Restriction map for
pF1KB7163
(Linear) (Six-base) MAPSORT of: KIBB7163.seq Check: 8865 from: 1 to: 1164
KIBB7163.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 0 871 1164
Size: 871 293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 516 595 700 738 896 1045 1164
Size: 75 441 79 105 38 158 149 119
Fragments arranged by size:
441 158 149 119 105 79 75 38
AgeI A'CCGG_T
Cuts at: 0 613 1164
Size: 613 551
AleI CACnn'nnGTG
Cuts at: 0 616 1164
Size: 616 548
ApoI r'AATT_y
Cuts at: 0 313 778 1164
Size: 313 465 386
Fragments arranged by size:
465 386 313
BanI G'GyrC_C
Cuts at: 0 1104 1164
Size: 1104 60
BanII G_rGCy'C
Cuts at: 0 32 146 203 1164
Size: 32 114 57 961
Fragments arranged by size:
961 114 57 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1164
Size: 688 476
Bme1580I G_kGCm'C
Cuts at: 0 107 1164
Size: 107 1057
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 508 856 909 1164
Size: 101 407 348 53 255
Fragments arranged by size:
407 348 255 101 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 361 1164
Size: 361 803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 673 691 1164
Size: 130 543 18 473
Fragments arranged by size:
543 473 130 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 1164
Size: 91 1073
BsaWI w'CCGG_w
Cuts at: 0 613 1164
Size: 613 551
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 1164
Size: 545 30 589
Fragments arranged by size:
589 545 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 950 1164
Size: 542 408 214
Fragments arranged by size:
542 408 214
BsiEI CG_ry'CG
Cuts at: 0 992 1164
Size: 992 172
BsiHKAI G_wGCw'C
Cuts at: 0 32 146 1164
Size: 32 114 1018
Fragments arranged by size:
1018 114 32
Bsp1286I G_dGCh'C
Cuts at: 0 32 107 146 203 1164
Size: 32 75 39 57 961
Fragments arranged by size:
961 75 57 39 32
BsrBI CCG'CTC
Cuts at: 0 556 1164
Size: 556 608
BsrFI r'CCGG_y
Cuts at: 0 613 1164
Size: 613 551
BsrGI T'GTAC_A
Cuts at: 0 836 1164
Size: 836 328
BssSI C'ACGA_G
Cuts at: 0 41 1164
Size: 41 1123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 651 1087 1164
Size: 618 33 436 77
Fragments arranged by size:
618 436 77 33
BstYI r'GATC_y
Cuts at: 0 14 879 1164
Size: 14 865 285
Fragments arranged by size:
865 285 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1164
Size: 384 780
EaeI y'GGCC_r
Cuts at: 0 488 923 989 1164
Size: 488 435 66 175
Fragments arranged by size:
488 435 175 66
EagI C'GGCC_G
Cuts at: 0 989 1164
Size: 989 175
EarI CTCTTCn'nnn_
Cuts at: 0 76 94 1164
Size: 76 18 1070
Fragments arranged by size:
1070 76 18
EcoICRI GAG'CTC
Cuts at: 0 30 144 1164
Size: 30 114 1020
Fragments arranged by size:
1020 114 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 130 516 595 673 691 700 738
Size: 75 55 386 79 78 18 9 38
Cuts at: 738 896 1045 1164
Size: 158 149 119
Fragments arranged by size:
386 158 149 119 79 78 75 55
38 18 9
EcoO109I rG'GnC_Cy
Cuts at: 0 135 1164
Size: 135 1029
EcoRI G'AATT_C
Cuts at: 0 778 1164
Size: 778 386
MscI TGG'CCA
Cuts at: 0 490 1164
Size: 490 674
MslI CAynn'nnrTG
Cuts at: 0 616 1164
Size: 616 548
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1091 1164
Size: 945 146 73
Fragments arranged by size:
945 146 73
PfoI T'CCnGG_A
Cuts at: 0 146 650 1164
Size: 146 504 514
Fragments arranged by size:
514 504 146
PpuMI rG'GwC_Cy
Cuts at: 0 135 1164
Size: 135 1029
SacI G_AGCT'C
Cuts at: 0 32 146 1164
Size: 32 114 1018
Fragments arranged by size:
1018 114 32
SexAI A'CCwGG_T
Cuts at: 0 802 1148 1164
Size: 802 346 16
Fragments arranged by size:
802 346 16
SgrAI Cr'CCGG_yG
Cuts at: 0 613 1164
Size: 613 551
SmlI C'TyrA_G
Cuts at: 0 10 1164
Size: 10 1154
StuI AGG'CCT
Cuts at: 0 668 742 1164
Size: 668 74 422
Fragments arranged by size:
668 422 74
TatI w'GTAC_w
Cuts at: 0 836 1164
Size: 836 328
Tth111I GACn'n_nGTC
Cuts at: 0 1053 1164
Size: 1053 111
Enzymes that do cut:
AccI AcuI AgeI AleI ApoI BanI BanII BbsI
Bme1580I BmrI BplI BpmI BsaI BsaWI BsaXI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssSI BstXI
BstYI BtgZI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI MscI MslI PflMI PfoI PpuMI SacI SexAI
SgrAI SmlI StuI TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TstI XbaI XcmI XhoI XmaI XmnI ZraI