Restriction map for
pF1KB7177
(Linear) (Six-base) MAPSORT of: KIBB7177.seq Check: 1103 from: 1 to: 1446
KIBB7177.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
ApaI G_GGCC'C
Cuts at: 0 16 1346 1446
Size: 16 1330 100
Fragments arranged by size:
1330 100 16
ApoI r'AATT_y
Cuts at: 0 682 1446
Size: 682 764
AvaI C'yCGr_G
Cuts at: 0 16 129 264 1446
Size: 16 113 135 1182
Fragments arranged by size:
1182 135 113 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 304 337 1446
Size: 304 33 1109
Fragments arranged by size:
1109 304 33
BanI G'GyrC_C
Cuts at: 0 133 219 333 1446
Size: 133 86 114 1113
Fragments arranged by size:
1113 133 114 86
BanII G_rGCy'C
Cuts at: 0 16 271 1346 1446
Size: 16 255 1075 100
Fragments arranged by size:
1075 255 100 16
BbeI G_GCGC'C
Cuts at: 0 137 223 1446
Size: 137 86 1223
Fragments arranged by size:
1223 137 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 1409 1446
Size: 709 700 37
Fragments arranged by size:
709 700 37
BbvCI CC'TCA_GC
Cuts at: 0 147 168 1446
Size: 147 21 1278
Fragments arranged by size:
1278 147 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 325 1446
Size: 291 34 1121
Fragments arranged by size:
1121 291 34
BclI T'GATC_A
Cuts at: 0 822 1446
Size: 822 624
BfrBI ATG'CAT
Cuts at: 0 1435 1446
Size: 1435 11
BglI GCCn_nnn'nGGC
Cuts at: 0 94 358 1446
Size: 94 264 1088
Fragments arranged by size:
1088 264 94
Bme1580I G_kGCm'C
Cuts at: 0 16 338 1346 1446
Size: 16 322 1008 100
Fragments arranged by size:
1008 322 100 16
BmgBI CAC'GTC
Cuts at: 0 1221 1446
Size: 1221 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 1340 1446
Size: 312 1028 106
Fragments arranged by size:
1028 312 106
Bpu10I CC'TnA_GC
Cuts at: 0 147 168 1446
Size: 147 21 1278
Fragments arranged by size:
1278 147 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1446
Size: 935 511
BsaI GGTCTCn'nnnn_
Cuts at: 0 27 1327 1446
Size: 27 1300 119
Fragments arranged by size:
1300 119 27
BsaBI GATnn'nnATC
Cuts at: 0 1434 1446
Size: 1434 12
BsaHI Gr'CG_yC
Cuts at: 0 134 220 1275 1446
Size: 134 86 1055 171
Fragments arranged by size:
1055 171 134 86
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 298 778 1446
Size: 135 163 480 668
Fragments arranged by size:
668 480 163 135
BsiEI CG_ry'CG
Cuts at: 0 56 98 158 1446
Size: 56 42 60 1288
Fragments arranged by size:
1288 60 56 42
BsiHKAI G_wGCw'C
Cuts at: 0 290 1074 1446
Size: 290 784 372
Fragments arranged by size:
784 372 290
Bsp1286I G_dGCh'C
Cuts at: 0 16 271 290 338 1074 1346 1446
Size: 16 255 19 48 736 272 100
Fragments arranged by size:
736 272 255 100 48 19 16
BsrBI CCG'CTC
Cuts at: 0 759 1446
Size: 759 687
BsrDI GCAATG_nn'
Cuts at: 0 446 452 1446
Size: 446 6 994
Fragments arranged by size:
994 446 6
BstEII G'GTnAC_C
Cuts at: 0 404 1446
Size: 404 1042
BstXI CCAn_nnnn'nTGG
Cuts at: 0 572 1446
Size: 572 874
BstYI r'GATC_y
Cuts at: 0 1043 1446
Size: 1043 403
Bsu36I CC'TnA_GG
Cuts at: 0 872 1446
Size: 872 574
BtgI C'CryG_G
Cuts at: 0 995 1446
Size: 995 451
BtsI GCAGTG_nn'
Cuts at: 0 119 983 1446
Size: 119 864 463
Fragments arranged by size:
864 463 119
EaeI y'GGCC_r
Cuts at: 0 53 95 155 192 1194 1446
Size: 53 42 60 37 1002 252
Fragments arranged by size:
1002 252 60 53 42 37
EagI C'GGCC_G
Cuts at: 0 53 95 155 1446
Size: 53 42 60 1291
Fragments arranged by size:
1291 60 53 42
EarI CTCTTCn'nnn_
Cuts at: 0 508 779 1151 1325 1391 1446
Size: 508 271 372 174 66 55
Fragments arranged by size:
508 372 271 174 66 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 51 57 72 1446
Size: 51 6 15 1374
Fragments arranged by size:
1374 51 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 932 1446
Size: 932 514
EcoO109I rG'GnC_Cy
Cuts at: 0 12 13 1446
Size: 12 1 1433
Fragments arranged by size:
1433 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1102 1180 1446
Size: 1102 78 266
Fragments arranged by size:
1102 266 78
HaeII r_GCGC'y
Cuts at: 0 137 223 1446
Size: 137 86 1223
Fragments arranged by size:
1223 137 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 855 887 1446
Size: 855 32 559
Fragments arranged by size:
855 559 32
KasI G'GCGC_C
Cuts at: 0 133 219 1446
Size: 133 86 1227
Fragments arranged by size:
1227 133 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 416 666 1126 1446
Size: 291 125 250 460 320
Fragments arranged by size:
460 320 291 250 125
MscI TGG'CCA
Cuts at: 0 194 1446
Size: 194 1252
MslI CAynn'nnrTG
Cuts at: 0 1387 1446
Size: 1387 59
NarI GG'CG_CC
Cuts at: 0 134 220 1446
Size: 134 86 1226
Fragments arranged by size:
1226 134 86
NotI GC'GGCC_GC
Cuts at: 0 53 1446
Size: 53 1393
NsiI A_TGCA'T
Cuts at: 0 1437 1446
Size: 1437 9
NspI r_CATG'y
Cuts at: 0 396 1446
Size: 396 1050
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1446
Size: 330 1116
PspOMI G'GGCC_C
Cuts at: 0 12 1342 1446
Size: 12 1330 104
Fragments arranged by size:
1330 104 12
SapI GCTCTTCn'nnn_
Cuts at: 0 508 1151 1446
Size: 508 643 295
Fragments arranged by size:
643 508 295
SfcI C'TryA_G
Cuts at: 0 665 1151 1446
Size: 665 486 295
Fragments arranged by size:
665 486 295
SfoI GGC'GCC
Cuts at: 0 135 221 1446
Size: 135 86 1225
Fragments arranged by size:
1225 135 86
SmlI C'TyrA_G
Cuts at: 0 950 1446
Size: 950 496
SpeI A'CTAG_T
Cuts at: 0 718 1446
Size: 718 728
SspI AAT'ATT
Cuts at: 0 1053 1446
Size: 1053 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 423 1446
Size: 423 1023
Tth111I GACn'n_nGTC
Cuts at: 0 368 1446
Size: 368 1078
Enzymes that do cut:
ApaI ApoI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI Bme1580I BmgBI BmrI
Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BsiEI BsiHKAI
Bsp1286I BsrBI BsrDI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI NarI NotI
NsiI NspI PflMI PspOMI SapI SfcI SfoI SmlI
SpeI SspI TaqII Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvrII BamHI BciVI BglII BlpI
BmtI BplI BpmI BsaAI BsaWI BsaXI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI Eco57MI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MspA1I NaeI NcoI NdeI NgoMIV NheI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI StyI SwaI
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI