Restriction map for
pF1KB7132
(Linear) (Six-base) MAPSORT of: KIBB7132.seq Check: 259 from: 1 to: 954
KIBB7132.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 474 556 954
Size: 474 82 398
Fragments arranged by size:
474 398 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 444 954
Size: 31 413 510
Fragments arranged by size:
510 413 31
AfeI AGC'GCT
Cuts at: 0 25 954
Size: 25 929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 954
Size: 128 826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 954
Size: 304 32 618
Fragments arranged by size:
618 304 32
ApaI G_GGCC'C
Cuts at: 0 840 954
Size: 840 114
BanI G'GyrC_C
Cuts at: 0 63 199 789 954
Size: 63 136 590 165
Fragments arranged by size:
590 165 136 63
BanII G_rGCy'C
Cuts at: 0 840 954
Size: 840 114
BbeI G_GCGC'C
Cuts at: 0 793 954
Size: 793 161
BbvCI CC'TCA_GC
Cuts at: 0 35 98 954
Size: 35 63 856
Fragments arranged by size:
856 63 35
BclI T'GATC_A
Cuts at: 0 134 585 954
Size: 134 451 369
Fragments arranged by size:
451 369 134
Bme1580I G_kGCm'C
Cuts at: 0 840 954
Size: 840 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 954
Size: 219 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 796 889 954
Size: 331 465 93 65
Fragments arranged by size:
465 331 93 65
Bpu10I CC'TnA_GC
Cuts at: 0 35 98 733 954
Size: 35 63 635 221
Fragments arranged by size:
635 221 63 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 436 954
Size: 169 267 518
Fragments arranged by size:
518 267 169
BsaHI Gr'CG_yC
Cuts at: 0 790 954
Size: 790 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 954
Size: 304 30 620
Fragments arranged by size:
620 304 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 114 115 166 195 289 676 954
Size: 111 3 1 51 29 94 387 278
Fragments arranged by size:
387 278 111 94 51 29 3 1
BseYI C'CCAG_C
Cuts at: 0 300 576 954
Size: 300 276 378
Fragments arranged by size:
378 300 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 781 954
Size: 103 678 173
Fragments arranged by size:
678 173 103
BsiHKAI G_wGCw'C
Cuts at: 0 716 954
Size: 716 238
Bsp1286I G_dGCh'C
Cuts at: 0 716 840 954
Size: 716 124 114
Fragments arranged by size:
716 124 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 556 954
Size: 474 82 398
Fragments arranged by size:
474 398 82
BsrDI GCAATG_nn'
Cuts at: 0 273 954
Size: 273 681
BsrFI r'CCGG_y
Cuts at: 0 393 523 604 954
Size: 393 130 81 350
Fragments arranged by size:
393 350 130 81
BstEII G'GTnAC_C
Cuts at: 0 170 954
Size: 170 784
Bsu36I CC'TnA_GG
Cuts at: 0 194 954
Size: 194 760
BtsI GCAGTG_nn'
Cuts at: 0 804 954
Size: 804 150
DraIII CAC_nnn'GTG
Cuts at: 0 236 954
Size: 236 718
EaeI y'GGCC_r
Cuts at: 0 525 954
Size: 525 429
EarI CTCTTCn'nnn_
Cuts at: 0 10 213 266 920 954
Size: 10 203 53 654 34
Fragments arranged by size:
654 203 53 34 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 331 444 796 889 954
Size: 31 300 113 352 93 65
Fragments arranged by size:
352 300 113 93 65 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 151 954
Size: 151 803
EcoO109I rG'GnC_Cy
Cuts at: 0 836 837 954
Size: 836 1 117
Fragments arranged by size:
836 117 1
EcoRV GAT'ATC
Cuts at: 0 456 954
Size: 456 498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 954
Size: 462 492
HaeII r_GCGC'y
Cuts at: 0 27 793 954
Size: 27 766 161
Fragments arranged by size:
766 161 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 954
Size: 154 32 768
Fragments arranged by size:
768 154 32
KasI G'GCGC_C
Cuts at: 0 789 954
Size: 789 165
MslI CAynn'nnrTG
Cuts at: 0 484 502 954
Size: 484 18 452
Fragments arranged by size:
484 452 18
MspA1I CmG'CkG
Cuts at: 0 97 954
Size: 97 857
NaeI GCC'GGC
Cuts at: 0 525 954
Size: 525 429
NarI GG'CG_CC
Cuts at: 0 790 954
Size: 790 164
NgoMIV G'CCGG_C
Cuts at: 0 523 954
Size: 523 431
NspI r_CATG'y
Cuts at: 0 483 877 954
Size: 483 394 77
Fragments arranged by size:
483 394 77
PfoI T'CCnGG_A
Cuts at: 0 323 866 954
Size: 323 543 88
Fragments arranged by size:
543 323 88
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 954
Size: 304 32 618
Fragments arranged by size:
618 304 32
PspOMI G'GGCC_C
Cuts at: 0 836 954
Size: 836 118
PvuII CAG'CTG
Cuts at: 0 97 954
Size: 97 857
SfoI GGC'GCC
Cuts at: 0 791 954
Size: 791 163
SmlI C'TyrA_G
Cuts at: 0 148 415 954
Size: 148 267 539
Fragments arranged by size:
539 267 148
SphI G_CATG'C
Cuts at: 0 483 954
Size: 483 471
StuI AGG'CCT
Cuts at: 0 44 954
Size: 44 910
StyI C'CwwG_G
Cuts at: 0 54 832 954
Size: 54 778 122
Fragments arranged by size:
778 122 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 747 763 954
Size: 568 179 16 191
Fragments arranged by size:
568 191 179 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 954
Size: 298 32 624
Fragments arranged by size:
624 298 32
XmnI GAAnn'nnTTC
Cuts at: 0 44 954
Size: 44 910
Enzymes that do cut:
AarI AcuI AfeI AlfI AloI ApaI BanI BanII
BbeI BbvCI BclI Bme1580I BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstEII Bsu36I BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI
MslI MspA1I NaeI NarI NgoMIV NspI PfoI PpiI
PspOMI PvuII SfoI SmlI SphI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI