Restriction map for
pF1KB6836
(Linear) (Six-base) MAPSORT of: KIBB6836.seq Check: 3916 from: 1 to: 525
KIBB6836.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 278 525
Size: 220 58 247
Fragments arranged by size:
247 220 58
AleI CACnn'nnGTG
Cuts at: 0 34 525
Size: 34 491
BanII G_rGCy'C
Cuts at: 0 7 525
Size: 7 518
BmgBI CAC'GTC
Cuts at: 0 36 525
Size: 36 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 409 525
Size: 328 81 116
Fragments arranged by size:
328 116 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 525
Size: 499 26
BsaHI Gr'CG_yC
Cuts at: 0 284 525
Size: 284 241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 525
Size: 184 341
BseYI C'CCAG_C
Cuts at: 0 462 478 483 525
Size: 462 16 5 42
Fragments arranged by size:
462 42 16 5
BsiEI CG_ry'CG
Cuts at: 0 117 225 525
Size: 117 108 300
Fragments arranged by size:
300 117 108
BsiHKAI G_wGCw'C
Cuts at: 0 7 525
Size: 7 518
BsmBI CGTCTCn'nnnn_
Cuts at: 0 199 525
Size: 199 326
Bsp1286I G_dGCh'C
Cuts at: 0 7 525
Size: 7 518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 525
Size: 80 445
BstBI TT'CG_AA
Cuts at: 0 500 525
Size: 500 25
BstEII G'GTnAC_C
Cuts at: 0 436 525
Size: 436 89
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 306 525
Size: 36 270 219
Fragments arranged by size:
270 219 36
BstYI r'GATC_y
Cuts at: 0 336 525
Size: 336 189
BtgI C'CryG_G
Cuts at: 0 299 525
Size: 299 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 134 525
Size: 134 391
EaeI y'GGCC_r
Cuts at: 0 164 222 525
Size: 164 58 303
Fragments arranged by size:
303 164 58
EagI C'GGCC_G
Cuts at: 0 222 525
Size: 222 303
EarI CTCTTCn'nnn_
Cuts at: 0 238 502 525
Size: 238 264 23
Fragments arranged by size:
264 238 23
EcoICRI GAG'CTC
Cuts at: 0 5 525
Size: 5 520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 278 328 409 525
Size: 220 58 50 81 116
Fragments arranged by size:
220 116 81 58 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 525
Size: 406 119
EcoO109I rG'GnC_Cy
Cuts at: 0 327 525
Size: 327 198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 525
Size: 436 89
HincII GTy'rAC
Cuts at: 0 51 525
Size: 51 474
HindIII A'AGCT_T
Cuts at: 0 418 525
Size: 418 107
MslI CAynn'nnrTG
Cuts at: 0 34 377 525
Size: 34 343 148
Fragments arranged by size:
343 148 34
MspA1I CmG'CkG
Cuts at: 0 256 525
Size: 256 269
NcoI C'CATG_G
Cuts at: 0 299 525
Size: 299 226
NotI GC'GGCC_GC
Cuts at: 0 222 525
Size: 222 303
PasI CC'CwG_GG
Cuts at: 0 348 525
Size: 348 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 525
Size: 462 63
PfoI T'CCnGG_A
Cuts at: 0 236 386 525
Size: 236 150 139
Fragments arranged by size:
236 150 139
PstI C_TGCA'G
Cuts at: 0 77 525
Size: 77 448
PvuII CAG'CTG
Cuts at: 0 256 525
Size: 256 269
SacI G_AGCT'C
Cuts at: 0 7 525
Size: 7 518
SbfI CC_TGCA'GG
Cuts at: 0 77 525
Size: 77 448
SfcI C'TryA_G
Cuts at: 0 73 525
Size: 73 452
SmlI C'TyrA_G
Cuts at: 0 514 525
Size: 514 11
StuI AGG'CCT
Cuts at: 0 79 525
Size: 79 446
StyI C'CwwG_G
Cuts at: 0 299 525
Size: 299 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 454 525
Size: 424 30 71
Fragments arranged by size:
424 71 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 525
Size: 463 62
Enzymes that do cut:
AcuI AleI BanII BmgBI BpmI BpuEI BsaHI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BstBI BstEII
BstXI BstYI BtgI BtgZI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HincII HindIII MslI MspA1I
NcoI NotI PasI PflMI PfoI PstI PvuII SacI
SbfI SfcI SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI