(Linear) (Six-base) MAPSORT of: KIBB6908.seq  Check: 1741  from: 1  to: 672



KIBB6908.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     403     672 
   Size:        403     269
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     672 
   Size:        320     352
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     374     406     672 
   Size:        374      32     266

  Fragments arranged by size: 

                 374     266      32
 
ApoI r'AATT_y

Cuts at:      0     620     672 
   Size:        620      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     672 
   Size:        589      83
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     672 
   Size:        298     374
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     374     404     672 
   Size:        374      30     268

  Fragments arranged by size: 

                 374     268      30
 
BsiEI CG_ry'CG

Cuts at:      0      11     672 
   Size:         11     661
 
BstZ17I GTA'TAC

Cuts at:      0     404     672 
   Size:        404     268
 
BtgI C'CryG_G

Cuts at:      0      32     672 
   Size:         32     640
 
BtsI GCAGTG_nn'

Cuts at:      0      54     393     672 
   Size:         54     339     279

  Fragments arranged by size: 

                 339     279      54
 
EaeI y'GGCC_r

Cuts at:      0       8     149     672 
   Size:          8     141     523

  Fragments arranged by size: 

                 523     141       8
 
EagI C'GGCC_G

Cuts at:      0       8     672 
   Size:          8     664
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     672 
   Size:        296     376
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     589     672 
   Size:        320     269      83

  Fragments arranged by size: 

                 320     269      83
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313     672 
   Size:        313     359
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     189     672 
   Size:        189     483
 
FspI TGC'GCA

Cuts at:      0      46     672 
   Size:         46     626
 
HindIII A'AGCT_T

Cuts at:      0     265     672 
   Size:        265     407
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     477     672 
   Size:        477     195
 
MscI TGG'CCA

Cuts at:      0     151     672 
   Size:        151     521
 
MspA1I CmG'CkG

Cuts at:      0      21      42      57     672 
   Size:         21      21      15     615

  Fragments arranged by size: 

                 615      21      21      15
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     374     406     672 
   Size:        374      32     266

  Fragments arranged by size: 

                 374     266      32
 
PstI C_TGCA'G

Cuts at:      0      89     672 
   Size:         89     583
 
PvuII CAG'CTG

Cuts at:      0      42      57     672 
   Size:         42      15     615

  Fragments arranged by size: 

                 615      42      15
 
SfcI C'TryA_G

Cuts at:      0      85     672 
   Size:         85     587
 
SspI AAT'ATT

Cuts at:      0     444     672 
   Size:        444     228
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      23     672 
   Size:         23     649
 

 Enzymes that do cut:

     AccI     AcuI     AloI     ApoI     BpmI     BsaI    BsaXI    BsiEI
  BstZ17I     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI    EcoNI
     FalI     FspI  HindIII     MmeI     MscI   MspA1I     PpiI     PstI
    PvuII     SfcI     SspI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI