(Linear) (Six-base) MAPSORT of: KIBB6892.seq  Check: 9635  from: 1  to: 606



KIBB6892.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      55     606 
   Size:         55     551
 
AfeI AGC'GCT

Cuts at:      0     265     606 
   Size:        265     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0     543     553     606 
   Size:        543      10      53

  Fragments arranged by size: 

                 543      53      10
 
ApaI G_GGCC'C

Cuts at:      0     481     606 
   Size:        481     125
 
BanII G_rGCy'C

Cuts at:      0     446     481     606 
   Size:        446      35     125

  Fragments arranged by size: 

                 446     125      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     606 
   Size:         81     525
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     474     508     606 
   Size:        474      34      98

  Fragments arranged by size: 

                 474      98      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      48     606 
   Size:         48     558
 
BglI GCCn_nnn'nGGC

Cuts at:      0     328     606 
   Size:        328     278
 
Bme1580I G_kGCm'C

Cuts at:      0     481     606 
   Size:        481     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     165     606 
   Size:         59     106     441

  Fragments arranged by size: 

                 441     106      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     606 
   Size:        184     422
 
BsaHI Gr'CG_yC

Cuts at:      0     487     606 
   Size:        487     119
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     606 
   Size:         85     521
 
Bsp1286I G_dGCh'C

Cuts at:      0     446     481     606 
   Size:        446      35     125

  Fragments arranged by size: 

                 446     125      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      55     606 
   Size:         55     551
 
BsrFI r'CCGG_y

Cuts at:      0     370     606 
   Size:        370     236
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      51     606 
   Size:         51     555
 
Bsu36I CC'TnA_GG

Cuts at:      0     592     606 
   Size:        592      14
 
DraIII CAC_nnn'GTG

Cuts at:      0      67     606 
   Size:         67     539
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      85     606 
   Size:         85     521
 
EaeI y'GGCC_r

Cuts at:      0     320     368     606 
   Size:        320      48     238

  Fragments arranged by size: 

                 320     238      48
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     218     606 
   Size:        200      18     388

  Fragments arranged by size: 

                 388     200      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     606 
   Size:        184     422
 
EcoO109I rG'GnC_Cy

Cuts at:      0      97     159     606 
   Size:         97      62     447

  Fragments arranged by size: 

                 447      97      62
 
FseI GG_CCGG'CC

Cuts at:      0     374     606 
   Size:        374     232
 
HaeII r_GCGC'y

Cuts at:      0     267     544     606 
   Size:        267     277      62

  Fragments arranged by size: 

                 277     267      62
 
MscI TGG'CCA

Cuts at:      0     322     606 
   Size:        322     284
 
NaeI GCC'GGC

Cuts at:      0     372     606 
   Size:        372     234
 
NgoMIV G'CCGG_C

Cuts at:      0     370     606 
   Size:        370     236
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     155     606 
   Size:        155     451
 
PpuMI rG'GwC_Cy

Cuts at:      0      97     159     606 
   Size:         97      62     447

  Fragments arranged by size: 

                 447      97      62
 
PspOMI G'GGCC_C

Cuts at:      0     477     606 
   Size:        477     129
 
SanDI GG'GwC_CC

Cuts at:      0      97     606 
   Size:         97     509
 
StuI AGG'CCT

Cuts at:      0     521     600     606 
   Size:        521      79       6

  Fragments arranged by size: 

                 521      79       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      78     606 
   Size:         78     528
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     181     196     213     228     606 
   Size:        181      15      17      15     378

  Fragments arranged by size: 

                 378     181      17      15      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330     606 
   Size:        330     276
 

 Enzymes that do cut:

     AarI     AfeI    AlwNI     ApaI    BanII     BbsI     BcgI    BciVI
     BglI Bme1580I     BmrI     BpmI    BsaHI    BseRI Bsp1286I    BspMI
    BsrFI    BstXI   Bsu36I   DraIII     DrdI     EaeI     EarI  Eco57MI
 EcoO109I     FseI    HaeII     MscI     NaeI   NgoMIV    PflMI    PpuMI
   PspOMI    SanDI     StuI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI