(Linear) (Six-base) MAPSORT of: KIBB6974.seq  Check: 9980  from: 1  to: 729



KIBB6974.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AatII G_ACGT'C

Cuts at:      0     115     729 
   Size:        115     614
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     729 
   Size:        293     436
 
ApoI r'AATT_y

Cuts at:      0     447     729 
   Size:        447     282
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     419     452     729 
   Size:        419      33     277

  Fragments arranged by size: 

                 419     277      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      64     143     416     688     729 
   Size:         55       9      79     273     272      41

  Fragments arranged by size: 

                 273     272      79      55      41       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     729 
   Size:        139     590
 
BsaBI GATnn'nnATC

Cuts at:      0     572     729 
   Size:        572     157
 
BsaHI Gr'CG_yC

Cuts at:      0     112     729 
   Size:        112     617
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     729 
   Size:         46      30     653

  Fragments arranged by size: 

                 653      46      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     729 
   Size:         31     698
 
BseYI C'CCAG_C

Cuts at:      0      91     729 
   Size:         91     638
 
BtgI C'CryG_G

Cuts at:      0     423     729 
   Size:        423     306
 
DraI TTT'AAA

Cuts at:      0     251     729 
   Size:        251     478
 
EarI CTCTTCn'nnn_

Cuts at:      0     357     729 
   Size:        357     372
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      64     143     293     416     688     729 
   Size:         55       9      79     150     123     272      41

  Fragments arranged by size: 

                 272     150     123      79      55      41       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     729 
   Size:         39     690
 
EcoRV GAT'ATC

Cuts at:      0     591     729 
   Size:        591     138
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     211     298     729 
   Size:        211      87     431

  Fragments arranged by size: 

                 431     211      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     729 
   Size:        288      32     409

  Fragments arranged by size: 

                 409     288      32
 
MfeI C'AATT_G

Cuts at:      0     440     729 
   Size:        440     289
 
MslI CAynn'nnrTG

Cuts at:      0     106     729 
   Size:        106     623
 
MspA1I CmG'CkG

Cuts at:      0     316     724     729 
   Size:        316     408       5

  Fragments arranged by size: 

                 408     316       5
 
NcoI C'CATG_G

Cuts at:      0     423     729 
   Size:        423     306
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     600     651     729 
   Size:        600      51      78

  Fragments arranged by size: 

                 600      78      51
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     625     657     729 
   Size:        625      32      72

  Fragments arranged by size: 

                 625      72      32
 
PvuII CAG'CTG

Cuts at:      0     316     724     729 
   Size:        316     408       5

  Fragments arranged by size: 

                 408     316       5
 
SmlI C'TyrA_G

Cuts at:      0     154     729 
   Size:        154     575
 
SspI AAT'ATT

Cuts at:      0     206     729 
   Size:        206     523
 
StyI C'CwwG_G

Cuts at:      0     423     729 
   Size:        423     306
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     105     729 
   Size:        105     624
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     605     637     729 
   Size:        605      32      92

  Fragments arranged by size: 

                 605      92      32
 
XbaI T'CTAG_A

Cuts at:      0     222     729 
   Size:        222     507
 
ZraI GAC'GTC

Cuts at:      0     113     729 
   Size:        113     616
 

 Enzymes that do cut:

    AatII     AcuI     ApoI     BaeI     BpmI    BpuEI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BtgI     DraI     EarI  Eco57MI EcoO109I
    EcoRV     FalI    Hin4I     MfeI     MslI   MspA1I     NcoI    PflMI
     PpiI    PvuII     SmlI     SspI     StyI    TaqII     TstI     XbaI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI  Tth111I     XcmI
     XhoI     XmaI     XmnI