(Linear) (Six-base) MAPSORT of: KIBB6977.seq  Check: 1786  from: 1  to: 651



KIBB6977.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AatII G_ACGT'C

Cuts at:      0      22     651 
   Size:         22     629
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     651 
   Size:        558      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     331     651 
   Size:        331     320
 
BanI G'GyrC_C

Cuts at:      0     388     651 
   Size:        388     263
 
BanII G_rGCy'C

Cuts at:      0     344     651 
   Size:        344     307
 
BbeI G_GCGC'C

Cuts at:      0     392     651 
   Size:        392     259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     357     651 
   Size:        357     294
 
BglII A'GATC_T

Cuts at:      0     488     651 
   Size:        488     163
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     491     651 
   Size:        491     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     651 
   Size:        358     293
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     651 
   Size:        532     119
 
BsaHI Gr'CG_yC

Cuts at:      0      19     389     651 
   Size:         19     370     262

  Fragments arranged by size: 

                 370     262      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     367     637     651 
   Size:        121     246     270      14

  Fragments arranged by size: 

                 270     246     121      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     651 
   Size:        344     307
 
BsrBI CCG'CTC

Cuts at:      0     626     651 
   Size:        626      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     378     651 
   Size:        378     273
 
BstYI r'GATC_y

Cuts at:      0     488     651 
   Size:        488     163
 
Bsu36I CC'TnA_GG

Cuts at:      0     355     651 
   Size:        355     296
 
BtsI GCAGTG_nn'

Cuts at:      0      78     625     651 
   Size:         78     547      26

  Fragments arranged by size: 

                 547      78      26
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     403     651 
   Size:        403     248
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     558     651 
   Size:        358     200      93

  Fragments arranged by size: 

                 358     200      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      96     264     418     651 
   Size:         96     168     154     233

  Fragments arranged by size: 

                 233     168     154      96
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423     651 
   Size:        423     228
 
FspI TGC'GCA

Cuts at:      0     610     651 
   Size:        610      41
 
HaeII r_GCGC'y

Cuts at:      0     392     651 
   Size:        392     259
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     508     540     651 
   Size:        508      32     111

  Fragments arranged by size: 

                 508     111      32
 
KasI G'GCGC_C

Cuts at:      0     388     651 
   Size:        388     263
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     287     314     651 
   Size:        277      10      27     337

  Fragments arranged by size: 

                 337     277      27      10
 
MslI CAynn'nnrTG

Cuts at:      0     533     651 
   Size:        533     118
 
MspA1I CmG'CkG

Cuts at:      0     144     641     651 
   Size:        144     497      10

  Fragments arranged by size: 

                 497     144      10
 
NarI GG'CG_CC

Cuts at:      0     389     651 
   Size:        389     262
 
PfoI T'CCnGG_A

Cuts at:      0      13     651 
   Size:         13     638
 
PpuMI rG'GwC_Cy

Cuts at:      0     423     651 
   Size:        423     228
 
PstI C_TGCA'G

Cuts at:      0     215     651 
   Size:        215     436
 
PvuII CAG'CTG

Cuts at:      0     144     641     651 
   Size:        144     497      10

  Fragments arranged by size: 

                 497     144      10
 
SfcI C'TryA_G

Cuts at:      0       5     211     504     651 
   Size:          5     206     293     147

  Fragments arranged by size: 

                 293     206     147       5
 
SfoI GGC'GCC

Cuts at:      0     390     651 
   Size:        390     261
 
SmlI C'TyrA_G

Cuts at:      0     547     651 
   Size:        547     104
 
Tth111I GACn'n_nGTC

Cuts at:      0     592     651 
   Size:        592      59
 
ZraI GAC'GTC

Cuts at:      0      20     651 
   Size:         20     631
 

 Enzymes that do cut:

    AatII     AcuI     AlfI     BanI    BanII     BbeI     BbsI    BglII
     BmrI     BpmI    BpuEI    BsaHI     BsgI Bsp1286I    BsrBI    BstXI
    BstYI   Bsu36I     BtsI     DrdI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NarI     PfoI
    PpuMI     PstI    PvuII     SfcI     SfoI     SmlI  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI