Restriction map for
pF1KB7169
(Linear) (Six-base) MAPSORT of: KIBB7169.seq Check: 9232 from: 1 to: 1299
KIBB7169.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1299
Size: 706 593
AlwNI CAG_nnn'CTG
Cuts at: 0 71 729 1299
Size: 71 658 570
Fragments arranged by size:
658 570 71
ApaLI G'TGCA_C
Cuts at: 0 289 1299
Size: 289 1010
AvaI C'yCGr_G
Cuts at: 0 938 1030 1299
Size: 938 92 269
Fragments arranged by size:
938 269 92
BamHI G'GATC_C
Cuts at: 0 98 527 692 1299
Size: 98 429 165 607
Fragments arranged by size:
607 429 165 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 1204 1299
Size: 1204 95
BbvCI CC'TCA_GC
Cuts at: 0 442 1299
Size: 442 857
BglI GCCn_nnn'nGGC
Cuts at: 0 680 1299
Size: 680 619
BlpI GC'TnA_GC
Cuts at: 0 1255 1299
Size: 1255 44
Bme1580I G_kGCm'C
Cuts at: 0 284 293 984 1299
Size: 284 9 691 315
Fragments arranged by size:
691 315 284 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 91 1299
Size: 91 1208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 448 1299
Size: 30 418 851
Fragments arranged by size:
851 418 30
Bpu10I CC'TnA_GC
Cuts at: 0 442 971 1246 1299
Size: 442 529 275 53
Fragments arranged by size:
529 442 275 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 1111 1299
Size: 856 255 188
Fragments arranged by size:
856 255 188
BsaAI yAC'GTr
Cuts at: 0 276 1299
Size: 276 1023
BsaBI GATnn'nnATC
Cuts at: 0 103 1299
Size: 103 1196
BsaWI w'CCGG_w
Cuts at: 0 95 524 695 1299
Size: 95 429 171 604
Fragments arranged by size:
604 429 171 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1156 1186 1299
Size: 1156 30 113
Fragments arranged by size:
1156 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 322 349 762 916 1030 1090 1299
Size: 49 273 27 413 154 114 60 209
Fragments arranged by size:
413 273 209 154 114 60 49 27
BseYI C'CCAG_C
Cuts at: 0 167 339 1299
Size: 167 172 960
Fragments arranged by size:
960 172 167
BsiEI CG_ry'CG
Cuts at: 0 119 1299
Size: 119 1180
BsiHKAI G_wGCw'C
Cuts at: 0 50 293 1299
Size: 50 243 1006
Fragments arranged by size:
1006 243 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 258 1299
Size: 258 1041
Bsp1286I G_dGCh'C
Cuts at: 0 50 284 293 984 1299
Size: 50 234 9 691 315
Fragments arranged by size:
691 315 234 50 9
BspEI T'CCGG_A
Cuts at: 0 95 524 1299
Size: 95 429 775
Fragments arranged by size:
775 429 95
BsrDI GCAATG_nn'
Cuts at: 0 761 1299
Size: 761 538
BsrFI r'CCGG_y
Cuts at: 0 551 1299
Size: 551 748
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 1185 1299
Size: 288 897 114
Fragments arranged by size:
897 288 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 573 1299
Size: 175 398 726
Fragments arranged by size:
726 398 175
BstYI r'GATC_y
Cuts at: 0 98 527 692 1299
Size: 98 429 165 607
Fragments arranged by size:
607 429 165 98
BtgI C'CryG_G
Cuts at: 0 596 1070 1299
Size: 596 474 229
Fragments arranged by size:
596 474 229
BtsI GCAGTG_nn'
Cuts at: 0 871 955 1299
Size: 871 84 344
Fragments arranged by size:
871 344 84
DraIII CAC_nnn'GTG
Cuts at: 0 288 1299
Size: 288 1011
EaeI y'GGCC_r
Cuts at: 0 116 1091 1299
Size: 116 975 208
Fragments arranged by size:
975 208 116
EagI C'GGCC_G
Cuts at: 0 116 1299
Size: 116 1183
EarI CTCTTCn'nnn_
Cuts at: 0 385 703 806 1145 1299
Size: 385 318 103 339 154
Fragments arranged by size:
385 339 318 154 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 1299
Size: 346 953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 448 706 1299
Size: 30 418 258 593
Fragments arranged by size:
593 418 258 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 708 1299
Size: 708 591
EcoO109I rG'GnC_Cy
Cuts at: 0 434 446 919 1299
Size: 434 12 473 380
Fragments arranged by size:
473 434 380 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1299
Size: 495 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 519 551 1144 1156 1176 1188
Size: 130 32 357 32 593 12 20 12
Cuts at: 1188 1299
Size: 111
Fragments arranged by size:
593 357 130 111 32 32 20 12
12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1142 1299
Size: 173 969 157
Fragments arranged by size:
969 173 157
MspA1I CmG'CkG
Cuts at: 0 398 545 1192 1299
Size: 398 147 647 107
Fragments arranged by size:
647 398 147 107
PasI CC'CwG_GG
Cuts at: 0 729 924 1299
Size: 729 195 375
Fragments arranged by size:
729 375 195
PpuMI rG'GwC_Cy
Cuts at: 0 434 446 919 1299
Size: 434 12 473 380
Fragments arranged by size:
473 434 380 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 178 210 1299
Size: 178 32 1089
Fragments arranged by size:
1089 178 32
PstI C_TGCA'G
Cuts at: 0 242 550 1014 1299
Size: 242 308 464 285
Fragments arranged by size:
464 308 285 242
PvuII CAG'CTG
Cuts at: 0 1192 1299
Size: 1192 107
SanDI GG'GwC_CC
Cuts at: 0 434 1299
Size: 434 865
SapI GCTCTTCn'nnn_
Cuts at: 0 385 1145 1299
Size: 385 760 154
Fragments arranged by size:
760 385 154
SfcI C'TryA_G
Cuts at: 0 238 546 716 1010 1299
Size: 238 308 170 294 289
Fragments arranged by size:
308 294 289 238 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 680 1299
Size: 680 619
SmaI CCC'GGG
Cuts at: 0 940 1299
Size: 940 359
StuI AGG'CCT
Cuts at: 0 205 714 1299
Size: 205 509 585
Fragments arranged by size:
585 509 205
StyI C'CwwG_G
Cuts at: 0 108 788 1299
Size: 108 680 511
Fragments arranged by size:
680 511 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 222 859 1299
Size: 181 41 637 440
Fragments arranged by size:
637 440 181 41
XmaI C'CCGG_G
Cuts at: 0 938 1299
Size: 938 361
Enzymes that do cut:
AcuI AlwNI ApaLI AvaI BamHI BbsI BbvCI BglI
BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI Hin4I MmeI MspA1I PasI PpuMI PsrI PstI
PvuII SanDI SapI SfcI SfiI SmaI StuI StyI
TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BanI BanII BbeI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpuEI
BsaHI BsgI BsiWI BsmI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI