(Linear) (Six-base) MAPSORT of: KIBB6887.seq  Check: 4960  from: 1  to: 549



KIBB6887.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     274     536     549 
   Size:        274     262      13

  Fragments arranged by size: 

                 274     262      13
 
ApaLI G'TGCA_C

Cuts at:      0     309     549 
   Size:        309     240
 
BanII G_rGCy'C

Cuts at:      0     266     440     549 
   Size:        266     174     109

  Fragments arranged by size: 

                 266     174     109
 
BglII A'GATC_T

Cuts at:      0     404     549 
   Size:        404     145
 
Bme1580I G_kGCm'C

Cuts at:      0     313     429     549 
   Size:        313     116     120

  Fragments arranged by size: 

                 313     120     116
 
BmgBI CAC'GTC

Cuts at:      0      96     549 
   Size:         96     453
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     469     549 
   Size:        469      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     549 
   Size:        280     269
 
Bpu10I CC'TnA_GC

Cuts at:      0      50     549 
   Size:         50     499
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484     549 
   Size:        454      30      65

  Fragments arranged by size: 

                 454      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     152     430     448     549 
   Size:        152     278      18     101

  Fragments arranged by size: 

                 278     152     101      18
 
BseYI C'CCAG_C

Cuts at:      0     197     225     549 
   Size:        197      28     324

  Fragments arranged by size: 

                 324     197      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     313     440     549 
   Size:        313     127     109

  Fragments arranged by size: 

                 313     127     109
 
Bsp1286I G_dGCh'C

Cuts at:      0     266     313     429     440     549 
   Size:        266      47     116      11     109

  Fragments arranged by size: 

                 266     116     109      47      11
 
BstYI r'GATC_y

Cuts at:      0     404     549 
   Size:        404     145
 
BtgI C'CryG_G

Cuts at:      0     512     549 
   Size:        512      37
 
EaeI y'GGCC_r

Cuts at:      0     488     549 
   Size:        488      61
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     549 
   Size:         22     527
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     367     549 
   Size:        367     182
 
EcoICRI GAG'CTC

Cuts at:      0     438     549 
   Size:        438     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     549 
   Size:        280     269
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     528     549 
   Size:        528      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     549 
   Size:        454      32      63

  Fragments arranged by size: 

                 454      63      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     549 
   Size:        234     315
 
MscI TGG'CCA

Cuts at:      0     490     549 
   Size:        490      59
 
MspA1I CmG'CkG

Cuts at:      0      75     497     549 
   Size:         75     422      52

  Fragments arranged by size: 

                 422      75      52
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     513     549 
   Size:        513      36
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     549 
   Size:        166      32     351

  Fragments arranged by size: 

                 351     166      32
 
PshAI GACnn'nnGTC

Cuts at:      0     455     549 
   Size:        455      94
 
PstI C_TGCA'G

Cuts at:      0      39     549 
   Size:         39     510
 
SacI G_AGCT'C

Cuts at:      0     440     549 
   Size:        440     109
 
SbfI CC_TGCA'GG

Cuts at:      0      39     549 
   Size:         39     510
 
SfcI C'TryA_G

Cuts at:      0      35     111     369     549 
   Size:         35      76     258     180

  Fragments arranged by size: 

                 258     180      76      35
 
StuI AGG'CCT

Cuts at:      0     336     549 
   Size:        336     213
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     549 
   Size:        121     428
 
TatI w'GTAC_w

Cuts at:      0     101     549 
   Size:        101     448
 
Tth111I GACn'n_nGTC

Cuts at:      0      94     549 
   Size:         94     455
 

 Enzymes that do cut:

    AlwNI    ApaLI    BanII    BglII Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BstYI     BtgI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    Hin4I     MmeI
     MscI   MspA1I    PflMI     PpiI    PshAI     PstI     SacI     SbfI
     SfcI     StuI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI