(Linear) (Six-base) MAPSORT of: KIBB7135.seq  Check: 4158  from: 1  to: 963



KIBB7135.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     170     963 
   Size:        170     793
 
AflIII A'CryG_T

Cuts at:      0     718     963 
   Size:        718     245
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     456     963 
   Size:        456     507
 
ApaLI G'TGCA_C

Cuts at:      0     247     963 
   Size:        247     716
 
ApoI r'AATT_y

Cuts at:      0      31     963 
   Size:         31     932
 
AseI AT'TA_AT

Cuts at:      0     130     521     963 
   Size:        130     391     442

  Fragments arranged by size: 

                 442     391     130
 
BamHI G'GATC_C

Cuts at:      0     680     963 
   Size:        680     283
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     603     705     963 
   Size:        603     102     258

  Fragments arranged by size: 

                 603     258     102
 
Bme1580I G_kGCm'C

Cuts at:      0     251     963 
   Size:        251     712
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     614     963 
   Size:        614     349
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     696     963 
   Size:        696     267
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     963 
   Size:        244     719
 
BseYI C'CCAG_C

Cuts at:      0     443     963 
   Size:        443     520
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     963 
   Size:        251     712
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     963 
   Size:        251     712
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     963 
   Size:        170     793
 
BsrDI GCAATG_nn'

Cuts at:      0     234     356     368     963 
   Size:        234     122      12     595

  Fragments arranged by size: 

                 595     234     122      12
 
BsrGI T'GTAC_A

Cuts at:      0     830     963 
   Size:        830     133
 
BstYI r'GATC_y

Cuts at:      0     680     963 
   Size:        680     283
 
BtsI GCAGTG_nn'

Cuts at:      0     592     963 
   Size:        592     371
 
EaeI y'GGCC_r

Cuts at:      0     734     963 
   Size:        734     229
 
EcoRI G'AATT_C

Cuts at:      0      31     963 
   Size:         31     932
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     293     963 
   Size:        293     670
 
HaeII r_GCGC'y

Cuts at:      0     764     963 
   Size:        764     199
 
HincII GTy'rAC

Cuts at:      0     485     963 
   Size:        485     478
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     601     963 
   Size:         77     524     362

  Fragments arranged by size: 

                 524     362      77
 
MscI TGG'CCA

Cuts at:      0     736     963 
   Size:        736     227
 
NdeI CA'TA_TG

Cuts at:      0     357     774     963 
   Size:        357     417     189

  Fragments arranged by size: 

                 417     357     189
 
NspI r_CATG'y

Cuts at:      0     722     963 
   Size:        722     241
 
PciI A'CATG_T

Cuts at:      0     718     963 
   Size:        718     245
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     963 
   Size:        243     720
 
SfcI C'TryA_G

Cuts at:      0     908     963 
   Size:        908      55
 
SmlI C'TyrA_G

Cuts at:      0     675     963 
   Size:        675     288
 
TatI w'GTAC_w

Cuts at:      0     109     830     963 
   Size:        109     721     133

  Fragments arranged by size: 

                 721     133     109
 
XmnI GAAnn'nnTTC

Cuts at:      0     573     963 
   Size:        573     390
 

 Enzymes that do cut:

     AarI   AflIII     AlfI    ApaLI     ApoI     AseI    BamHI     BbsI
 Bme1580I     BmrI    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrGI    BstYI     BtsI     EaeI    EcoRI     FalI    HaeII
   HincII     MmeI     MscI     NdeI     NspI     PciI    PflMI     SfcI
     SmlI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     AscI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI