(Linear) (Six-base) MAPSORT of: KIBB7142.seq  Check: 8487  from: 1  to: 1011



KIBB7142.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315    1011 
   Size:        315     696
 
AflII C'TTAA_G

Cuts at:      0     703    1011 
   Size:        703     308
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     253    1011 
   Size:        253     758
 
BclI T'GATC_A

Cuts at:      0     401     512    1011 
   Size:        401     111     499

  Fragments arranged by size: 

                 499     401     111
 
BglII A'GATC_T

Cuts at:      0     234    1011 
   Size:        234     777
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     798     994    1011 
   Size:         76     722     196      17

  Fragments arranged by size: 

                 722     196      76      17
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     180    1011 
   Size:        180     831
 
BsaAI yAC'GTr

Cuts at:      0     753    1011 
   Size:        753     258
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     467    1011 
   Size:        213     254     544

  Fragments arranged by size: 

                 544     254     213
 
BsrDI GCAATG_nn'

Cuts at:      0     156    1011 
   Size:        156     855
 
BssSI C'ACGA_G

Cuts at:      0     133    1011 
   Size:        133     878
 
BstEII G'GTnAC_C

Cuts at:      0     889    1011 
   Size:        889     122
 
BstYI r'GATC_y

Cuts at:      0     234     296     836    1011 
   Size:        234      62     540     175

  Fragments arranged by size: 

                 540     234     175      62
 
BtgI C'CryG_G

Cuts at:      0     678    1011 
   Size:        678     333
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     370    1011 
   Size:        190     180     641

  Fragments arranged by size: 

                 641     190     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315    1011 
   Size:        315     696
 
EcoRV GAT'ATC

Cuts at:      0     164    1011 
   Size:        164     847
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     783     815    1011 
   Size:        783      32     196

  Fragments arranged by size: 

                 783     196      32
 
HindIII A'AGCT_T

Cuts at:      0     845    1011 
   Size:        845     166
 
MslI CAynn'nnrTG

Cuts at:      0     211     275     499    1011 
   Size:        211      64     224     512

  Fragments arranged by size: 

                 512     224     211      64
 
MspA1I CmG'CkG

Cuts at:      0      55     698    1011 
   Size:         55     643     313

  Fragments arranged by size: 

                 643     313      55
 
NcoI C'CATG_G

Cuts at:      0     678    1011 
   Size:        678     333
 
PvuII CAG'CTG

Cuts at:      0      55     698    1011 
   Size:         55     643     313

  Fragments arranged by size: 

                 643     313      55
 
SapI GCTCTTCn'nnn_

Cuts at:      0     190    1011 
   Size:        190     821
 
SmlI C'TyrA_G

Cuts at:      0      91     703     777     973    1011 
   Size:         91     612      74     196      38

  Fragments arranged by size: 

                 612     196      91      74      38
 
SnaBI TAC'GTA

Cuts at:      0     753    1011 
   Size:        753     258
 
StyI C'CwwG_G

Cuts at:      0     190     629     678    1011 
   Size:        190     439      49     333

  Fragments arranged by size: 

                 439     333     190      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     425    1011 
   Size:        425     586
 
XbaI T'CTAG_A

Cuts at:      0     859    1011 
   Size:        859     152
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     BclI    BglII    BpuEI     BsaI    BsaAI
     BsgI    BsrDI    BssSI   BstEII    BstYI     BtgI     EarI  Eco57MI
    EcoRV    Hin4I  HindIII     MslI   MspA1I     NcoI    PvuII     SapI
     SmlI    SnaBI     StyI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI