Restriction map for
pF1KB6936
(Linear) (Six-base) MAPSORT of: KIBB6936.seq Check: 2116 from: 1 to: 687
KIBB6936.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
Acc65I G'GTAC_C
Cuts at: 0 116 687
Size: 116 571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 687
Size: 508 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 687
Size: 370 317
ApaI G_GGCC'C
Cuts at: 0 71 545 687
Size: 71 474 142
Fragments arranged by size:
474 142 71
AvaI C'yCGr_G
Cuts at: 0 92 215 454 584 687
Size: 92 123 239 130 103
Fragments arranged by size:
239 130 123 103 92
BamHI G'GATC_C
Cuts at: 0 497 687
Size: 497 190
BanI G'GyrC_C
Cuts at: 0 116 687
Size: 116 571
BanII G_rGCy'C
Cuts at: 0 71 340 545 687
Size: 71 269 205 142
Fragments arranged by size:
269 205 142 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 333 367 687
Size: 333 34 320
Fragments arranged by size:
333 320 34
BglII A'GATC_T
Cuts at: 0 476 687
Size: 476 211
BlpI GC'TnA_GC
Cuts at: 0 422 687
Size: 422 265
Bme1580I G_kGCm'C
Cuts at: 0 71 545 687
Size: 71 474 142
Fragments arranged by size:
474 142 71
BmgBI CAC'GTC
Cuts at: 0 557 687
Size: 557 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 498 687
Size: 498 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 377 669 687
Size: 324 53 292 18
Fragments arranged by size:
324 292 53 18
Bpu10I CC'TnA_GC
Cuts at: 0 409 614 687
Size: 409 205 73
Fragments arranged by size:
409 205 73
BsaAI yAC'GTr
Cuts at: 0 194 306 687
Size: 194 112 381
Fragments arranged by size:
381 194 112
BsaHI Gr'CG_yC
Cuts at: 0 247 687
Size: 247 440
BseYI C'CCAG_C
Cuts at: 0 72 687
Size: 72 615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 460 687
Size: 249 211 227
Fragments arranged by size:
249 227 211
BsiEI CG_ry'CG
Cuts at: 0 125 687
Size: 125 562
BsiHKAI G_wGCw'C
Cuts at: 0 311 374 687
Size: 311 63 313
Fragments arranged by size:
313 311 63
Bsp1286I G_dGCh'C
Cuts at: 0 71 311 340 374 545 687
Size: 71 240 29 34 171 142
Fragments arranged by size:
240 171 142 71 34 29
BsrDI GCAATG_nn'
Cuts at: 0 106 185 687
Size: 106 79 502
Fragments arranged by size:
502 106 79
BssHII G'CGCG_C
Cuts at: 0 523 687
Size: 523 164
BstYI r'GATC_y
Cuts at: 0 476 497 687
Size: 476 21 190
Fragments arranged by size:
476 190 21
Bsu36I CC'TnA_GG
Cuts at: 0 568 687
Size: 568 119
BtgI C'CryG_G
Cuts at: 0 128 293 687
Size: 128 165 394
Fragments arranged by size:
394 165 128
BtsI GCAGTG_nn'
Cuts at: 0 60 85 448 662 687
Size: 60 25 363 214 25
Fragments arranged by size:
363 214 60 25 25
DraIII CAC_nnn'GTG
Cuts at: 0 537 687
Size: 537 150
DrdI GACnn_nn'nnGTC
Cuts at: 0 252 687
Size: 252 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 377 508 669 687
Size: 324 53 131 161 18
Fragments arranged by size:
324 161 131 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 395 687
Size: 395 292
EcoO109I rG'GnC_Cy
Cuts at: 0 67 68 458 541 618 687
Size: 67 1 390 83 77 69
Fragments arranged by size:
390 83 77 69 67 1
HaeII r_GCGC'y
Cuts at: 0 429 687
Size: 429 258
KpnI G_GTAC'C
Cuts at: 0 120 687
Size: 120 567
MspA1I CmG'CkG
Cuts at: 0 41 227 263 285 687
Size: 41 186 36 22 402
Fragments arranged by size:
402 186 41 36 22
NcoI C'CATG_G
Cuts at: 0 293 687
Size: 293 394
PasI CC'CwG_GG
Cuts at: 0 332 687
Size: 332 355
PflMI CCAn_nnn'nTGG
Cuts at: 0 537 687
Size: 537 150
PfoI T'CCnGG_A
Cuts at: 0 139 687
Size: 139 548
PpuMI rG'GwC_Cy
Cuts at: 0 458 618 687
Size: 458 160 69
Fragments arranged by size:
458 160 69
PshAI GACnn'nnGTC
Cuts at: 0 650 687
Size: 650 37
PspOMI G'GGCC_C
Cuts at: 0 67 541 687
Size: 67 474 146
Fragments arranged by size:
474 146 67
PstI C_TGCA'G
Cuts at: 0 55 242 687
Size: 55 187 445
Fragments arranged by size:
445 187 55
PvuI CG_AT'CG
Cuts at: 0 125 687
Size: 125 562
PvuII CAG'CTG
Cuts at: 0 41 227 263 285 687
Size: 41 186 36 22 402
Fragments arranged by size:
402 186 41 36 22
ScaI AGT'ACT
Cuts at: 0 493 687
Size: 493 194
SfcI C'TryA_G
Cuts at: 0 51 238 433 687
Size: 51 187 195 254
Fragments arranged by size:
254 195 187 51
SmaI CCC'GGG
Cuts at: 0 94 217 456 687
Size: 94 123 239 231
Fragments arranged by size:
239 231 123 94
StyI C'CwwG_G
Cuts at: 0 293 687
Size: 293 394
TatI w'GTAC_w
Cuts at: 0 491 687
Size: 491 196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 687
Size: 675 12
XmaI C'CCGG_G
Cuts at: 0 92 215 454 687
Size: 92 123 239 233
Fragments arranged by size:
239 233 123 92
Enzymes that do cut:
Acc65I AcuI AlfI ApaI AvaI BamHI BanI BanII
BcgI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI
BssHII BstYI Bsu36I BtgI BtsI DraIII DrdI Eco57MI
EcoNI EcoO109I HaeII KpnI MspA1I NcoI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuI PvuII ScaI
SfcI SmaI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BmrI BmtI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI EaeI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI