Restriction map for
pF1KB7150
(Linear) (Six-base) MAPSORT of: KIBB7150.seq Check: 1400 from: 1 to: 1041
KIBB7150.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 919 1041
Size: 919 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1041
Size: 523 518
ApaI G_GGCC'C
Cuts at: 0 829 1041
Size: 829 212
ApaLI G'TGCA_C
Cuts at: 0 998 1041
Size: 998 43
ApoI r'AATT_y
Cuts at: 0 904 1041
Size: 904 137
AvaI C'yCGr_G
Cuts at: 0 5 53 1041
Size: 5 48 988
Fragments arranged by size:
988 48 5
BanII G_rGCy'C
Cuts at: 0 392 731 829 837 1041
Size: 392 339 98 8 204
Fragments arranged by size:
392 339 204 98 8
BbvCI CC'TCA_GC
Cuts at: 0 656 1041
Size: 656 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 633 667 1041
Size: 633 34 374
Fragments arranged by size:
633 374 34
BglII A'GATC_T
Cuts at: 0 294 552 1041
Size: 294 258 489
Fragments arranged by size:
489 294 258
Bme1580I G_kGCm'C
Cuts at: 0 596 791 829 1002 1041
Size: 596 195 38 173 39
Fragments arranged by size:
596 195 173 39 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 893 1041
Size: 893 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 793 1041
Size: 499 294 248
Fragments arranged by size:
499 294 248
Bpu10I CC'TnA_GC
Cuts at: 0 457 656 1041
Size: 457 199 385
Fragments arranged by size:
457 385 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1041
Size: 886 155
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 115 673 1041
Size: 71 44 558 368
Fragments arranged by size:
558 368 71 44
BsiHKAI G_wGCw'C
Cuts at: 0 476 713 1002 1041
Size: 476 237 289 39
Fragments arranged by size:
476 289 237 39
Bsp1286I G_dGCh'C
Cuts at: 0 392 476 596 713 731 791 829 837
Size: 392 84 120 117 18 60 38 8
Cuts at: 837 1002 1041
Size: 165 39
Fragments arranged by size:
392 165 120 117 84 60 39 38
18 8
BsrFI r'CCGG_y
Cuts at: 0 994 1041
Size: 994 47
BsrGI T'GTAC_A
Cuts at: 0 368 1041
Size: 368 673
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 1041
Size: 363 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 1041
Size: 753 288
BstYI r'GATC_y
Cuts at: 0 294 552 1041
Size: 294 258 489
Fragments arranged by size:
489 294 258
BstZ17I GTA'TAC
Cuts at: 0 920 1041
Size: 920 121
BtgI C'CryG_G
Cuts at: 0 183 1041
Size: 183 858
EaeI y'GGCC_r
Cuts at: 0 875 1041
Size: 875 166
EarI CTCTTCn'nnn_
Cuts at: 0 10 92 938 1041
Size: 10 82 846 103
Fragments arranged by size:
846 103 82 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 523 793 1041
Size: 499 24 270 248
Fragments arranged by size:
499 270 248 24
EcoO109I rG'GnC_Cy
Cuts at: 0 325 825 1041
Size: 325 500 216
Fragments arranged by size:
500 325 216
EcoRI G'AATT_C
Cuts at: 0 904 1041
Size: 904 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 94 98 126 154 186 477 509
Size: 66 28 4 28 28 32 291 32
Cuts at: 509 1041
Size: 532
Fragments arranged by size:
532 291 66 32 32 28 28 28
4
HincII GTy'rAC
Cuts at: 0 258 543 1041
Size: 258 285 498
Fragments arranged by size:
498 285 258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1041
Size: 197 844
MscI TGG'CCA
Cuts at: 0 877 1041
Size: 877 164
MslI CAynn'nnrTG
Cuts at: 0 530 1041
Size: 530 511
MspA1I CmG'CkG
Cuts at: 0 226 1041
Size: 226 815
NcoI C'CATG_G
Cuts at: 0 183 1041
Size: 183 858
PspOMI G'GGCC_C
Cuts at: 0 825 1041
Size: 825 216
PspXI vC'TCGA_Gb
Cuts at: 0 5 1041
Size: 5 1036
PstI C_TGCA'G
Cuts at: 0 1040 1041
Size: 1040 1
PvuII CAG'CTG
Cuts at: 0 226 1041
Size: 226 815
SfcI C'TryA_G
Cuts at: 0 221 329 1036 1041
Size: 221 108 707 5
Fragments arranged by size:
707 221 108 5
SmlI C'TyrA_G
Cuts at: 0 5 865 1041
Size: 5 860 176
Fragments arranged by size:
860 176 5
StyI C'CwwG_G
Cuts at: 0 183 878 971 1007 1041
Size: 183 695 93 36 34
Fragments arranged by size:
695 183 93 36 34
TatI w'GTAC_w
Cuts at: 0 368 1041
Size: 368 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 228 260 1041
Size: 228 32 781
Fragments arranged by size:
781 228 32
XbaI T'CTAG_A
Cuts at: 0 297 1041
Size: 297 744
XhoI C'TCGA_G
Cuts at: 0 5 1041
Size: 5 1036
Enzymes that do cut:
AccI AcuI ApaI ApaLI ApoI AvaI BanII BbvCI
BcgI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BseRI
BsiHKAI Bsp1286I BsrFI BsrGI BstAPI BstXI BstYI BstZ17I
BtgI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII
MmeI MscI MslI MspA1I NcoI PspOMI PspXI PstI
PvuII SfcI SmlI StyI TatI TstI XbaI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII
BssSI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XcmI XmaI XmnI ZraI