(Linear) (Six-base) MAPSORT of: KIBB6806.seq  Check: 4414  from: 1  to: 552



KIBB6806.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     514     552 
   Size:        514      38
 
ApaI G_GGCC'C

Cuts at:      0     177     552 
   Size:        177     375
 
BamHI G'GATC_C

Cuts at:      0      97     552 
   Size:         97     455
 
BanI G'GyrC_C

Cuts at:      0      16     552 
   Size:         16     536
 
BanII G_rGCy'C

Cuts at:      0     177     552 
   Size:        177     375
 
BbeI G_GCGC'C

Cuts at:      0      20     552 
   Size:         20     532
 
BbvCI CC'TCA_GC

Cuts at:      0     112     552 
   Size:        112     440
 
BglI GCCn_nnn'nGGC

Cuts at:      0      62     552 
   Size:         62     490
 
BlpI GC'TnA_GC

Cuts at:      0     157     552 
   Size:        157     395
 
Bme1580I G_kGCm'C

Cuts at:      0     177     552 
   Size:        177     375
 
Bpu10I CC'TnA_GC

Cuts at:      0     112     552 
   Size:        112     440
 
BsaHI Gr'CG_yC

Cuts at:      0      17     552 
   Size:         17     535
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     552 
   Size:        412     140
 
BseYI C'CCAG_C

Cuts at:      0      10      82     552 
   Size:         10      72     470

  Fragments arranged by size: 

                 470      72      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     552 
   Size:         58     494
 
BsiEI CG_ry'CG

Cuts at:      0      62      66     154     552 
   Size:         62       4      88     398

  Fragments arranged by size: 

                 398      88      62       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     177     552 
   Size:        177     375
 
BsrFI r'CCGG_y

Cuts at:      0      61      65     149     552 
   Size:         61       4      84     403

  Fragments arranged by size: 

                 403      84      61       4
 
BssHII G'CGCG_C

Cuts at:      0      30     552 
   Size:         30     522
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     537     552 
   Size:        537      15
 
BstYI r'GATC_y

Cuts at:      0      97     552 
   Size:         97     455
 
EaeI y'GGCC_r

Cuts at:      0      59      63     552 
   Size:         59       4     489

  Fragments arranged by size: 

                 489      59       4
 
EagI C'GGCC_G

Cuts at:      0      59      63     552 
   Size:         59       4     489

  Fragments arranged by size: 

                 489      59       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86     552 
   Size:         86     466
 
FseI GG_CCGG'CC

Cuts at:      0      65     552 
   Size:         65     487
 
HaeII r_GCGC'y

Cuts at:      0      20     552 
   Size:         20     532
 
KasI G'GCGC_C

Cuts at:      0      16     552 
   Size:         16     536
 
MslI CAynn'nnrTG

Cuts at:      0     368     535     552 
   Size:        368     167      17

  Fragments arranged by size: 

                 368     167      17
 
MspA1I CmG'CkG

Cuts at:      0      10     104     552 
   Size:         10      94     448

  Fragments arranged by size: 

                 448      94      10
 
NaeI GCC'GGC

Cuts at:      0      63     552 
   Size:         63     489
 
NarI GG'CG_CC

Cuts at:      0      17     552 
   Size:         17     535
 
NdeI CA'TA_TG

Cuts at:      0     423     552 
   Size:        423     129
 
NgoMIV G'CCGG_C

Cuts at:      0      61     552 
   Size:         61     491
 
NspI r_CATG'y

Cuts at:      0     317     411     552 
   Size:        317      94     141

  Fragments arranged by size: 

                 317     141      94
 
PpuMI rG'GwC_Cy

Cuts at:      0      86     552 
   Size:         86     466
 
PsiI TTA'TAA

Cuts at:      0     214     552 
   Size:        214     338
 
PspOMI G'GGCC_C

Cuts at:      0     173     552 
   Size:        173     379
 
SanDI GG'GwC_CC

Cuts at:      0      86     552 
   Size:         86     466
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      62     552 
   Size:         62     490
 
SfoI GGC'GCC

Cuts at:      0      18     552 
   Size:         18     534
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      70     102     552 
   Size:         70      32     450

  Fragments arranged by size: 

                 450      70      32
 
XbaI T'CTAG_A

Cuts at:      0     291     552 
   Size:        291     261
 

 Enzymes that do cut:

    AlwNI     ApaI    BamHI     BanI    BanII     BbeI    BbvCI     BglI
     BlpI Bme1580I   Bpu10I    BsaHI    BseRI    BseYI     BsgI    BsiEI
 Bsp1286I    BsrFI   BssHII    BstXI    BstYI     EaeI     EagI EcoO109I
     FseI    HaeII     KasI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NspI    PpuMI     PsiI   PspOMI    SanDI     SfiI     SfoI
     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     NcoI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI