(Linear) (Six-base) MAPSORT of: KIBB6792.seq  Check: 9440  from: 1  to: 465



KIBB6792.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     465 
   Size:        395      70
 
AlwNI CAG_nnn'CTG

Cuts at:      0     201     465 
   Size:        201     264
 
ApaLI G'TGCA_C

Cuts at:      0     363     465 
   Size:        363     102
 
AvaI C'yCGr_G

Cuts at:      0     348     409     465 
   Size:        348      61      56

  Fragments arranged by size: 

                 348      61      56
 
BanI G'GyrC_C

Cuts at:      0     271     369     465 
   Size:        271      98      96

  Fragments arranged by size: 

                 271      98      96
 
BanII G_rGCy'C

Cuts at:      0     263     320     465 
   Size:        263      57     145

  Fragments arranged by size: 

                 263     145      57
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322     356     465 
   Size:        322      34     109

  Fragments arranged by size: 

                 322     109      34
 
Bme1580I G_kGCm'C

Cuts at:      0     367     465 
   Size:        367      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     309     465 
   Size:        104     205     156

  Fragments arranged by size: 

                 205     156     104
 
BseYI C'CCAG_C

Cuts at:      0     401     465 
   Size:        401      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     367     465 
   Size:        263     104      98

  Fragments arranged by size: 

                 263     104      98
 
BsmI GAATG_Cn'

Cuts at:      0     432     465 
   Size:        432      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     263     320     367     465 
   Size:        263      57      47      98

  Fragments arranged by size: 

                 263      98      57      47
 
BsrFI r'CCGG_y

Cuts at:      0     302     465 
   Size:        302     163
 
BtsI GCAGTG_nn'

Cuts at:      0     463     465 
   Size:        463       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     465 
   Size:        113     352
 
EcoICRI GAG'CTC

Cuts at:      0     261     465 
   Size:        261     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     309     395     465 
   Size:        104     205      86      70

  Fragments arranged by size: 

                 205     104      86      70
 
HaeII r_GCGC'y

Cuts at:      0      19     465 
   Size:         19     446
 
NspI r_CATG'y

Cuts at:      0      97     465 
   Size:         97     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     143     465 
   Size:        143     322
 
SacI G_AGCT'C

Cuts at:      0     263     465 
   Size:        263     202
 
SmaI CCC'GGG

Cuts at:      0     350     465 
   Size:        350     115
 
SphI G_CATG'C

Cuts at:      0      97     465 
   Size:         97     368
 
StyI C'CwwG_G

Cuts at:      0     275     465 
   Size:        275     190
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     415     465 
   Size:        166     249      50

  Fragments arranged by size: 

                 249     166      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     390     422     465 
   Size:        390      32      43

  Fragments arranged by size: 

                 390      43      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      79     144     465 
   Size:         79      65     321

  Fragments arranged by size: 

                 321      79      65
 
XmaI C'CCGG_G

Cuts at:      0     348     465 
   Size:        348     117
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     AvaI     BanI    BanII     BcgI Bme1580I
     BpmI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrFI     BtsI     EarI
  EcoICRI  Eco57MI    HaeII     NspI    PflMI     SacI     SmaI     SphI
     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI