(Linear) (Six-base) MAPSORT of: KIBB6837.seq  Check: 9376  from: 1  to: 516



KIBB6837.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     509     516 
   Size:        509       7
 
ApaI G_GGCC'C

Cuts at:      0      36     516 
   Size:         36     480
 
ApoI r'AATT_y

Cuts at:      0     271     516 
   Size:        271     245
 
AseI AT'TA_AT

Cuts at:      0     302     516 
   Size:        302     214
 
BanII G_rGCy'C

Cuts at:      0      36     516 
   Size:         36     480
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     140     516 
   Size:        140     376
 
BbvCI CC'TCA_GC

Cuts at:      0     317     516 
   Size:        317     199
 
BglII A'GATC_T

Cuts at:      0     186     516 
   Size:        186     330
 
Bme1580I G_kGCm'C

Cuts at:      0      36     516 
   Size:         36     480
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79      83     196     516 
   Size:         79       4     113     320

  Fragments arranged by size: 

                 320     113      79       4
 
Bpu10I CC'TnA_GC

Cuts at:      0     317     516 
   Size:        317     199
 
BsaHI Gr'CG_yC

Cuts at:      0      76     516 
   Size:         76     440
 
BsaWI w'CCGG_w

Cuts at:      0      24     516 
   Size:         24     492
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     194     224     516 
   Size:        194      30     292

  Fragments arranged by size: 

                 292     194      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     408     516 
   Size:        408     108
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      26     516 
   Size:         26     490
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     408     516 
   Size:         36     372     108

  Fragments arranged by size: 

                 372     108      36
 
BspEI T'CCGG_A

Cuts at:      0      24     516 
   Size:         24     492
 
BspHI T'CATG_A

Cuts at:      0     375     516 
   Size:        375     141
 
BstYI r'GATC_y

Cuts at:      0     186     516 
   Size:        186     330
 
BtsI GCAGTG_nn'

Cuts at:      0     149     516 
   Size:        149     367
 
DraI TTT'AAA

Cuts at:      0     276     516 
   Size:        276     240
 
EaeI y'GGCC_r

Cuts at:      0      43     516 
   Size:         43     473
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     431     516 
   Size:        418      13      85

  Fragments arranged by size: 

                 418      85      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79      83     196     516 
   Size:         79       4     113     320

  Fragments arranged by size: 

                 320     113      79       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32      33     516 
   Size:         32       1     483

  Fragments arranged by size: 

                 483      32       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     110     184     516 
   Size:        110      74     332

  Fragments arranged by size: 

                 332     110      74
 
HincII GTy'rAC

Cuts at:      0     491     516 
   Size:        491      25
 
HpaI GTT'AAC

Cuts at:      0     491     516 
   Size:        491      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     422     516 
   Size:        275     147      94

  Fragments arranged by size: 

                 275     147      94
 
MslI CAynn'nnrTG

Cuts at:      0     332     516 
   Size:        332     184
 
PasI CC'CwG_GG

Cuts at:      0      10     516 
   Size:         10     506
 
PfoI T'CCnGG_A

Cuts at:      0     212     516 
   Size:        212     304
 
PspOMI G'GGCC_C

Cuts at:      0      32     516 
   Size:         32     484
 
PstI C_TGCA'G

Cuts at:      0     194     419     516 
   Size:        194     225      97

  Fragments arranged by size: 

                 225     194      97
 
SfcI C'TryA_G

Cuts at:      0     128     190     415     516 
   Size:        128      62     225     101

  Fragments arranged by size: 

                 225     128     101      62
 

 Enzymes that do cut:

     AlfI     ApaI     ApoI     AseI    BanII     BbsI    BbvCI    BglII
 Bme1580I     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BstYI     BtsI     DraI     EaeI     EarI
  Eco57MI EcoO109I     FalI   HincII     HpaI     MmeI     MslI     PasI
     PfoI   PspOMI     PstI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI