Restriction map for
pF1KB6987
(Linear) (Six-base) MAPSORT of: KIBB6987.seq Check: 3695 from: 1 to: 657
KIBB6987.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 455 657
Size: 37 418 202
Fragments arranged by size:
418 202 37
AlwNI CAG_nnn'CTG
Cuts at: 0 226 412 657
Size: 226 186 245
Fragments arranged by size:
245 226 186
ApaI G_GGCC'C
Cuts at: 0 447 657
Size: 447 210
ApoI r'AATT_y
Cuts at: 0 266 657
Size: 266 391
AvaI C'yCGr_G
Cuts at: 0 36 110 657
Size: 36 74 547
Fragments arranged by size:
547 74 36
BanII G_rGCy'C
Cuts at: 0 119 404 447 650 657
Size: 119 285 43 203 7
Fragments arranged by size:
285 203 119 43 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 237 271 657
Size: 237 34 386
Fragments arranged by size:
386 237 34
Bme1580I G_kGCm'C
Cuts at: 0 447 657
Size: 447 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 341 419 519 657
Size: 341 78 100 138
Fragments arranged by size:
341 138 100 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 657
Size: 562 95
Bpu10I CC'TnA_GC
Cuts at: 0 296 657
Size: 296 361
BsaWI w'CCGG_w
Cuts at: 0 68 657
Size: 68 589
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 76 344 479 657
Size: 73 3 268 135 178
Fragments arranged by size:
268 178 135 73 3
BseYI C'CCAG_C
Cuts at: 0 462 657
Size: 462 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 657
Size: 398 259
BsiHKAI G_wGCw'C
Cuts at: 0 119 404 650 657
Size: 119 285 246 7
Fragments arranged by size:
285 246 119 7
BsmI GAATG_Cn'
Cuts at: 0 240 657
Size: 240 417
Bsp1286I G_dGCh'C
Cuts at: 0 119 404 447 650 657
Size: 119 285 43 203 7
Fragments arranged by size:
285 203 119 43 7
BspEI T'CCGG_A
Cuts at: 0 68 657
Size: 68 589
BsrDI GCAATG_nn'
Cuts at: 0 380 657
Size: 380 277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 226 235 657
Size: 226 9 422
Fragments arranged by size:
422 226 9
Bsu36I CC'TnA_GG
Cuts at: 0 539 657
Size: 539 118
BtsI GCAGTG_nn'
Cuts at: 0 514 657
Size: 514 143
EaeI y'GGCC_r
Cuts at: 0 314 657
Size: 314 343
EcoICRI GAG'CTC
Cuts at: 0 117 402 648 657
Size: 117 285 246 9
Fragments arranged by size:
285 246 117 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 455 562 657
Size: 37 418 107 95
Fragments arranged by size:
418 107 95 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 657
Size: 55 602
EcoO109I rG'GnC_Cy
Cuts at: 0 444 657
Size: 444 213
EcoRV GAT'ATC
Cuts at: 0 102 657
Size: 102 555
HaeII r_GCGC'y
Cuts at: 0 478 657
Size: 478 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 657
Size: 196 32 429
Fragments arranged by size:
429 196 32
MscI TGG'CCA
Cuts at: 0 316 657
Size: 316 341
MslI CAynn'nnrTG
Cuts at: 0 310 657
Size: 310 347
MspA1I CmG'CkG
Cuts at: 0 466 657
Size: 466 191
NdeI CA'TA_TG
Cuts at: 0 396 657
Size: 396 261
NspI r_CATG'y
Cuts at: 0 523 657
Size: 523 134
PsiI TTA'TAA
Cuts at: 0 163 657
Size: 163 494
PspOMI G'GGCC_C
Cuts at: 0 443 657
Size: 443 214
PstI C_TGCA'G
Cuts at: 0 633 657
Size: 633 24
SacI G_AGCT'C
Cuts at: 0 119 404 650 657
Size: 119 285 246 7
Fragments arranged by size:
285 246 119 7
ScaI AGT'ACT
Cuts at: 0 351 657
Size: 351 306
SfcI C'TryA_G
Cuts at: 0 185 629 657
Size: 185 444 28
Fragments arranged by size:
444 185 28
SphI G_CATG'C
Cuts at: 0 523 657
Size: 523 134
TatI w'GTAC_w
Cuts at: 0 349 657
Size: 349 308
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BcgI Bme1580I
BmrI BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BsrDI BstAPI Bsu36I BtsI EaeI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I MscI
MslI MspA1I NdeI NspI PsiI PspOMI PstI SacI
ScaI SfcI SphI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI