Restriction map for
pF1KB7172
(Linear) (Six-base) MAPSORT of: KIBB7172.seq Check: 3166 from: 1 to: 1473
KIBB7172.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 815 1473
Size: 815 658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1473
Size: 167 1306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 1473
Size: 115 1358
ApoI r'AATT_y
Cuts at: 0 240 745 896 1473
Size: 240 505 151 577
Fragments arranged by size:
577 505 240 151
BanI G'GyrC_C
Cuts at: 0 940 1473
Size: 940 533
BanII G_rGCy'C
Cuts at: 0 332 1473
Size: 332 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 1427 1473
Size: 1427 46
BfrBI ATG'CAT
Cuts at: 0 1169 1473
Size: 1169 304
BglI GCCn_nnn'nGGC
Cuts at: 0 808 1473
Size: 808 665
BlpI GC'TnA_GC
Cuts at: 0 551 1473
Size: 551 922
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 324 405 1473
Size: 130 194 81 1068
Fragments arranged by size:
1068 194 130 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 487 1473
Size: 181 306 986
Fragments arranged by size:
986 306 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1473
Size: 594 879
BsaI GGTCTCn'nnnn_
Cuts at: 0 938 1473
Size: 938 535
BsaBI GATnn'nnATC
Cuts at: 0 212 1473
Size: 212 1261
BsaWI w'CCGG_w
Cuts at: 0 500 657 1473
Size: 500 157 816
Fragments arranged by size:
816 500 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 325 667 697 1432 1462 1473
Size: 265 30 30 342 30 735 30 11
Fragments arranged by size:
735 342 265 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 420 558 1473
Size: 289 131 138 915
Fragments arranged by size:
915 289 138 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1290 1473
Size: 1290 183
BsiHKAI G_wGCw'C
Cuts at: 0 332 1473
Size: 332 1141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 452 1473
Size: 452 1021
Bsp1286I G_dGCh'C
Cuts at: 0 332 1473
Size: 332 1141
BspEI T'CCGG_A
Cuts at: 0 500 1473
Size: 500 973
BspHI T'CATG_A
Cuts at: 0 1296 1473
Size: 1296 177
BsrBI CCG'CTC
Cuts at: 0 730 1473
Size: 730 743
BsrDI GCAATG_nn'
Cuts at: 0 686 1473
Size: 686 787
BssSI C'ACGA_G
Cuts at: 0 385 1473
Size: 385 1088
BstEII G'GTnAC_C
Cuts at: 0 1134 1473
Size: 1134 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 28 970 1452 1473
Size: 28 942 482 21
Fragments arranged by size:
942 482 28 21
BstYI r'GATC_y
Cuts at: 0 869 1473
Size: 869 604
BtgI C'CryG_G
Cuts at: 0 995 1031 1473
Size: 995 36 442
Fragments arranged by size:
995 442 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7 1140 1473
Size: 7 1133 333
Fragments arranged by size:
1133 333 7
BtsI GCAGTG_nn'
Cuts at: 0 481 1278 1473
Size: 481 797 195
Fragments arranged by size:
797 481 195
EaeI y'GGCC_r
Cuts at: 0 185 599 1473
Size: 185 414 874
Fragments arranged by size:
874 414 185
EarI CTCTTCn'nnn_
Cuts at: 0 143 337 434 824 991 1371 1473
Size: 143 194 97 390 167 380 102
Fragments arranged by size:
390 380 194 167 143 102 97
EcoICRI GAG'CTC
Cuts at: 0 330 1473
Size: 330 1143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 181 487 1473
Size: 167 14 306 986
Fragments arranged by size:
986 306 167 14
EcoRI G'AATT_C
Cuts at: 0 896 1473
Size: 896 577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 991 1473
Size: 991 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 766 798 1036 1068 1381 1413
Size: 662 32 72 32 238 32 313 32
Cuts at: 1413 1473
Size: 60
Fragments arranged by size:
662 313 238 72 60 32 32 32
32
HincII GTy'rAC
Cuts at: 0 51 84 1473
Size: 51 33 1389
Fragments arranged by size:
1389 51 33
HindIII A'AGCT_T
Cuts at: 0 553 904 1473
Size: 553 351 569
Fragments arranged by size:
569 553 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1473
Size: 602 871
MscI TGG'CCA
Cuts at: 0 601 1473
Size: 601 872
MslI CAynn'nnrTG
Cuts at: 0 1099 1186 1258 1381 1473
Size: 1099 87 72 123 92
Fragments arranged by size:
1099 123 92 87 72
MspA1I CmG'CkG
Cuts at: 0 1338 1473
Size: 1338 135
NsiI A_TGCA'T
Cuts at: 0 1171 1473
Size: 1171 302
NspI r_CATG'y
Cuts at: 0 268 621 1169 1473
Size: 268 353 548 304
Fragments arranged by size:
548 353 304 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 1079 1473
Size: 1079 394
PshAI GACnn'nnGTC
Cuts at: 0 930 1473
Size: 930 543
PstI C_TGCA'G
Cuts at: 0 352 1473
Size: 352 1121
SacI G_AGCT'C
Cuts at: 0 332 1473
Size: 332 1141
SapI GCTCTTCn'nnn_
Cuts at: 0 337 1473
Size: 337 1136
ScaI AGT'ACT
Cuts at: 0 313 1234 1473
Size: 313 921 239
Fragments arranged by size:
921 313 239
SfcI C'TryA_G
Cuts at: 0 348 812 1370 1473
Size: 348 464 558 103
Fragments arranged by size:
558 464 348 103
SmlI C'TyrA_G
Cuts at: 0 573 1473
Size: 573 900
StyI C'CwwG_G
Cuts at: 0 821 1139 1451 1473
Size: 821 318 312 22
Fragments arranged by size:
821 318 312 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 920 1141 1473
Size: 920 221 332
Fragments arranged by size:
920 332 221
TatI w'GTAC_w
Cuts at: 0 311 1232 1473
Size: 311 921 241
Fragments arranged by size:
921 311 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 667 699 1473
Size: 265 32 370 32 774
Fragments arranged by size:
774 370 265 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 778 1473
Size: 778 695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 637 1473
Size: 637 836
Enzymes that do cut:
AccI AcuI AlfI ApoI BanI BanII BbsI BfrBI
BglI BlpI BmrI BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrDI BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EarI EcoICRI Eco57MI EcoRI FalI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NsiI NspI
PflMI PshAI PstI SacI SapI ScaI SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaHI BseYI BsiEI BsiWI BsmI BspMI BsrFI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI XmnI ZraI