(Linear) (Six-base) MAPSORT of: KIBB6843.seq  Check: 9902  from: 1  to: 525



KIBB6843.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     444     525 
   Size:        444      81
 
AvaI C'yCGr_G

Cuts at:      0      64     525 
   Size:         64     461
 
BanI G'GyrC_C

Cuts at:      0     129     525 
   Size:        129     396
 
BglI GCCn_nnn'nGGC

Cuts at:      0      53     358     525 
   Size:         53     305     167

  Fragments arranged by size: 

                 305     167      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     311     525 
   Size:        311     214
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511     525 
   Size:        481      30      14

  Fragments arranged by size: 

                 481      30      14
 
BseYI C'CCAG_C

Cuts at:      0     192     525 
   Size:        192     333
 
BsiEI CG_ry'CG

Cuts at:      0      14     525 
   Size:         14     511
 
BsmI GAATG_Cn'

Cuts at:      0     323     525 
   Size:        323     202
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     453     525 
   Size:        453      72
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     222     525 
   Size:        222     303
 
BstYI r'GATC_y

Cuts at:      0     326     525 
   Size:        326     199
 
Bsu36I CC'TnA_GG

Cuts at:      0     466     525 
   Size:        466      59
 
BtgI C'CryG_G

Cuts at:      0      30     133     525 
   Size:         30     103     392

  Fragments arranged by size: 

                 392     103      30
 
BtsI GCAGTG_nn'

Cuts at:      0     368     525 
   Size:        368     157
 
EaeI y'GGCC_r

Cuts at:      0      33     525 
   Size:         33     492
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     525 
   Size:        462      63
 
FspI TGC'GCA

Cuts at:      0     220     525 
   Size:        220     305
 
FspAI rTGC'GCAy

Cuts at:      0     220     525 
   Size:        220     305
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     318     330     350     362     525 
   Size:        318      12      20      12     163

  Fragments arranged by size: 

                 318     163      20      12      12
 
MslI CAynn'nnrTG

Cuts at:      0     220     316     525 
   Size:        220      96     209

  Fragments arranged by size: 

                 220     209      96
 
MspA1I CmG'CkG

Cuts at:      0     441     525 
   Size:        441      84
 
NcoI C'CATG_G

Cuts at:      0      30     133     525 
   Size:         30     103     392

  Fragments arranged by size: 

                 392     103      30
 
PpuMI rG'GwC_Cy

Cuts at:      0     462     525 
   Size:        462      63
 
PstI C_TGCA'G

Cuts at:      0      21     326     449     525 
   Size:         21     305     123      76

  Fragments arranged by size: 

                 305     123      76      21
 
PvuII CAG'CTG

Cuts at:      0     441     525 
   Size:        441      84
 
ScaI AGT'ACT

Cuts at:      0     275     525 
   Size:        275     250
 
SfcI C'TryA_G

Cuts at:      0      17     322     445     525 
   Size:         17     305     123      80

  Fragments arranged by size: 

                 305     123      80      17
 
StyI C'CwwG_G

Cuts at:      0      30     133     525 
   Size:         30     103     392

  Fragments arranged by size: 

                 392     103      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      49     525 
   Size:         49     476
 
TatI w'GTAC_w

Cuts at:      0     273     525 
   Size:        273     252
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI     BglI     BmrI    BsaXI    BseYI    BsiEI
     BsmI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
 EcoO109I     FspI    FspAI    Hin4I     MslI   MspA1I     NcoI    PpuMI
     PstI    PvuII     ScaI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI