(Linear) (Six-base) MAPSORT of: KIBB6947.seq  Check: 6025  from: 1  to: 633



KIBB6947.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AccI GT'mk_AC

Cuts at:      0     530     633 
   Size:        530     103
 
AleI CACnn'nnGTG

Cuts at:      0     234     633 
   Size:        234     399
 
ApoI r'AATT_y

Cuts at:      0     150     310     331     577     633 
   Size:        150     160      21     246      56

  Fragments arranged by size: 

                 246     160     150      56      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     130     356     633 
   Size:        130     226     277

  Fragments arranged by size: 

                 277     226     130
 
BmtI G_CTAG'C

Cuts at:      0     265     633 
   Size:        265     368
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     567     633 
   Size:        567      66
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     633 
   Size:         94     539
 
BsmI GAATG_Cn'

Cuts at:      0     286     633 
   Size:        286     347
 
BsrFI r'CCGG_y

Cuts at:      0     178     633 
   Size:        178     455
 
BstZ17I GTA'TAC

Cuts at:      0     531     633 
   Size:        531     102
 
Bsu36I CC'TnA_GG

Cuts at:      0     221     633 
   Size:        221     412
 
BtgI C'CryG_G

Cuts at:      0      44     633 
   Size:         44     589
 
BtsI GCAGTG_nn'

Cuts at:      0      69     633 
   Size:         69     564
 
DraI TTT'AAA

Cuts at:      0     113     302     633 
   Size:        113     189     331

  Fragments arranged by size: 

                 331     189     113
 
EaeI y'GGCC_r

Cuts at:      0      66     633 
   Size:         66     567
 
EcoRI G'AATT_C

Cuts at:      0     310     331     577     633 
   Size:        310      21     246      56

  Fragments arranged by size: 

                 310     246      56      21
 
HindIII A'AGCT_T

Cuts at:      0     200     633 
   Size:        200     433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     633 
   Size:        287     346
 
MscI TGG'CCA

Cuts at:      0      68     633 
   Size:         68     565
 
MslI CAynn'nnrTG

Cuts at:      0     234     633 
   Size:        234     399
 
MspA1I CmG'CkG

Cuts at:      0     234     633 
   Size:        234     399
 
NcoI C'CATG_G

Cuts at:      0      44     633 
   Size:         44     589
 
NheI G'CTAG_C

Cuts at:      0     261     633 
   Size:        261     372
 
PstI C_TGCA'G

Cuts at:      0      40     633 
   Size:         40     593
 
PvuII CAG'CTG

Cuts at:      0     234     633 
   Size:        234     399
 
SfcI C'TryA_G

Cuts at:      0      36     633 
   Size:         36     597
 
SspI AAT'ATT

Cuts at:      0     327     633 
   Size:        327     306
 
StyI C'CwwG_G

Cuts at:      0      44     633 
   Size:         44     589
 

 Enzymes that do cut:

     AccI     AleI     ApoI     BbsI     BmtI     BplI    BseRI     BsmI
    BsrFI  BstZ17I   Bsu36I     BtgI     BtsI     DraI     EaeI    EcoRI
  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NheI     PstI
    PvuII     SfcI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI