Restriction map for
pF1KB7161
(Linear) (Six-base) MAPSORT of: KIBB7161.seq Check: 1350 from: 1 to: 1119
KIBB7161.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AclI AA'CG_TT
Cuts at: 0 32 1119
Size: 32 1087
AhdI GACnn_n'nnGTC
Cuts at: 0 399 1119
Size: 399 720
AleI CACnn'nnGTG
Cuts at: 0 614 1119
Size: 614 505
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1119
Size: 387 732
ApaI G_GGCC'C
Cuts at: 0 604 1119
Size: 604 515
ApoI r'AATT_y
Cuts at: 0 892 1119
Size: 892 227
AvaI C'yCGr_G
Cuts at: 0 673 1119
Size: 673 446
AvrII C'CTAG_G
Cuts at: 0 808 1119
Size: 808 311
BanI G'GyrC_C
Cuts at: 0 210 373 864 1119
Size: 210 163 491 255
Fragments arranged by size:
491 255 210 163
BanII G_rGCy'C
Cuts at: 0 304 331 555 604 818 915 1119
Size: 304 27 224 49 214 97 204
Fragments arranged by size:
304 224 214 204 97 49 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 1119
Size: 74 1045
BciVI GTATCCnnnnn_n'
Cuts at: 0 360 1119
Size: 360 759
Bme1580I G_kGCm'C
Cuts at: 0 215 604 869 1119
Size: 215 389 265 250
Fragments arranged by size:
389 265 250 215
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 245 1119
Size: 81 164 874
Fragments arranged by size:
874 164 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 231 1119
Size: 132 99 888
Fragments arranged by size:
888 132 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1119
Size: 871 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1119
Size: 529 590
BsaBI GATnn'nnATC
Cuts at: 0 511 1119
Size: 511 608
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 1119
Size: 28 30 1061
Fragments arranged by size:
1061 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 121 214 217 220 303 355 448
Size: 108 13 93 3 3 83 52 93
Cuts at: 448 490 751 808 869 1119
Size: 42 261 57 61 250
Fragments arranged by size:
261 250 108 93 93 83 61 57
52 42 13 3 3
BseYI C'CCAG_C
Cuts at: 0 778 1119
Size: 778 341
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1119
Size: 856 263
BsiHKAI G_wGCw'C
Cuts at: 0 304 331 791 818 1119
Size: 304 27 460 27 301
Fragments arranged by size:
460 304 301 27 27
BsmI GAATG_Cn'
Cuts at: 0 245 765 1119
Size: 245 520 354
Fragments arranged by size:
520 354 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1110 1119
Size: 1110 9
Bsp1286I G_dGCh'C
Cuts at: 0 215 304 331 555 604 791 818 869
Size: 215 89 27 224 49 187 27 51
Cuts at: 869 915 1119
Size: 46 204
Fragments arranged by size:
224 215 204 187 89 51 49 46
27 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1057 1119
Size: 1057 62
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1068 1119
Size: 1068 51
Bsu36I CC'TnA_GG
Cuts at: 0 197 1119
Size: 197 922
EaeI y'GGCC_r
Cuts at: 0 434 1070 1119
Size: 434 636 49
Fragments arranged by size:
636 434 49
EarI CTCTTCn'nnn_
Cuts at: 0 98 132 162 180 1016 1119
Size: 98 34 30 18 836 103
Fragments arranged by size:
836 103 98 34 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 111 1119
Size: 111 1008
EcoICRI GAG'CTC
Cuts at: 0 302 329 816 1119
Size: 302 27 487 303
Fragments arranged by size:
487 303 302 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1119
Size: 871 248
EcoO109I rG'GnC_Cy
Cuts at: 0 268 280 384 601 1119
Size: 268 12 104 217 518
Fragments arranged by size:
518 268 217 104 12
EcoRI G'AATT_C
Cuts at: 0 892 1119
Size: 892 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 216 248 385 417 1119
Size: 28 32 156 32 137 32 702
Fragments arranged by size:
702 156 137 32 32 32 28
HincII GTy'rAC
Cuts at: 0 36 1119
Size: 36 1083
MfeI C'AATT_G
Cuts at: 0 1040 1119
Size: 1040 79
MscI TGG'CCA
Cuts at: 0 1072 1119
Size: 1072 47
MslI CAynn'nnrTG
Cuts at: 0 614 1066 1119
Size: 614 452 53
Fragments arranged by size:
614 452 53
NspI r_CATG'y
Cuts at: 0 672 1119
Size: 672 447
PpuMI rG'GwC_Cy
Cuts at: 0 268 280 1119
Size: 268 12 839
Fragments arranged by size:
839 268 12
PspOMI G'GGCC_C
Cuts at: 0 600 1119
Size: 600 519
SacI G_AGCT'C
Cuts at: 0 304 331 818 1119
Size: 304 27 487 301
Fragments arranged by size:
487 304 301 27
ScaI AGT'ACT
Cuts at: 0 941 1119
Size: 941 178
SmlI C'TyrA_G
Cuts at: 0 544 1119
Size: 544 575
SphI G_CATG'C
Cuts at: 0 672 1119
Size: 672 447
StuI AGG'CCT
Cuts at: 0 638 1119
Size: 638 481
StyI C'CwwG_G
Cuts at: 0 808 1119
Size: 808 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 822 1119
Size: 822 297
TatI w'GTAC_w
Cuts at: 0 137 518 939 1119
Size: 137 381 421 180
Fragments arranged by size:
421 381 180 137
Enzymes that do cut:
AclI AhdI AleI AlwNI ApaI ApoI AvaI AvrII
BanI BanII BbsI BciVI Bme1580I BmrI BplI BpmI
BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BstAPI BstXI Bsu36I EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MfeI MscI
MslI NspI PpuMI PspOMI SacI ScaI SmlI SphI
StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaHI BsaWI
BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI