(Linear) (Six-base) MAPSORT of: KIBB6968.seq  Check: 9034  from: 1  to: 723



KIBB6968.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     622     723 
   Size:        622     101
 
ApoI r'AATT_y

Cuts at:      0       3     723 
   Size:          3     720
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     258     723 
   Size:        258     465
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     290     365     723 
   Size:        290      75     358

  Fragments arranged by size: 

                 358     290      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     723 
   Size:        394     329
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      13     723 
   Size:         13     710
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     182     212     723 
   Size:        182      30     511

  Fragments arranged by size: 

                 511     182      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     403     723 
   Size:        403     320
 
BtgI C'CryG_G

Cuts at:      0      23     723 
   Size:         23     700
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     387     422     723 
   Size:        387      35     301

  Fragments arranged by size: 

                 387     301      35
 
EaeI y'GGCC_r

Cuts at:      0     216     723 
   Size:        216     507
 
EarI CTCTTCn'nnn_

Cuts at:      0     317     479     491     723 
   Size:        317     162      12     232

  Fragments arranged by size: 

                 317     232     162      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     345     723 
   Size:        345     378
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     723 
   Size:         89     634
 
EcoRI G'AATT_C

Cuts at:      0       3     723 
   Size:          3     720
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     560     723 
   Size:        560     163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     490     522     580     612     723 
   Size:        154      32     304      32      58      32     111

  Fragments arranged by size: 

                 304     154     111      58      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     208     723 
   Size:        208     515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     168     723 
   Size:         94      74     555

  Fragments arranged by size: 

                 555      94      74
 
NcoI C'CATG_G

Cuts at:      0      23     723 
   Size:         23     700
 
NdeI CA'TA_TG

Cuts at:      0     143     723 
   Size:        143     580
 
PfoI T'CCnGG_A

Cuts at:      0     344     723 
   Size:        344     379
 
SexAI A'CCwGG_T

Cuts at:      0     447     723 
   Size:        447     276
 
SmlI C'TyrA_G

Cuts at:      0     373     723 
   Size:        373     350
 
StuI AGG'CCT

Cuts at:      0      15     723 
   Size:         15     708
 
StyI C'CwwG_G

Cuts at:      0      23      57     723 
   Size:         23      34     666

  Fragments arranged by size: 

                 666      34      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     192     723 
   Size:        192     531
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214     723 
   Size:        182      32     509

  Fragments arranged by size: 

                 509     182      32
 
XbaI T'CTAG_A

Cuts at:      0     547     723 
   Size:        547     176
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     352     723 
   Size:        352     371
 

 Enzymes that do cut:

    AlwNI     ApoI    BciVI     BmrI    BpuEI     BsaI    BsaXI    BspMI
     BtgI    CspCI     EaeI     EarI     EciI EcoO109I    EcoRI     FalI
    Hin4I  HindIII     MmeI     NcoI     NdeI     PfoI    SexAI     SmlI
     StuI     StyI    TaqII     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI  Tth111I     XhoI     XmaI     XmnI     ZraI