Restriction map for
pF1KB7173
(Linear) (Six-base) MAPSORT of: KIBB7173.seq Check: 2823 from: 1 to: 1590
KIBB7173.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 273 1590
Size: 273 1317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 883 1408 1590
Size: 232 651 525 182
Fragments arranged by size:
651 525 232 182
AhdI GACnn_n'nnGTC
Cuts at: 0 12 1590
Size: 12 1578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 1590
Size: 106 32 1452
Fragments arranged by size:
1452 106 32
AlwNI CAG_nnn'CTG
Cuts at: 0 240 504 1590
Size: 240 264 1086
Fragments arranged by size:
1086 264 240
ApaI G_GGCC'C
Cuts at: 0 355 599 1590
Size: 355 244 991
Fragments arranged by size:
991 355 244
ApoI r'AATT_y
Cuts at: 0 567 1148 1590
Size: 567 581 442
Fragments arranged by size:
581 567 442
AvaI C'yCGr_G
Cuts at: 0 247 484 592 1590
Size: 247 237 108 998
Fragments arranged by size:
998 247 237 108
BamHI G'GATC_C
Cuts at: 0 515 1590
Size: 515 1075
BanI G'GyrC_C
Cuts at: 0 693 753 832 1590
Size: 693 60 79 758
Fragments arranged by size:
758 693 79 60
BanII G_rGCy'C
Cuts at: 0 259 311 355 539 599 1478 1590
Size: 259 52 44 184 60 879 112
Fragments arranged by size:
879 259 184 112 60 52 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1418 1590
Size: 1418 172
BciVI GTATCCnnnnn_n'
Cuts at: 0 710 1590
Size: 710 880
BclI T'GATC_A
Cuts at: 0 95 1590
Size: 95 1495
BlpI GC'TnA_GC
Cuts at: 0 89 904 1234 1590
Size: 89 815 330 356
Fragments arranged by size:
815 356 330 89
Bme1580I G_kGCm'C
Cuts at: 0 355 589 599 638 974 1590
Size: 355 234 10 39 336 616
Fragments arranged by size:
616 355 336 234 39 10
BmgBI CAC'GTC
Cuts at: 0 387 1169 1590
Size: 387 782 421
Fragments arranged by size:
782 421 387
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 1130 1590
Size: 253 877 460
Fragments arranged by size:
877 460 253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 1590
Size: 312 1278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 397 426 547 768 865 1590
Size: 94 303 29 121 221 97 725
Fragments arranged by size:
725 303 221 121 97 94 29
Bpu10I CC'TnA_GC
Cuts at: 0 1107 1590
Size: 1107 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1590
Size: 964 626
BsaWI w'CCGG_w
Cuts at: 0 1049 1499 1590
Size: 1049 450 91
Fragments arranged by size:
1049 450 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 388 418 1529 1559 1590
Size: 106 30 252 30 1111 30 31
Fragments arranged by size:
1111 252 106 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 429 605 1590
Size: 429 176 985
Fragments arranged by size:
985 429 176
BseYI C'CCAG_C
Cuts at: 0 491 497 520 1182 1252 1590
Size: 491 6 23 662 70 338
Fragments arranged by size:
662 491 338 70 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1590
Size: 802 788
BsiHKAI G_wGCw'C
Cuts at: 0 311 563 1590
Size: 311 252 1027
Fragments arranged by size:
1027 311 252
Bsp1286I G_dGCh'C
Cuts at: 0 259 311 355 539 563 589 599 638
Size: 259 52 44 184 24 26 10 39
Cuts at: 638 974 1478 1590
Size: 336 504 112
Fragments arranged by size:
504 336 259 184 112 52 44 39
26 24 10
BspHI T'CATG_A
Cuts at: 0 338 1590
Size: 338 1252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 273 1590
Size: 17 256 1317
Fragments arranged by size:
1317 256 17
BsrBI CCG'CTC
Cuts at: 0 475 1590
Size: 475 1115
BsrDI GCAATG_nn'
Cuts at: 0 330 1590
Size: 330 1260
BstEII G'GTnAC_C
Cuts at: 0 150 1590
Size: 150 1440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 690 1143 1590
Size: 132 558 453 447
Fragments arranged by size:
558 453 447 132
BstYI r'GATC_y
Cuts at: 0 515 951 1590
Size: 515 436 639
Fragments arranged by size:
639 515 436
Bsu36I CC'TnA_GG
Cuts at: 0 511 887 1480 1590
Size: 511 376 593 110
Fragments arranged by size:
593 511 376 110
BtgI C'CryG_G
Cuts at: 0 29 368 1460 1590
Size: 29 339 1092 130
Fragments arranged by size:
1092 339 130 29
BtsI GCAGTG_nn'
Cuts at: 0 1218 1590
Size: 1218 372
DraI TTT'AAA
Cuts at: 0 828 1590
Size: 828 762
DraIII CAC_nnn'GTG
Cuts at: 0 1119 1590
Size: 1119 471
EaeI y'GGCC_r
Cuts at: 0 221 1307 1568 1590
Size: 221 1086 261 22
Fragments arranged by size:
1086 261 221 22
EarI CTCTTCn'nnn_
Cuts at: 0 469 1448 1590
Size: 469 979 142
Fragments arranged by size:
979 469 142
EcoICRI GAG'CTC
Cuts at: 0 309 1590
Size: 309 1281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 232 397 426 547 768 865 883
Size: 94 138 165 29 121 221 97 18
Cuts at: 883 1408 1590
Size: 525 182
Fragments arranged by size:
525 221 182 165 138 121 97 94
29 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 466 1590
Size: 421 45 1124
Fragments arranged by size:
1124 421 45
EcoO109I rG'GnC_Cy
Cuts at: 0 144 351 352 429 480 596 945 1179
Size: 144 207 1 77 51 116 349 234
Cuts at: 1179 1590
Size: 411
Fragments arranged by size:
411 349 234 207 144 116 77 51
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 912 1590
Size: 912 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1182 1214 1590
Size: 30 1152 32 376
Fragments arranged by size:
1152 376 32 30
MscI TGG'CCA
Cuts at: 0 1570 1590
Size: 1570 20
MslI CAynn'nnrTG
Cuts at: 0 34 277 283 751 925 1120 1285 1590
Size: 34 243 6 468 174 195 165 305
Fragments arranged by size:
468 305 243 195 174 165 34 6
MspA1I CmG'CkG
Cuts at: 0 93 1256 1590
Size: 93 1163 334
Fragments arranged by size:
1163 334 93
NcoI C'CATG_G
Cuts at: 0 29 1460 1590
Size: 29 1431 130
Fragments arranged by size:
1431 130 29
NspI r_CATG'y
Cuts at: 0 282 1545 1590
Size: 282 1263 45
Fragments arranged by size:
1263 282 45
PasI CC'CwG_GG
Cuts at: 0 348 582 1590
Size: 348 234 1008
Fragments arranged by size:
1008 348 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 1590
Size: 504 1086
PfoI T'CCnGG_A
Cuts at: 0 503 1010 1590
Size: 503 507 580
Fragments arranged by size:
580 507 503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1590
Size: 106 32 1452
Fragments arranged by size:
1452 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 480 1590
Size: 480 1110
PspOMI G'GGCC_C
Cuts at: 0 351 595 1590
Size: 351 244 995
Fragments arranged by size:
995 351 244
PstI C_TGCA'G
Cuts at: 0 14 1590
Size: 14 1576
PvuII CAG'CTG
Cuts at: 0 93 1590
Size: 93 1497
SacI G_AGCT'C
Cuts at: 0 311 1590
Size: 311 1279
ScaI AGT'ACT
Cuts at: 0 895 1590
Size: 895 695
SexAI A'CCwGG_T
Cuts at: 0 131 1590
Size: 131 1459
SfcI C'TryA_G
Cuts at: 0 10 1590
Size: 10 1580
SmlI C'TyrA_G
Cuts at: 0 979 1590
Size: 979 611
StuI AGG'CCT
Cuts at: 0 109 1370 1590
Size: 109 1261 220
Fragments arranged by size:
1261 220 109
StyI C'CwwG_G
Cuts at: 0 29 56 1310 1446 1460 1590
Size: 29 27 1254 136 14 130
Fragments arranged by size:
1254 136 130 29 27 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 850 1590
Size: 361 489 740
Fragments arranged by size:
740 489 361
TatI w'GTAC_w
Cuts at: 0 893 1590
Size: 893 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1039 1071 1563 1590
Size: 388 32 619 32 492 27
Fragments arranged by size:
619 492 388 32 32 27
Enzymes that do cut:
AarI AcuI AhdI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BciVI BclI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BfrBI BglI BglII BmtI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI