Restriction map for
pF1KB6940
(Linear) (Six-base) MAPSORT of: KIBB6940.seq Check: 5786 from: 1 to: 687
KIBB6940.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 334 687
Size: 334 353
AflIII A'CryG_T
Cuts at: 0 237 569 687
Size: 237 332 118
Fragments arranged by size:
332 237 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 640 687
Size: 630 10 47
Fragments arranged by size:
630 47 10
AlwNI CAG_nnn'CTG
Cuts at: 0 516 687
Size: 516 171
ApaLI G'TGCA_C
Cuts at: 0 538 687
Size: 538 149
ApoI r'AATT_y
Cuts at: 0 207 687
Size: 207 480
AvaI C'yCGr_G
Cuts at: 0 422 687
Size: 422 265
BanI G'GyrC_C
Cuts at: 0 550 687
Size: 550 137
BciVI GTATCCnnnnn_n'
Cuts at: 0 513 687
Size: 513 174
BclI T'GATC_A
Cuts at: 0 635 687
Size: 635 52
BglII A'GATC_T
Cuts at: 0 611 687
Size: 611 76
Bme1580I G_kGCm'C
Cuts at: 0 542 687
Size: 542 145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 687
Size: 289 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 687
Size: 463 224
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 679 687
Size: 466 213 8
Fragments arranged by size:
466 213 8
BseYI C'CCAG_C
Cuts at: 0 21 687
Size: 21 666
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 529 687
Size: 169 360 158
Fragments arranged by size:
360 169 158
BsiHKAI G_wGCw'C
Cuts at: 0 542 687
Size: 542 145
Bsp1286I G_dGCh'C
Cuts at: 0 542 687
Size: 542 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 334 687
Size: 334 353
BsrDI GCAATG_nn'
Cuts at: 0 680 687
Size: 680 7
BsrGI T'GTAC_A
Cuts at: 0 363 687
Size: 363 324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 687
Size: 156 531
BstYI r'GATC_y
Cuts at: 0 611 687
Size: 611 76
Bsu36I CC'TnA_GG
Cuts at: 0 284 662 687
Size: 284 378 25
Fragments arranged by size:
378 284 25
BtgI C'CryG_G
Cuts at: 0 143 687
Size: 143 544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 687
Size: 289 398
HindIII A'AGCT_T
Cuts at: 0 496 687
Size: 496 191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 687
Size: 629 58
NcoI C'CATG_G
Cuts at: 0 143 687
Size: 143 544
NspI r_CATG'y
Cuts at: 0 40 241 573 687
Size: 40 201 332 114
Fragments arranged by size:
332 201 114 40
PciI A'CATG_T
Cuts at: 0 237 569 687
Size: 237 332 118
Fragments arranged by size:
332 237 118
PfoI T'CCnGG_A
Cuts at: 0 515 687
Size: 515 172
PspXI vC'TCGA_Gb
Cuts at: 0 422 687
Size: 422 265
SmlI C'TyrA_G
Cuts at: 0 422 478 687
Size: 422 56 209
Fragments arranged by size:
422 209 56
SphI G_CATG'C
Cuts at: 0 40 687
Size: 40 647
SspI AAT'ATT
Cuts at: 0 276 687
Size: 276 411
StyI C'CwwG_G
Cuts at: 0 6 143 461 687
Size: 6 137 318 226
Fragments arranged by size:
318 226 137 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 687
Size: 80 607
TatI w'GTAC_w
Cuts at: 0 123 363 687
Size: 123 240 324
Fragments arranged by size:
324 240 123
XhoI C'TCGA_G
Cuts at: 0 422 687
Size: 422 265
XmnI GAAnn'nnTTC
Cuts at: 0 50 687
Size: 50 637
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApaLI ApoI AvaI BanI
BciVI BclI BglII Bme1580I BpmI BpuEI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstAPI BstYI
Bsu36I BtgI Eco57MI HindIII MmeI NcoI NspI PciI
PfoI PspXI SmlI SphI SspI StyI TaqII TatI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XmaI ZraI