Restriction map for
pF1KB6959
(Linear) (Six-base) MAPSORT of: KIBB6959.seq Check: 4077 from: 1 to: 639
KIBB6959.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
Acc65I G'GTAC_C
Cuts at: 0 218 639
Size: 218 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 362 639
Size: 340 22 277
Fragments arranged by size:
340 277 22
ApaI G_GGCC'C
Cuts at: 0 264 639
Size: 264 375
AvaI C'yCGr_G
Cuts at: 0 395 639
Size: 395 244
BanI G'GyrC_C
Cuts at: 0 169 218 639
Size: 169 49 421
Fragments arranged by size:
421 169 49
BanII G_rGCy'C
Cuts at: 0 264 329 639
Size: 264 65 310
Fragments arranged by size:
310 264 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 27 79 639
Size: 27 52 560
Fragments arranged by size:
560 52 27
BbvCI CC'TCA_GC
Cuts at: 0 385 462 639
Size: 385 77 177
Fragments arranged by size:
385 177 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 639
Size: 114 34 491
Fragments arranged by size:
491 114 34
BglII A'GATC_T
Cuts at: 0 191 639
Size: 191 448
Bme1580I G_kGCm'C
Cuts at: 0 172 264 407 585 639
Size: 172 92 143 178 54
Fragments arranged by size:
178 172 143 92 54
BmtI G_CTAG'C
Cuts at: 0 493 639
Size: 493 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 475 639
Size: 232 243 164
Fragments arranged by size:
243 232 164
Bpu10I CC'TnA_GC
Cuts at: 0 385 462 639
Size: 385 77 177
Fragments arranged by size:
385 177 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 639
Size: 451 188
BsaWI w'CCGG_w
Cuts at: 0 554 639
Size: 554 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 639
Size: 427 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 639
Size: 272 367
BsiEI CG_ry'CG
Cuts at: 0 353 639
Size: 353 286
BsiHKAI G_wGCw'C
Cuts at: 0 329 639
Size: 329 310
Bsp1286I G_dGCh'C
Cuts at: 0 172 264 329 407 585 639
Size: 172 92 65 78 178 54
Fragments arranged by size:
178 172 92 78 65 54
BspEI T'CCGG_A
Cuts at: 0 554 639
Size: 554 85
BsrBI CCG'CTC
Cuts at: 0 216 639
Size: 216 423
BsrDI GCAATG_nn'
Cuts at: 0 108 639
Size: 108 531
BssSI C'ACGA_G
Cuts at: 0 364 639
Size: 364 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 639
Size: 573 66
BstYI r'GATC_y
Cuts at: 0 191 639
Size: 191 448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 639
Size: 327 312
BtsI GCAGTG_nn'
Cuts at: 0 260 585 639
Size: 260 325 54
Fragments arranged by size:
325 260 54
EarI CTCTTCn'nnn_
Cuts at: 0 454 610 639
Size: 454 156 29
Fragments arranged by size:
454 156 29
EcoICRI GAG'CTC
Cuts at: 0 327 639
Size: 327 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 340 362 475 639
Size: 232 108 22 113 164
Fragments arranged by size:
232 164 113 108 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 639
Size: 388 251
EcoO109I rG'GnC_Cy
Cuts at: 0 260 261 620 639
Size: 260 1 359 19
Fragments arranged by size:
359 260 19 1
KpnI G_GTAC'C
Cuts at: 0 222 639
Size: 222 417
MslI CAynn'nnrTG
Cuts at: 0 160 364 639
Size: 160 204 275
Fragments arranged by size:
275 204 160
MspA1I CmG'CkG
Cuts at: 0 175 205 639
Size: 175 30 434
Fragments arranged by size:
434 175 30
NheI G'CTAG_C
Cuts at: 0 489 639
Size: 489 150
PasI CC'CwG_GG
Cuts at: 0 607 639
Size: 607 32
PfoI T'CCnGG_A
Cuts at: 0 452 639
Size: 452 187
PsiI TTA'TAA
Cuts at: 0 26 639
Size: 26 613
PspOMI G'GGCC_C
Cuts at: 0 260 639
Size: 260 379
PvuI CG_AT'CG
Cuts at: 0 353 639
Size: 353 286
PvuII CAG'CTG
Cuts at: 0 205 639
Size: 205 434
SacI G_AGCT'C
Cuts at: 0 329 639
Size: 329 310
SapI GCTCTTCn'nnn_
Cuts at: 0 454 639
Size: 454 185
SmaI CCC'GGG
Cuts at: 0 397 639
Size: 397 242
XmaI C'CCGG_G
Cuts at: 0 395 639
Size: 395 244
Enzymes that do cut:
Acc65I AcuI ApaI AvaI BanI BanII BbsI BbvCI
BcgI BglII Bme1580I BmtI BpmI Bpu10I BsaI BsaWI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrDI
BssSI BstXI BstYI BtgZI BtsI EarI EcoICRI Eco57MI
EcoNI EcoO109I KpnI MslI MspA1I NheI PasI PfoI
PsiI PspOMI PvuI PvuII SacI SapI SmaI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BplI BpuEI BsaAI
BsaBI BsaHI BsaXI BseYI BsiWI BsmI BsmBI BspHI
BspMI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI