Restriction map for
pF1KB7146
(Linear) (Six-base) MAPSORT of: KIBB7146.seq Check: 3371 from: 1 to: 1038
KIBB7146.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 56 1038
Size: 56 982
AleI CACnn'nnGTG
Cuts at: 0 77 1038
Size: 77 961
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1038
Size: 363 675
ApoI r'AATT_y
Cuts at: 0 673 814 1038
Size: 673 141 224
Fragments arranged by size:
673 224 141
AvaI C'yCGr_G
Cuts at: 0 5 53 1038
Size: 5 48 985
Fragments arranged by size:
985 48 5
BanII G_rGCy'C
Cuts at: 0 731 837 1038
Size: 731 106 201
Fragments arranged by size:
731 201 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 346 1038
Size: 346 692
BciVI GTATCCnnnnn_n'
Cuts at: 0 123 1038
Size: 123 915
BglII A'GATC_T
Cuts at: 0 665 1038
Size: 665 373
Bme1580I G_kGCm'C
Cuts at: 0 791 1038
Size: 791 247
BmrI ACTGGGnnnn_n'
Cuts at: 0 893 1038
Size: 893 145
BmtI G_CTAG'C
Cuts at: 0 1022 1038
Size: 1022 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 886 1038
Size: 793 93 152
Fragments arranged by size:
793 152 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1038
Size: 169 869
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 1038
Size: 610 428
BsaAI yAC'GTr
Cuts at: 0 34 175 1038
Size: 34 141 863
Fragments arranged by size:
863 141 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 472 502 1038
Size: 472 30 536
Fragments arranged by size:
536 472 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 1038
Size: 673 365
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 376 788 1038
Size: 331 45 412 250
Fragments arranged by size:
412 331 250 45
BsiHKAI G_wGCw'C
Cuts at: 0 84 183 204 1038
Size: 84 99 21 834
Fragments arranged by size:
834 99 84 21
Bsp1286I G_dGCh'C
Cuts at: 0 84 183 204 731 791 837 1038
Size: 84 99 21 527 60 46 201
Fragments arranged by size:
527 201 99 84 60 46 21
BspHI T'CATG_A
Cuts at: 0 635 1038
Size: 635 403
BsrGI T'GTAC_A
Cuts at: 0 374 1038
Size: 374 664
BssSI C'ACGA_G
Cuts at: 0 578 1038
Size: 578 460
BstYI r'GATC_y
Cuts at: 0 452 552 665 1038
Size: 452 100 113 373
Fragments arranged by size:
452 373 113 100
BtgI C'CryG_G
Cuts at: 0 989 1038
Size: 989 49
BtsI GCAGTG_nn'
Cuts at: 0 89 1038
Size: 89 949
DraI TTT'AAA
Cuts at: 0 503 1038
Size: 503 535
DraIII CAC_nnn'GTG
Cuts at: 0 178 1038
Size: 178 860
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 1038
Size: 532 506
EarI CTCTTCn'nnn_
Cuts at: 0 10 917 1032 1038
Size: 10 907 115 6
Fragments arranged by size:
907 115 10 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 886 1038
Size: 793 93 152
Fragments arranged by size:
793 152 93
EcoO109I rG'GnC_Cy
Cuts at: 0 861 1038
Size: 861 177
EcoRI G'AATT_C
Cuts at: 0 673 814 1038
Size: 673 141 224
Fragments arranged by size:
673 224 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 459 729 1038
Size: 300 159 270 309
Fragments arranged by size:
309 300 270 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 54 74 86 561 593 1038
Size: 42 12 20 12 475 32 445
Fragments arranged by size:
475 445 42 32 20 12 12
HindIII A'AGCT_T
Cuts at: 0 282 464 760 1038
Size: 282 182 296 278
Fragments arranged by size:
296 282 278 182
MslI CAynn'nnrTG
Cuts at: 0 32 77 481 1038
Size: 32 45 404 557
Fragments arranged by size:
557 404 45 32
NcoI C'CATG_G
Cuts at: 0 989 1038
Size: 989 49
NheI G'CTAG_C
Cuts at: 0 1018 1038
Size: 1018 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 1038
Size: 753 285
PfoI T'CCnGG_A
Cuts at: 0 863 1038
Size: 863 175
PmlI CAC'GTG
Cuts at: 0 34 1038
Size: 34 1004
PpuMI rG'GwC_Cy
Cuts at: 0 861 1038
Size: 861 177
PstI C_TGCA'G
Cuts at: 0 963 1038
Size: 963 75
SapI GCTCTTCn'nnn_
Cuts at: 0 1032 1038
Size: 1032 6
SfcI C'TryA_G
Cuts at: 0 221 959 1038
Size: 221 738 79
Fragments arranged by size:
738 221 79
SmaI CCC'GGG
Cuts at: 0 55 1038
Size: 55 983
SmlI C'TyrA_G
Cuts at: 0 184 1038
Size: 184 854
StyI C'CwwG_G
Cuts at: 0 521 878 989 1007 1013 1038
Size: 521 357 111 18 6 25
Fragments arranged by size:
521 357 111 25 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 1038
Size: 760 278
TatI w'GTAC_w
Cuts at: 0 374 1038
Size: 374 664
XmaI C'CCGG_G
Cuts at: 0 53 1038
Size: 53 985
XmnI GAAnn'nnTTC
Cuts at: 0 442 712 1002 1038
Size: 442 270 290 36
Fragments arranged by size:
442 290 270 36
Enzymes that do cut:
AhdI AleI AlwNI ApoI AvaI BanII BbsI BciVI
BglII Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrGI BssSI
BstYI BtgI BtsI DraI DraIII DrdI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HindIII MslI NcoI NheI
PflMI PfoI PmlI PpuMI PstI SapI SfcI SmaI
SmlI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BlpI BmgBI BplI Bpu10I BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI EaeI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI ZraI