(Linear) (Six-base) MAPSORT of: KIBB6839.seq  Check: 2434  from: 1  to: 516



KIBB6839.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     516 
   Size:        323     193
 
AleI CACnn'nnGTG

Cuts at:      0     304     516 
   Size:        304     212
 
ApoI r'AATT_y

Cuts at:      0     245     516 
   Size:        245     271
 
AvaI C'yCGr_G

Cuts at:      0     163     516 
   Size:        163     353
 
BanI G'GyrC_C

Cuts at:      0     454     516 
   Size:        454      62
 
BanII G_rGCy'C

Cuts at:      0     164     499     516 
   Size:        164     335      17

  Fragments arranged by size: 

                 335     164      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     320     374     516 
   Size:        320      54     142

  Fragments arranged by size: 

                 320     142      54
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     444     478     516 
   Size:        444      34      38

  Fragments arranged by size: 

                 444      38      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     327     454     516 
   Size:        327     127      62

  Fragments arranged by size: 

                 327     127      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     233     516 
   Size:        167      66     283

  Fragments arranged by size: 

                 283     167      66
 
BsaHI Gr'CG_yC

Cuts at:      0     371     516 
   Size:        371     145
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     109     164     181     184     220     516 
   Size:        106       3      55      17       3      36     296

  Fragments arranged by size: 

                 296     106      55      36      17       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     516 
   Size:        424      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     293     499     516 
   Size:        293     206      17

  Fragments arranged by size: 

                 293     206      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     293     499     516 
   Size:        164     129     206      17

  Fragments arranged by size: 

                 206     164     129      17
 
BssSI C'ACGA_G

Cuts at:      0     106     516 
   Size:        106     410
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     134     516 
   Size:        134     382
 
DraI TTT'AAA

Cuts at:      0     360     516 
   Size:        360     156
 
EaeI y'GGCC_r

Cuts at:      0     467     516 
   Size:        467      49
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     516 
   Size:        340     176
 
EcoICRI GAG'CTC

Cuts at:      0     497     516 
   Size:        497      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     233     323     516 
   Size:        167      66      90     193

  Fragments arranged by size: 

                 193     167      90      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     149     516 
   Size:        149     367
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     125     134     157     516 
   Size:        102      23       9      23     359

  Fragments arranged by size: 

                 359     102      23      23       9
 
HincII GTy'rAC

Cuts at:      0     333     516 
   Size:        333     183
 
HpaI GTT'AAC

Cuts at:      0     333     516 
   Size:        333     183
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     516 
   Size:        222     294
 
MscI TGG'CCA

Cuts at:      0     469     516 
   Size:        469      47
 
MslI CAynn'nnrTG

Cuts at:      0      69     304     379     516 
   Size:         69     235      75     137

  Fragments arranged by size: 

                 235     137      75      69
 
NspI r_CATG'y

Cuts at:      0      68     516 
   Size:         68     448
 
PshAI GACnn'nnGTC

Cuts at:      0     280     516 
   Size:        280     236
 
PstI C_TGCA'G

Cuts at:      0     389     516 
   Size:        389     127
 
SacI G_AGCT'C

Cuts at:      0     499     516 
   Size:        499      17
 
SfcI C'TryA_G

Cuts at:      0     385     516 
   Size:        385     131
 
SmlI C'TyrA_G

Cuts at:      0      10     516 
   Size:         10     506
 
SphI G_CATG'C

Cuts at:      0      68     516 
   Size:         68     448
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     AvaI     BanI    BanII     BbsI     BcgI
     BmrI     BpmI    BsaHI    BseRI     BsgI  BsiHKAI Bsp1286I    BssSI
    BstXI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI    Hin4I
   HincII     HpaI     MmeI     MscI     MslI     NspI    PshAI     PstI
     SacI     SfcI     SmlI     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI