332 299 253 184 106 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 895 1260
Size: 865 30 365
Fragments arranged by size:
865 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 934 1260
Size: 934 326
BseYI C'CCAG_C
Cuts at: 0 219 344 810 819 854 1062 1260
Size: 219 125 466 9 35 208 198
Fragments arranged by size:
466 219 208 198 125 35 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 1260
Size: 1197 63
BsiEI CG_ry'CG
Cuts at: 0 365 738 846 947 1026 1143 1260
Size: 365 373 108 101 79 117 117
Fragments arranged by size:
373 365 117 117 108 101 79
BsiHKAI G_wGCw'C
Cuts at: 0 314 1233 1260
Size: 314 919 27
Fragments arranged by size:
919 314 27
BsmI GAATG_Cn'
Cuts at: 0 1091 1260
Size: 1091 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 507 579 1260
Size: 507 72 681
Fragments arranged by size:
681 507 72
Bsp1286I G_dGCh'C
Cuts at: 0 226 280 314 504 988 1038 1079 1233
Size: 226 54 34 190 484 50 41 154
Cuts at: 1233 1260
Size: 27
Fragments arranged by size:
484 226 190 154 54 50 41 34
27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 563 1163 1225 1260
Size: 563 600 62 35
Fragments arranged by size:
600 563 62 35
BsrBI CCG'CTC
Cuts at: 0 1116 1145 1236 1260
Size: 1116 29 91 24
Fragments arranged by size:
1116 91 29 24
BsrFI r'CCGG_y
Cuts at: 0 357 364 841 1260
Size: 357 7 477 419
Fragments arranged by size:
477 419 357 7
BssHII G'CGCG_C
Cuts at: 0 145 1260
Size: 145 1115
BssSI C'ACGA_G
Cuts at: 0 1226 1260
Size: 1226 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 971 1260
Size: 971 289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1062 1177 1260
Size: 1062 115 83
Fragments arranged by size:
1062 115 83
BstYI r'GATC_y
Cuts at: 0 190 1260
Size: 190 1070
Bsu36I CC'TnA_GG
Cuts at: 0 427 1260
Size: 427 833
BtgI C'CryG_G
Cuts at: 0 203 539 1007 1017 1084 1260
Size: 203 336 468 10 67 176
Fragments arranged by size:
468 336 203 176 67 10
BtsI GCAGTG_nn'
Cuts at: 0 849 941 1260
Size: 849 92 319
Fragments arranged by size:
849 319 92
EaeI y'GGCC_r
Cuts at: 0 843 944 1023 1140 1260
Size: 843 101 79 117 120
Fragments arranged by size:
843 120 117 101 79
EagI C'GGCC_G
Cuts at: 0 843 944 1023 1140 1260
Size: 843 101 79 117 120
Fragments arranged by size:
843 120 117 101 79
EarI CTCTTCn'nnn_
Cuts at: 0 34 900 1260
Size: 34 866 360
Fragments arranged by size:
866 360 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 81 344 720 1260
Size: 81 263 376 540
Fragments arranged by size:
540 376 263 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 659 1260
Size: 265 394 601
Fragments arranged by size:
601 394 265
EcoO109I rG'GnC_Cy
Cuts at: 0 288 500 1035 1260
Size: 288 212 535 225
Fragments arranged by size:
535 288 225 212
FseI GG_CCGG'CC
Cuts at: 0 845 1260
Size: 845 415
FspI TGC'GCA
Cuts at: 0 827 1260
Size: 827 433
HaeII r_GCGC'y
Cuts at: 0 798 1177 1260
Size: 798 379 83
Fragments arranged by size:
798 379 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 35 46 67 249 281 581 613
Size: 14 21 11 21 182 32 300 32
Cuts at: 613 865 897 1129 1161 1260
Size: 252 32 232 32 99
Fragments arranged by size:
300 252 232 182 99 32 32 32
32 21 21 14 11
KasI G'GCGC_C
Cuts at: 0 1173 1260
Size: 1173 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1260
Size: 75 1185
MslI CAynn'nnrTG
Cuts at: 0 1096 1260
Size: 1096 164
MspA1I CmG'CkG
Cuts at: 0 252 344 814 1019 1086 1206 1252 1260
Size: 252 92 470 205 67 120 46 8
Fragments arranged by size:
470 252 205 120 92 67 46 8
NaeI GCC'GGC
Cuts at: 0 359 843 1260
Size: 359 484 417
Fragments arranged by size:
484 417 359
NarI GG'CG_CC
Cuts at: 0 1174 1260
Size: 1174 86
NcoI C'CATG_G
Cuts at: 0 203 539 1260
Size: 203 336 721
Fragments arranged by size:
721 336 203
NgoMIV G'CCGG_C
Cuts at: 0 357 841 1260
Size: 357 484 419
Fragments arranged by size:
484 419 357
NotI GC'GGCC_GC
Cuts at: 0 1023 1260
Size: 1023 237
PasI CC'CwG_GG
Cuts at: 0 700 1185 1260
Size: 700 485 75
Fragments arranged by size:
700 485 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 194 1260
Size: 194 1066
PpuMI rG'GwC_Cy
Cuts at: 0 288 1260
Size: 288 972
PspOMI G'GGCC_C
Cuts at: 0 222 500 1034 1260
Size: 222 278 534 226
Fragments arranged by size:
534 278 226 222
PstI C_TGCA'G
Cuts at: 0 889 1260
Size: 889 371
PvuII CAG'CTG
Cuts at: 0 252 814 1260
Size: 252 562 446
Fragments arranged by size:
562 446 252
SacII CC_GC'GG
Cuts at: 0 1020 1087 1260
Size: 1020 67 173
Fragments arranged by size:
1020 173 67
SfcI C'TryA_G
Cuts at: 0 885 1260
Size: 885 375
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1003 1022 1260
Size: 1003 19 238
Fragments arranged by size:
1003 238 19
SfoI GGC'GCC
Cuts at: 0 1175 1260
Size: 1175 85
SgrAI Cr'CCGG_yG
Cuts at: 0 357 1260
Size: 357 903
SmaI CCC'GGG
Cuts at: 0 227 1260
Size: 227 1033
StyI C'CwwG_G
Cuts at: 0 203 539 1260
Size: 203 336 721
Fragments arranged by size:
721 336 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 811 1260
Size: 811 449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 1260
Size: 375 32 853
Fragments arranged by size:
853 375 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 1260
Size: 546 714
XmaI C'CCGG_G
Cuts at: 0 225 1260
Size: 225 1035
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanI
BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I FseI FspI HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI PasI PflMI PpuMI PspOMI PstI PvuII
SacII SfcI SfiI SfoI SgrAI SmaI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NdeI NheI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI