(Linear) (Six-base) MAPSORT of: KIBB6832.seq  Check: 3802  from: 1  to: 567



KIBB6832.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     567 
   Size:        295     272
 
ApoI r'AATT_y

Cuts at:      0     240     567 
   Size:        240     327
 
BanII G_rGCy'C

Cuts at:      0      18     567 
   Size:         18     549
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     121     567 
   Size:        121     446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     567 
   Size:        521      46
 
BsaWI w'CCGG_w

Cuts at:      0     290     567 
   Size:        290     277
 
BseYI C'CCAG_C

Cuts at:      0      85     567 
   Size:         85     482
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     567 
   Size:        338     229
 
BsiHKAI G_wGCw'C

Cuts at:      0      18     567 
   Size:         18     549
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      46     567 
   Size:         46     521
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     567 
   Size:         18     549
 
BspHI T'CATG_A

Cuts at:      0      74     342     567 
   Size:         74     268     225

  Fragments arranged by size: 

                 268     225      74
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     310     567 
   Size:        310     257
 
BstEII G'GTnAC_C

Cuts at:      0      96     567 
   Size:         96     471
 
EcoICRI GAG'CTC

Cuts at:      0      16     567 
   Size:         16     551
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     567 
   Size:        295     272
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297     322     469     567 
   Size:        297      25     147      98

  Fragments arranged by size: 

                 297     147      98      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37     567 
   Size:          5      32     530

  Fragments arranged by size: 

                 530      32       5
 
MfeI C'AATT_G

Cuts at:      0     500     567 
   Size:        500      67
 
NdeI CA'TA_TG

Cuts at:      0     198     567 
   Size:        198     369
 
PpuMI rG'GwC_Cy

Cuts at:      0     322     567 
   Size:        322     245
 
SacI G_AGCT'C

Cuts at:      0      18     567 
   Size:         18     549
 
SfcI C'TryA_G

Cuts at:      0      65     567 
   Size:         65     502
 
SmlI C'TyrA_G

Cuts at:      0     536     567 
   Size:        536      31
 
StuI AGG'CCT

Cuts at:      0     110     567 
   Size:        110     457
 
TatI w'GTAC_w

Cuts at:      0     129     567 
   Size:        129     438
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      15     567 
   Size:         15     552
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BmrI    BpuEI    BsaWI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI   BstEII  EcoICRI  Eco57MI
 EcoO109I    Hin4I     MfeI     NdeI    PpuMI     SacI     SfcI     SmlI
     StuI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI