Restriction map for
pF1KB6922
(Linear) (Six-base) MAPSORT of: KIBB6922.seq Check: 116 from: 1 to: 681
KIBB6922.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 681
Size: 315 366
AfeI AGC'GCT
Cuts at: 0 649 681
Size: 649 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 681
Size: 526 155
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 681
Size: 82 32 567
Fragments arranged by size:
567 82 32
AlwNI CAG_nnn'CTG
Cuts at: 0 418 681
Size: 418 263
ApaI G_GGCC'C
Cuts at: 0 77 681
Size: 77 604
AvaI C'yCGr_G
Cuts at: 0 166 427 681
Size: 166 261 254
Fragments arranged by size:
261 254 166
BanI G'GyrC_C
Cuts at: 0 672 681
Size: 672 9
BanII G_rGCy'C
Cuts at: 0 77 229 428 681
Size: 77 152 199 253
Fragments arranged by size:
253 199 152 77
BglI GCCn_nnn'nGGC
Cuts at: 0 16 233 535 681
Size: 16 217 302 146
Fragments arranged by size:
302 217 146 16
Bme1580I G_kGCm'C
Cuts at: 0 77 677 681
Size: 77 600 4
Fragments arranged by size:
600 77 4
BmgBI CAC'GTC
Cuts at: 0 207 681
Size: 207 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 367 514 681
Size: 241 126 147 167
Fragments arranged by size:
241 167 147 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 681
Size: 628 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 469 681
Size: 163 306 212
Fragments arranged by size:
306 212 163
BsaAI yAC'GTr
Cuts at: 0 541 681
Size: 541 140
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 445 681
Size: 409 36 236
Fragments arranged by size:
409 236 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 681
Size: 621 60
BsiEI CG_ry'CG
Cuts at: 0 11 681
Size: 11 670
Bsp1286I G_dGCh'C
Cuts at: 0 77 229 428 677 681
Size: 77 152 199 249 4
Fragments arranged by size:
249 199 152 77 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 560 621 681
Size: 560 61 60
Fragments arranged by size:
560 61 60
BsrBI CCG'CTC
Cuts at: 0 573 681
Size: 573 108
BsrFI r'CCGG_y
Cuts at: 0 10 114 150 681
Size: 10 104 36 531
Fragments arranged by size:
531 104 36 10
Bsu36I CC'TnA_GG
Cuts at: 0 230 681
Size: 230 451
BtsI GCAGTG_nn'
Cuts at: 0 136 681
Size: 136 545
DraIII CAC_nnn'GTG
Cuts at: 0 282 681
Size: 282 399
DrdI GACnn_nn'nnGTC
Cuts at: 0 397 681
Size: 397 284
EaeI y'GGCC_r
Cuts at: 0 8 152 681
Size: 8 144 529
Fragments arranged by size:
529 144 8
EagI C'GGCC_G
Cuts at: 0 8 681
Size: 8 673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 315 367 514 681
Size: 241 74 52 147 167
Fragments arranged by size:
241 167 147 74 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 681
Size: 556 125
EcoO109I rG'GnC_Cy
Cuts at: 0 73 74 681
Size: 73 1 607
Fragments arranged by size:
607 73 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 669 681
Size: 333 336 12
Fragments arranged by size:
336 333 12
HaeII r_GCGC'y
Cuts at: 0 651 669 681
Size: 651 18 12
Fragments arranged by size:
651 18 12
HincII GTy'rAC
Cuts at: 0 579 597 681
Size: 579 18 84
Fragments arranged by size:
579 84 18
MslI CAynn'nnrTG
Cuts at: 0 508 681
Size: 508 173
MspA1I CmG'CkG
Cuts at: 0 592 681
Size: 592 89
NaeI GCC'GGC
Cuts at: 0 12 116 152 681
Size: 12 104 36 529
Fragments arranged by size:
529 104 36 12
NgoMIV G'CCGG_C
Cuts at: 0 10 114 150 681
Size: 10 104 36 531
Fragments arranged by size:
531 104 36 10
PfoI T'CCnGG_A
Cuts at: 0 86 383 656 681
Size: 86 297 273 25
Fragments arranged by size:
297 273 86 25
PmlI CAC'GTG
Cuts at: 0 541 681
Size: 541 140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 681
Size: 88 32 561
Fragments arranged by size:
561 88 32
PspOMI G'GGCC_C
Cuts at: 0 73 681
Size: 73 608
PstI C_TGCA'G
Cuts at: 0 632 681
Size: 632 49
PvuII CAG'CTG
Cuts at: 0 592 681
Size: 592 89
SexAI A'CCwGG_T
Cuts at: 0 179 681
Size: 179 502
SfcI C'TryA_G
Cuts at: 0 628 681
Size: 628 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 16 681
Size: 16 665
SmlI C'TyrA_G
Cuts at: 0 166 643 681
Size: 166 477 38
Fragments arranged by size:
477 166 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 681
Size: 187 494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 681
Size: 191 32 458
Fragments arranged by size:
458 191 32
XhoI C'TCGA_G
Cuts at: 0 166 681
Size: 166 515
Enzymes that do cut:
AcuI AfeI AlfI AloI AlwNI ApaI AvaI BanI
BanII BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI
BseRI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI Bsu36I
BtsI DraIII DrdI EaeI EagI Eco57MI EcoNI EcoO109I
FalI HaeII HincII MslI MspA1I NaeI NgoMIV PfoI
PmlI PpiI PspOMI PstI PvuII SexAI SfcI SfiI
SmlI TaqII TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaBI
BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI
DraI EarI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI