(Linear) (Six-base) MAPSORT of: KIBB6857.seq  Check: 9070  from: 1  to: 537



KIBB6857.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     363     537 
   Size:        334      29     174

  Fragments arranged by size: 

                 334     174      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     537 
   Size:        306     231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     283     502     537 
   Size:        262      21     219      35

  Fragments arranged by size: 

                 262     219      35      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259     379     537 
   Size:        259     120     158

  Fragments arranged by size: 

                 259     158     120
 
BsrDI GCAATG_nn'

Cuts at:      0      20     537 
   Size:         20     517
 
BssSI C'ACGA_G

Cuts at:      0     334     537 
   Size:        334     203
 
BstYI r'GATC_y

Cuts at:      0     248     537 
   Size:        248     289
 
EaeI y'GGCC_r

Cuts at:      0     150     537 
   Size:        150     387
 
EarI CTCTTCn'nnn_

Cuts at:      0     319     537 
   Size:        319     218
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     406     537 
   Size:        406     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     283     334     363     502     537 
   Size:        262      21      51      29     139      35

  Fragments arranged by size: 

                 262     139      51      35      29      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     537 
   Size:        352     185
 
FspI TGC'GCA

Cuts at:      0     502     537 
   Size:        502      35
 
MfeI C'AATT_G

Cuts at:      0     313     537 
   Size:        313     224
 
MscI TGG'CCA

Cuts at:      0     152     537 
   Size:        152     385
 
NspI r_CATG'y

Cuts at:      0     490     537 
   Size:        490      47
 
PstI C_TGCA'G

Cuts at:      0     273     473     537 
   Size:        273     200      64

  Fragments arranged by size: 

                 273     200      64
 
SfcI C'TryA_G

Cuts at:      0     269     469     537 
   Size:        269     200      68

  Fragments arranged by size: 

                 269     200      68
 
SphI G_CATG'C

Cuts at:      0     490     537 
   Size:        490      47
 
XmnI GAAnn'nnTTC

Cuts at:      0     347     537 
   Size:        347     190
 

 Enzymes that do cut:

     AcuI    AlwNI     BpmI    BseRI    BsrDI    BssSI    BstYI     EaeI
     EarI     EciI  Eco57MI    EcoNI     FspI     MfeI     MscI     NspI
     PstI     SfcI     SphI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI