Restriction map for
pF1KB7154
(Linear) (Six-base) MAPSORT of: KIBB7154.seq Check: 9749 from: 1 to: 1059
KIBB7154.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 338 1059
Size: 338 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1059
Size: 612 447
AflIII A'CryG_T
Cuts at: 0 575 720 1059
Size: 575 145 339
Fragments arranged by size:
575 339 145
ApaI G_GGCC'C
Cuts at: 0 399 972 1059
Size: 399 573 87
Fragments arranged by size:
573 399 87
ApoI r'AATT_y
Cuts at: 0 477 680 1059
Size: 477 203 379
Fragments arranged by size:
477 379 203
BanII G_rGCy'C
Cuts at: 0 399 972 1059
Size: 399 573 87
Fragments arranged by size:
573 399 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 717 1059
Size: 105 612 342
Fragments arranged by size:
612 342 105
BclI T'GATC_A
Cuts at: 0 344 1059
Size: 344 715
Bme1580I G_kGCm'C
Cuts at: 0 399 972 1059
Size: 399 573 87
Fragments arranged by size:
573 399 87
Bpu10I CC'TnA_GC
Cuts at: 0 50 1059
Size: 50 1009
BsaI GGTCTCn'nnnn_
Cuts at: 0 252 1059
Size: 252 807
BsaAI yAC'GTr
Cuts at: 0 721 1059
Size: 721 338
BsaBI GATnn'nnATC
Cuts at: 0 316 349 1059
Size: 316 33 710
Fragments arranged by size:
710 316 33
BsaWI w'CCGG_w
Cuts at: 0 158 1059
Size: 158 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1059
Size: 1030 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1051 1059
Size: 52 999 8
Fragments arranged by size:
999 52 8
BsiHKAI G_wGCw'C
Cuts at: 0 47 1059
Size: 47 1012
BsmI GAATG_Cn'
Cuts at: 0 541 782 1059
Size: 541 241 277
Fragments arranged by size:
541 277 241
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1033 1059
Size: 1033 26
Bsp1286I G_dGCh'C
Cuts at: 0 47 399 972 1059
Size: 47 352 573 87
Fragments arranged by size:
573 352 87 47
BspEI T'CCGG_A
Cuts at: 0 158 1059
Size: 158 901
BsrDI GCAATG_nn'
Cuts at: 0 85 135 353 1059
Size: 85 50 218 706
Fragments arranged by size:
706 218 85 50
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 960 1059
Size: 420 540 99
Fragments arranged by size:
540 420 99
BstYI r'GATC_y
Cuts at: 0 77 1059
Size: 77 982
Bsu36I CC'TnA_GG
Cuts at: 0 1034 1059
Size: 1034 25
BtgI C'CryG_G
Cuts at: 0 96 587 1059
Size: 96 491 472
Fragments arranged by size:
491 472 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 391 654 1059
Size: 391 263 405
Fragments arranged by size:
405 391 263
BtsI GCAGTG_nn'
Cuts at: 0 554 1059
Size: 554 505
EaeI y'GGCC_r
Cuts at: 0 60 422 590 1059
Size: 60 362 168 469
Fragments arranged by size:
469 362 168 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1059
Size: 612 447
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 1059
Size: 976 83
EcoO109I rG'GnC_Cy
Cuts at: 0 120 968 969 981 1059
Size: 120 848 1 12 78
Fragments arranged by size:
848 120 78 12 1
HincII GTy'rAC
Cuts at: 0 177 1059
Size: 177 882
MscI TGG'CCA
Cuts at: 0 62 424 592 1059
Size: 62 362 168 467
Fragments arranged by size:
467 362 168 62
MslI CAynn'nnrTG
Cuts at: 0 288 586 787 1059
Size: 288 298 201 272
Fragments arranged by size:
298 288 272 201
MspA1I CmG'CkG
Cuts at: 0 368 884 1059
Size: 368 516 175
Fragments arranged by size:
516 368 175
NcoI C'CATG_G
Cuts at: 0 587 1059
Size: 587 472
PpuMI rG'GwC_Cy
Cuts at: 0 120 981 1059
Size: 120 861 78
Fragments arranged by size:
861 120 78
PshAI GACnn'nnGTC
Cuts at: 0 979 1059
Size: 979 80
PspOMI G'GGCC_C
Cuts at: 0 395 968 1059
Size: 395 573 91
Fragments arranged by size:
573 395 91
PvuII CAG'CTG
Cuts at: 0 884 1059
Size: 884 175
ScaI AGT'ACT
Cuts at: 0 504 1059
Size: 504 555
SfcI C'TryA_G
Cuts at: 0 560 1059
Size: 560 499
SpeI A'CTAG_T
Cuts at: 0 955 1059
Size: 955 104
StyI C'CwwG_G
Cuts at: 0 587 593 866 1059
Size: 587 6 273 193
Fragments arranged by size:
587 273 193 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 1059
Size: 135 924
TatI w'GTAC_w
Cuts at: 0 502 1059
Size: 502 557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 619 1059
Size: 357 262 440
Fragments arranged by size:
440 357 262
XmnI GAAnn'nnTTC
Cuts at: 0 577 1059
Size: 577 482
Enzymes that do cut:
AccI AcuI AflIII ApaI ApoI BanII BbsI BclI
Bme1580I Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI Eco57MI EcoNI EcoO109I
HincII MscI MslI MspA1I NcoI PpuMI PshAI PspOMI
PvuII ScaI SfcI SpeI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI BpuEI BsaHI BseYI BsgI BsiEI BsiWI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI