Restriction map for
pF1KB7134
(Linear) (Six-base) MAPSORT of: KIBB7134.seq Check: 4800 from: 1 to: 960
KIBB7134.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AleI CACnn'nnGTG
Cuts at: 0 31 731 960
Size: 31 700 229
Fragments arranged by size:
700 229 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 616 648 960
Size: 616 32 312
Fragments arranged by size:
616 312 32
AvaI C'yCGr_G
Cuts at: 0 263 960
Size: 263 697
BamHI G'GATC_C
Cuts at: 0 451 960
Size: 451 509
BanI G'GyrC_C
Cuts at: 0 874 960
Size: 874 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 126 960
Size: 126 834
BciVI GTATCCnnnnn_n'
Cuts at: 0 55 960
Size: 55 905
BclI T'GATC_A
Cuts at: 0 482 960
Size: 482 478
BsaBI GATnn'nnATC
Cuts at: 0 430 960
Size: 430 530
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 960
Size: 145 815
BsiHKAI G_wGCw'C
Cuts at: 0 404 960
Size: 404 556
Bsp1286I G_dGCh'C
Cuts at: 0 404 960
Size: 404 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 960
Size: 845 115
BsrBI CCG'CTC
Cuts at: 0 696 960
Size: 696 264
BsrDI GCAATG_nn'
Cuts at: 0 116 960
Size: 116 844
BsrGI T'GTAC_A
Cuts at: 0 777 960
Size: 777 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 342 609 960
Size: 207 135 267 351
Fragments arranged by size:
351 267 207 135
BstYI r'GATC_y
Cuts at: 0 236 451 960
Size: 236 215 509
Fragments arranged by size:
509 236 215
BtgI C'CryG_G
Cuts at: 0 347 447 960
Size: 347 100 513
Fragments arranged by size:
513 347 100
BtsI GCAGTG_nn'
Cuts at: 0 382 404 469 960
Size: 382 22 65 491
Fragments arranged by size:
491 382 65 22
EaeI y'GGCC_r
Cuts at: 0 344 368 960
Size: 344 24 592
Fragments arranged by size:
592 344 24
MscI TGG'CCA
Cuts at: 0 346 370 960
Size: 346 24 590
Fragments arranged by size:
590 346 24
MslI CAynn'nnrTG
Cuts at: 0 31 509 542 619 731 733 960
Size: 31 478 33 77 112 2 227
Fragments arranged by size:
478 227 112 77 33 31 2
MspA1I CmG'CkG
Cuts at: 0 449 926 960
Size: 449 477 34
Fragments arranged by size:
477 449 34
NcoI C'CATG_G
Cuts at: 0 347 960
Size: 347 613
NspI r_CATG'y
Cuts at: 0 25 192 960
Size: 25 167 768
Fragments arranged by size:
768 167 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 763 960
Size: 763 197
PstI C_TGCA'G
Cuts at: 0 399 960
Size: 399 561
PvuII CAG'CTG
Cuts at: 0 926 960
Size: 926 34
SacII CC_GC'GG
Cuts at: 0 450 960
Size: 450 510
SfcI C'TryA_G
Cuts at: 0 395 960
Size: 395 565
SphI G_CATG'C
Cuts at: 0 192 960
Size: 192 768
StuI AGG'CCT
Cuts at: 0 283 706 960
Size: 283 423 254
Fragments arranged by size:
423 283 254
StyI C'CwwG_G
Cuts at: 0 347 701 960
Size: 347 354 259
Fragments arranged by size:
354 347 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 395 814 960
Size: 395 419 146
Fragments arranged by size:
419 395 146
TatI w'GTAC_w
Cuts at: 0 777 960
Size: 777 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 704 736 960
Size: 704 32 224
Fragments arranged by size:
704 224 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 73 102 339 960
Size: 73 29 237 621
Fragments arranged by size:
621 237 73 29
Enzymes that do cut:
AleI AloI AvaI BamHI BanI BbsI BciVI BclI
BsaBI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstXI BstYI BtgI BtsI EaeI MscI MslI MspA1I
NcoI NspI PflMI PstI PvuII SacII SfcI SphI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI
BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI