Restriction map for
pF1KB7178
(Linear) (Six-base) MAPSORT of: KIBB7178.seq Check: 2623 from: 1 to: 1278
KIBB7178.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 309 1278
Size: 309 969
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1278
Size: 898 380
AflII C'TTAA_G
Cuts at: 0 411 1278
Size: 411 867
AleI CACnn'nnGTG
Cuts at: 0 889 1278
Size: 889 389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 1278
Size: 560 718
AlwNI CAG_nnn'CTG
Cuts at: 0 930 1278
Size: 930 348
ApaI G_GGCC'C
Cuts at: 0 657 1278
Size: 657 621
ApoI r'AATT_y
Cuts at: 0 57 1278
Size: 57 1221
AvaI C'yCGr_G
Cuts at: 0 740 1278
Size: 740 538
BanI G'GyrC_C
Cuts at: 0 7 823 1278
Size: 7 816 455
Fragments arranged by size:
816 455 7
BanII G_rGCy'C
Cuts at: 0 657 1278
Size: 657 621
BbeI G_GCGC'C
Cuts at: 0 827 1278
Size: 827 451
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 262 511 1278
Size: 209 53 249 767
Fragments arranged by size:
767 249 209 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 752 1278
Size: 718 34 526
Fragments arranged by size:
718 526 34
BglI GCCn_nnn'nGGC
Cuts at: 0 652 1278
Size: 652 626
BglII A'GATC_T
Cuts at: 0 914 1278
Size: 914 364
Bme1580I G_kGCm'C
Cuts at: 0 657 1278
Size: 657 621
BmrI ACTGGGnnnn_n'
Cuts at: 0 660 1107 1278
Size: 660 447 171
Fragments arranged by size:
660 447 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1278
Size: 53 1225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1278
Size: 85 1193
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 1278
Size: 619 659
BsaAI yAC'GTr
Cuts at: 0 336 582 795 873 1278
Size: 336 246 213 78 405
Fragments arranged by size:
405 336 246 213 78
BsaHI Gr'CG_yC
Cuts at: 0 529 824 1278
Size: 529 295 454
Fragments arranged by size:
529 454 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 661 691 1278
Size: 661 30 587
Fragments arranged by size:
661 587 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1278
Size: 538 740
BseYI C'CCAG_C
Cuts at: 0 905 1278
Size: 905 373
BsiHKAI G_wGCw'C
Cuts at: 0 692 800 1278
Size: 692 108 478
Fragments arranged by size:
692 478 108
BsmI GAATG_Cn'
Cuts at: 0 618 1278
Size: 618 660
BsmBI CGTCTCn'nnnn_
Cuts at: 0 520 1278
Size: 520 758
Bsp1286I G_dGCh'C
Cuts at: 0 657 692 800 1278
Size: 657 35 108 478
Fragments arranged by size:
657 478 108 35
BsrGI T'GTAC_A
Cuts at: 0 439 1278
Size: 439 839
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 1278
Size: 810 468
BstEII G'GTnAC_C
Cuts at: 0 882 1278
Size: 882 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 840 1278
Size: 474 366 438
Fragments arranged by size:
474 438 366
BstYI r'GATC_y
Cuts at: 0 371 914 1278
Size: 371 543 364
Fragments arranged by size:
543 371 364
BtgI C'CryG_G
Cuts at: 0 939 1278
Size: 939 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 1278
Size: 624 654
BtsI GCAGTG_nn'
Cuts at: 0 11 1278
Size: 11 1267
DraI TTT'AAA
Cuts at: 0 756 1278
Size: 756 522
DraIII CAC_nnn'GTG
Cuts at: 0 1058 1278
Size: 1058 220
EaeI y'GGCC_r
Cuts at: 0 255 350 635 1278
Size: 255 95 285 643
Fragments arranged by size:
643 285 255 95
EarI CTCTTCn'nnn_
Cuts at: 0 335 1059 1278
Size: 335 724 219
Fragments arranged by size:
724 335 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 898 1278
Size: 53 845 380
Fragments arranged by size:
845 380 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 1278
Size: 769 509
EcoO109I rG'GnC_Cy
Cuts at: 0 654 1278
Size: 654 624
EcoRV GAT'ATC
Cuts at: 0 846 1278
Size: 846 432
HaeII r_GCGC'y
Cuts at: 0 73 827 1278
Size: 73 754 451
Fragments arranged by size:
754 451 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1278
Size: 742 32 504
Fragments arranged by size:
742 504 32
KasI G'GCGC_C
Cuts at: 0 823 1278
Size: 823 455
MfeI C'AATT_G
Cuts at: 0 399 472 1010 1278
Size: 399 73 538 268
Fragments arranged by size:
538 399 268 73
MscI TGG'CCA
Cuts at: 0 257 352 637 1278
Size: 257 95 285 641
Fragments arranged by size:
641 285 257 95
MslI CAynn'nnrTG
Cuts at: 0 805 889 1278
Size: 805 84 389
Fragments arranged by size:
805 389 84
NarI GG'CG_CC
Cuts at: 0 824 1278
Size: 824 454
NcoI C'CATG_G
Cuts at: 0 939 1278
Size: 939 339
NspI r_CATG'y
Cuts at: 0 711 1278
Size: 711 567
PmlI CAC'GTG
Cuts at: 0 336 795 1278
Size: 336 459 483
Fragments arranged by size:
483 459 336
PsiI TTA'TAA
Cuts at: 0 179 1278
Size: 179 1099
PspOMI G'GGCC_C
Cuts at: 0 653 1278
Size: 653 625
SapI GCTCTTCn'nnn_
Cuts at: 0 1059 1278
Size: 1059 219
ScaI AGT'ACT
Cuts at: 0 1246 1278
Size: 1246 32
SexAI A'CCwGG_T
Cuts at: 0 695 1278
Size: 695 583
SfcI C'TryA_G
Cuts at: 0 306 1278
Size: 306 972
SfoI GGC'GCC
Cuts at: 0 825 1278
Size: 825 453
SmlI C'TyrA_G
Cuts at: 0 64 411 1278
Size: 64 347 867
Fragments arranged by size:
867 347 64
SphI G_CATG'C
Cuts at: 0 711 1278
Size: 711 567
StuI AGG'CCT
Cuts at: 0 233 988 1278
Size: 233 755 290
Fragments arranged by size:
755 290 233
StyI C'CwwG_G
Cuts at: 0 939 1141 1278
Size: 939 202 137
Fragments arranged by size:
939 202 137
TatI w'GTAC_w
Cuts at: 0 439 677 1244 1278
Size: 439 238 567 34
Fragments arranged by size:
567 439 238 34
XmnI GAAnn'nnTTC
Cuts at: 0 269 1278
Size: 269 1009
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BcgI BglI BglII
Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrGI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
KasI MfeI MscI MslI NarI NcoI NspI PmlI
PsiI PspOMI SapI ScaI SexAI SfcI SfoI SmlI
SphI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
Bpu10I BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstZ17I
Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MluI MmeI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI