(Linear) (Six-base) MAPSORT of: KIBB7109.seq  Check: 805  from: 1  to: 744



KIBB7109.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AflII C'TTAA_G

Cuts at:      0     285     744 
   Size:        285     459
 
ApaI G_GGCC'C

Cuts at:      0     554     744 
   Size:        554     190
 
ApoI r'AATT_y

Cuts at:      0     493     744 
   Size:        493     251
 
BanII G_rGCy'C

Cuts at:      0     554     744 
   Size:        554     190
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     421     744 
   Size:        421     323
 
Bme1580I G_kGCm'C

Cuts at:      0     554     744 
   Size:        554     190
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     416     744 
   Size:        416     328
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     104     134     744 
   Size:        104      30     610

  Fragments arranged by size: 

                 610     104      30
 
BseYI C'CCAG_C

Cuts at:      0     580     637     744 
   Size:        580      57     107

  Fragments arranged by size: 

                 580     107      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     744 
   Size:        156     588
 
Bsp1286I G_dGCh'C

Cuts at:      0     554     744 
   Size:        554     190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     635     744 
   Size:        635     109
 
BsrDI GCAATG_nn'

Cuts at:      0     519     744 
   Size:        519     225
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     334     744 
   Size:        334     410
 
BtsI GCAGTG_nn'

Cuts at:      0     341     744 
   Size:        341     403
 
DraI TTT'AAA

Cuts at:      0     704     744 
   Size:        704      40
 
EaeI y'GGCC_r

Cuts at:      0     647     744 
   Size:        647      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0     551     744 
   Size:        551     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     287     319     385     417     744 
   Size:        287      32      66      32     327

  Fragments arranged by size: 

                 327     287      66      32      32
 
HincII GTy'rAC

Cuts at:      0      79     744 
   Size:         79     665
 
MscI TGG'CCA

Cuts at:      0     649     744 
   Size:        649      95
 
MspA1I CmG'CkG

Cuts at:      0      73     744 
   Size:         73     671
 
NspI r_CATG'y

Cuts at:      0     436     656     744 
   Size:        436     220      88

  Fragments arranged by size: 

                 436     220      88
 
PspOMI G'GGCC_C

Cuts at:      0     550     744 
   Size:        550     194
 
PvuII CAG'CTG

Cuts at:      0      73     744 
   Size:         73     671
 
SmlI C'TyrA_G

Cuts at:      0     285     744 
   Size:        285     459
 
SspI AAT'ATT

Cuts at:      0     198     744 
   Size:        198     546
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     435     744 
   Size:        435     309
 

 Enzymes that do cut:

    AflII     ApaI     ApoI    BanII     BbsI Bme1580I     BmrI    BsaXI
    BseYI     BsgI Bsp1286I    BspMI    BsrDI   BstAPI     BtsI     DraI
     EaeI EcoO109I    Hin4I   HincII     MscI   MspA1I     NspI   PspOMI
    PvuII     SmlI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI