(Linear) (Six-base) MAPSORT of: KIBB7168.seq  Check: 2755  from: 1  to: 1236



KIBB7168.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     821    1236 
   Size:        821     415
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     597    1236 
   Size:        597     639
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     914     946    1236 
   Size:        914      32     290

  Fragments arranged by size: 

                 914     290      32
 
ApoI r'AATT_y

Cuts at:      0     138    1021    1188    1236 
   Size:        138     883     167      48

  Fragments arranged by size: 

                 883     167     138      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245     603     737    1236 
   Size:        245     358     134     499

  Fragments arranged by size: 

                 499     358     245     134
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     210     481    1236 
   Size:        210     271     755

  Fragments arranged by size: 

                 755     271     210
 
BclI T'GATC_A

Cuts at:      0     248    1236 
   Size:        248     988
 
BglI GCCn_nnn'nGGC

Cuts at:      0     625    1236 
   Size:        625     611
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476     890    1236 
   Size:        476     414     346

  Fragments arranged by size: 

                 476     414     346
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     972    1203    1236 
   Size:        972     231      33

  Fragments arranged by size: 

                 972     231      33
 
BsaBI GATnn'nnATC

Cuts at:      0     412    1236 
   Size:        412     824
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     210     240    1236 
   Size:        210      30     996

  Fragments arranged by size: 

                 996     210      30
 
BseYI C'CCAG_C

Cuts at:      0      92     571    1236 
   Size:         92     479     665

  Fragments arranged by size: 

                 665     479      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     842    1081    1236 
   Size:        842     239     155

  Fragments arranged by size: 

                 842     239     155
 
BsmI GAATG_Cn'

Cuts at:      0     991    1236 
   Size:        991     245
 
BspHI T'CATG_A

Cuts at:      0     707    1236 
   Size:        707     529
 
BsrDI GCAATG_nn'

Cuts at:      0     528     582     962    1236 
   Size:        528      54     380     274

  Fragments arranged by size: 

                 528     380     274      54
 
BssSI C'ACGA_G

Cuts at:      0    1090    1236 
   Size:       1090     146
 
BstYI r'GATC_y

Cuts at:      0     328     826    1236 
   Size:        328     498     410

  Fragments arranged by size: 

                 498     410     328
 
BstZ17I GTA'TAC

Cuts at:      0     822    1236 
   Size:        822     414
 
BtgI C'CryG_G

Cuts at:      0     212    1236 
   Size:        212    1024
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     116    1236 
   Size:        116    1120
 
BtsI GCAGTG_nn'

Cuts at:      0     800    1236 
   Size:        800     436
 
DraIII CAC_nnn'GTG

Cuts at:      0     590    1236 
   Size:        590     646
 
EarI CTCTTCn'nnn_

Cuts at:      0     508    1236 
   Size:        508     728
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     597     972    1203    1236 
   Size:        597     375     231      33

  Fragments arranged by size: 

                 597     375     231      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     468    1236 
   Size:        468     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     485     517     789     821    1121    1153    1236 
   Size:        485      32     272      32     300      32      83

  Fragments arranged by size: 

                 485     300     272      83      32      32      32
 
HincII GTy'rAC

Cuts at:      0     663    1236 
   Size:        663     573
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1236 
   Size:        856     380
 
MslI CAynn'nnrTG

Cuts at:      0      64     347     781    1236 
   Size:         64     283     434     455

  Fragments arranged by size: 

                 455     434     283      64
 
NcoI C'CATG_G

Cuts at:      0     212    1236 
   Size:        212    1024
 
NspI r_CATG'y

Cuts at:      0     852    1236 
   Size:        852     384
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     590     940    1236 
   Size:        590     350     296

  Fragments arranged by size: 

                 590     350     296
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     201     233    1236 
   Size:        201      32    1003

  Fragments arranged by size: 

                1003     201      32
 
PstI C_TGCA'G

Cuts at:      0     990    1236 
   Size:        990     246
 
SfcI C'TryA_G

Cuts at:      0     605     671     986    1236 
   Size:        605      66     315     250

  Fragments arranged by size: 

                 605     315     250      66
 
StyI C'CwwG_G

Cuts at:      0     212    1013    1236 
   Size:        212     801     223

  Fragments arranged by size: 

                 801     223     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     371    1236 
   Size:        371     865
 
TatI w'GTAC_w

Cuts at:      0    1047    1236 
   Size:       1047     189
 
XbaI T'CTAG_A

Cuts at:      0     399    1236 
   Size:        399     837
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63     783    1236 
   Size:         63     720     453

  Fragments arranged by size: 

                 720     453      63
 

 Enzymes that do cut:

     AccI     AcuI     AloI     ApoI     BbsI    BciVI     BclI     BglI
     BmrI     BpmI    BsaBI    BsaXI    BseYI     BsgI     BsmI    BspHI
    BsrDI    BssSI    BstYI  BstZ17I     BtgI    BtgZI     BtsI   DraIII
     EarI  Eco57MI EcoO109I    Hin4I   HincII     MmeI     MslI     NcoI
     NspI    PflMI     PsrI     PstI     SfcI     StyI    TaqII     TatI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI   Bsu36I
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI