Restriction map for
pF1KB7139
(Linear) (Six-base) MAPSORT of: KIBB7139.seq Check: 2022 from: 1 to: 999
KIBB7139.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 698 999
Size: 698 301
AccI GT'mk_AC
Cuts at: 0 272 999
Size: 272 727
AhdI GACnn_n'nnGTC
Cuts at: 0 144 999
Size: 144 855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 999
Size: 505 494
ApaI G_GGCC'C
Cuts at: 0 818 999
Size: 818 181
ApaLI G'TGCA_C
Cuts at: 0 685 999
Size: 685 314
BanI G'GyrC_C
Cuts at: 0 457 521 757 999
Size: 457 64 236 242
Fragments arranged by size:
457 242 236 64
BanII G_rGCy'C
Cuts at: 0 548 818 851 999
Size: 548 270 33 148
Fragments arranged by size:
548 270 148 33
BbeI G_GCGC'C
Cuts at: 0 525 999
Size: 525 474
BbsI GAAGACnn'nnnn_
Cuts at: 0 622 780 896 999
Size: 622 158 116 103
Fragments arranged by size:
622 158 116 103
BbvCI CC'TCA_GC
Cuts at: 0 706 999
Size: 706 293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 481 554 588 999
Size: 447 34 73 34 411
Fragments arranged by size:
447 411 73 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 119 530 999
Size: 119 411 469
Fragments arranged by size:
469 411 119
BglII A'GATC_T
Cuts at: 0 68 407 999
Size: 68 339 592
Fragments arranged by size:
592 339 68
Bme1580I G_kGCm'C
Cuts at: 0 345 460 689 726 760 818 999
Size: 345 115 229 37 34 58 181
Fragments arranged by size:
345 229 181 115 58 37 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 240 879 999
Size: 18 222 639 120
Fragments arranged by size:
639 222 120 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 697 999
Size: 40 657 302
Fragments arranged by size:
657 302 40
Bpu10I CC'TnA_GC
Cuts at: 0 706 999
Size: 706 293
BsaHI Gr'CG_yC
Cuts at: 0 522 901 999
Size: 522 379 98
Fragments arranged by size:
522 379 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 999
Size: 535 30 434
Fragments arranged by size:
535 434 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 753 904 999
Size: 753 151 95
Fragments arranged by size:
753 151 95
BseYI C'CCAG_C
Cuts at: 0 170 967 999
Size: 170 797 32
Fragments arranged by size:
797 170 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 999
Size: 165 834
BsiEI CG_ry'CG
Cuts at: 0 56 386 999
Size: 56 330 613
Fragments arranged by size:
613 330 56
BsiHKAI G_wGCw'C
Cuts at: 0 50 86 450 479 570 689 999
Size: 50 36 364 29 91 119 310
Fragments arranged by size:
364 310 119 91 50 36 29
Bsp1286I G_dGCh'C
Cuts at: 0 50 86 345 450 460 479 548 570
Size: 50 36 259 105 10 19 69 22
Cuts at: 570 689 726 760 818 851 999
Size: 119 37 34 58 33 148
Fragments arranged by size:
259 148 119 105 69 58 50 37
36 34 33 22 19 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 698 999
Size: 680 18 301
Fragments arranged by size:
680 301 18
BsrBI CCG'CTC
Cuts at: 0 312 372 999
Size: 312 60 627
Fragments arranged by size:
627 312 60
BsrFI r'CCGG_y
Cuts at: 0 118 348 999
Size: 118 230 651
Fragments arranged by size:
651 230 118
BssSI C'ACGA_G
Cuts at: 0 583 999
Size: 583 416
BstYI r'GATC_y
Cuts at: 0 68 407 999
Size: 68 339 592
Fragments arranged by size:
592 339 68
BtgI C'CryG_G
Cuts at: 0 56 441 539 608 803 999
Size: 56 385 98 69 195 196
Fragments arranged by size:
385 196 195 98 69 56
BtsI GCAGTG_nn'
Cuts at: 0 343 999
Size: 343 656
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 999
Size: 595 404
EaeI y'GGCC_r
Cuts at: 0 116 350 383 401 513 945 999
Size: 116 234 33 18 112 432 54
Fragments arranged by size:
432 234 116 112 54 33 18
EagI C'GGCC_G
Cuts at: 0 383 999
Size: 383 616
EarI CTCTTCn'nnn_
Cuts at: 0 91 253 302 925 999
Size: 91 162 49 623 74
Fragments arranged by size:
623 162 91 74 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 697 999
Size: 40 657 302
Fragments arranged by size:
657 302 40
EcoO109I rG'GnC_Cy
Cuts at: 0 357 814 815 911 999
Size: 357 457 1 96 88
Fragments arranged by size:
457 357 96 88 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 639 999
Size: 639 360
FspI TGC'GCA
Cuts at: 0 436 999
Size: 436 563
HaeII r_GCGC'y
Cuts at: 0 379 525 999
Size: 379 146 474
Fragments arranged by size:
474 379 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 999
Size: 130 32 837
Fragments arranged by size:
837 130 32
KasI G'GCGC_C
Cuts at: 0 521 999
Size: 521 478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 999
Size: 627 372
MscI TGG'CCA
Cuts at: 0 403 515 947 999
Size: 403 112 432 52
Fragments arranged by size:
432 403 112 52
MslI CAynn'nnrTG
Cuts at: 0 634 640 999
Size: 634 6 359
Fragments arranged by size:
634 359 6
MspA1I CmG'CkG
Cuts at: 0 443 999
Size: 443 556
NaeI GCC'GGC
Cuts at: 0 120 350 999
Size: 120 230 649
Fragments arranged by size:
649 230 120
NarI GG'CG_CC
Cuts at: 0 522 999
Size: 522 477
NcoI C'CATG_G
Cuts at: 0 539 999
Size: 539 460
NgoMIV G'CCGG_C
Cuts at: 0 118 348 999
Size: 118 230 651
Fragments arranged by size:
651 230 118
PasI CC'CwG_GG
Cuts at: 0 753 999
Size: 753 246
PfoI T'CCnGG_A
Cuts at: 0 17 41 923 999
Size: 17 24 882 76
Fragments arranged by size:
882 76 24 17
PpuMI rG'GwC_Cy
Cuts at: 0 357 999
Size: 357 642
PspOMI G'GGCC_C
Cuts at: 0 814 999
Size: 814 185
PstI C_TGCA'G
Cuts at: 0 242 999
Size: 242 757
SacII CC_GC'GG
Cuts at: 0 444 999
Size: 444 555
SapI GCTCTTCn'nnn_
Cuts at: 0 91 302 999
Size: 91 211 697
Fragments arranged by size:
697 211 91
SexAI A'CCwGG_T
Cuts at: 0 35 782 999
Size: 35 747 217
Fragments arranged by size:
747 217 35
SfcI C'TryA_G
Cuts at: 0 238 999
Size: 238 761
SfoI GGC'GCC
Cuts at: 0 523 999
Size: 523 476
StuI AGG'CCT
Cuts at: 0 205 999
Size: 205 794
StyI C'CwwG_G
Cuts at: 0 353 539 999
Size: 353 186 460
Fragments arranged by size:
460 353 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 276 999
Size: 222 54 723
Fragments arranged by size:
723 222 54
Tth111I GACn'n_nGTC
Cuts at: 0 37 784 999
Size: 37 747 215
Fragments arranged by size:
747 215 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 999
Size: 411 588
Enzymes that do cut:
AarI AccI AhdI AlfI ApaI ApaLI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstYI BtgI
BtsI DrdI EaeI EagI EarI Eco57MI EcoO109I FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PfoI PpuMI PspOMI
PstI SacII SapI SexAI SfcI SfoI StuI StyI
TaqII Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XhoI XmaI XmnI
ZraI